BBa_K1059027 1 BBa_K1059027 GFP-LVA under RNA guardian controld by exonuclease. 2013-09-16T11:00:00Z 2015-05-08T01:08:57Z The GFP-LVA is from K145015. This coding cosists of a RBS ,an examing protein GFP-LVA,a start codon and several stop codons in different reading frames. It can be used in the 3'-UTR of a mRNA, preventing the mRNA degradation mediated by exonuclease. The extra RBS can attract ribosome to bind on it,which can prevent the mRNA from being degraded by exonuclease . The start codon makes binding more efficient and the stop codon just prevents the DNA???s translation. The circuit is on the backbone of pSB1AK3.We can test the efficiency of the BBa_K1059004 through this circuit. false false _1367_ 0 15632 9 It's complicated false The "extra RBS " and the "real RBS " must be enough interval, preventing the ribosome interfe with each other.And we can test the efficiency of the BBa_K1059004 through this circuit. false Qiu Wang annotation2352872 1 K 1059004 range2352872 1 790 878 annotation2352857 1 B0015 range2352857 1 885 1013 annotation2352856 1 GFP-LVA range2352856 1 23 781 annotation2352855 1 B0035 range2352855 1 3 22 BBa_K1059027_sequence 1 atattaaagaggagaataaataatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagcgcttcgcacctaggatctatgctagcattaaagaggagaagaaagaatgtaataggtgagtagccgtacgataccagcgattatgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z