BBa_K1059066 1 BBa_K1059066 GFP-LVA under RNA guardian control 2013-09-16T11:00:00Z 2015-05-08T01:08:57Z The GFP-LVA sequence is from part 145280, but we change the RBS. And we add K1509004 between B0015 and GFP-LVA, add k1509001 before GFP-LVA. This device consists of a GFP with LVA tag under the control of B0035. At the 3???-UTR and 5???-UTR of the part , we add part K1059004 and K1509001 to stabilize its mRNA to improve the expression of the gene. The end of the gene is terminator B0015. false false _1367_ 0 15654 9 It's complicated true false Yu Wang annotation2352903 1 K1059004 range2352903 1 833 919 annotation2352904 1 B0015 range2352904 1 928 1056 annotation2352898 1 J23101 range2352898 1 1 35 annotation2352902 1 stop codon range2352902 1 816 821 annotation2352899 1 B0035 range2352899 1 46 65 annotation2352900 1 GFP-LVA range2352900 1 66 821 annotation2352901 1 start codon range2352901 1 66 68 BBa_K1059066_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagatattaaagaggagaataaataatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagcgcttcgcacctaggatctatgctagcattaaagaggagaagaaagaatgtaataggtgagtagccgtacgataccagcgattatgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z