BBa_K1059099 1 BBa_K1059099 GFP-LVA under RNA guardian control 2013-09-16T11:00:00Z 2015-05-08T01:08:57Z The GFP-LVA sequence is from part 145280, but we change the RBS. And we add K1509004 between B0015 and GFP-LVA, add k1509001 before GFP-LVA. This device consists of a GFP with LVA tag under the control of B0035. At the 3???-UTR and 5???-UTR of the part , we add part K1059004 and K1509001 to stabilize its mRNA to improve the expression of the gene. The end of the gene is terminator B0015. false false _1367_ 0 15654 9 It's complicated false false Yu Wang annotation2352953 1 stop codon range2352953 1 876 881 annotation2352951 1 GFP-LVA range2352951 1 126 881 annotation2352952 1 K1059004 range2352952 1 885 979 annotation2352950 1 start codon range2352950 1 106 108 annotation2352947 1 J23101 range2352947 1 1 35 annotation2352954 1 B0015 range2352954 1 988 1116 annotation2352949 1 B0035 range2352949 1 106 125 annotation2352948 1 K1059003 range2352948 1 1 95 BBa_K1059099_sequence 1 tttacagctagctcagtcctaggtattatgctagcattaaagaggagaagaaagaatgtaataggtgagtagccgtacgataccagcgattatgctactagagatattaaagaggagaataaataatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagcgcttcgcacctaggatctatgctagcattaaagaggagaagaaagaatgtaataggtgagtagccgtacgataccagcgattatgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z