LOCUS BBa_K1060003 3255 bp DNA linear 16-Dec-2024 DEFINITION Methyl Salicylate Production ACCESSION BBa_K1060003 VERSION BBa_K1060003.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 CDS 82..1155 /label=BMST1 misc_feature 82..84 /label=start codon misc_feature 1153..1155 /label=stop codon CDS 82..1155 /label=BBa_J45004 stem_loop 1175..1218 /label=stem_loop misc_feature 1164..1243 /label=BBa_B0010 terminator 1164..1243 /label=BBa_B0010 stem_loop 1259..1278 /label=T7 TE misc_feature 1285..1285 /label=stop polyA_site 1279..1292 /label=polya misc_feature 1252..1292 /label=BBa_B0012 terminator 1252..1292 /label=BBa_B0012 misc_feature 1303..1319 /label=lac O1 promoter 1320..1325 /label=-35 misc_feature 1326..1342 /label=lac O1 promoter 1343..1348 /label=-10 misc_feature 1301..1354 /label=BBa_R0011 promoter 1301..1354 /label=BBa_R0011 RBS 1364..1376 /label=RBS-3\Weak RBS 1370..1373 /label=RBS misc_feature 1364..1376 /label=BBa_B0032 RBS 1364..1376 /label=BBa_B0032 misc_feature 1822..1822 /label=g -> a to eliminate PstI site misc_feature 1685..1687 /label=pchA start codon misc_feature 1383..1385 /label=pchB start codon misc_feature 1686..1688 /label=pchB stop codon misc_feature 3113..3118 /label=pchA double TAA stop codon CDS 1383..1688 /label=PchB CDS 1685..3118 /label=PchA CDS 1383..3118 /label=BBa_J45017 stem_loop 3138..3181 /label=stem_loop misc_feature 3127..3206 /label=BBa_B0010 terminator 3127..3206 /label=BBa_B0010 stem_loop 3222..3241 /label=T7 TE misc_feature 3248..3248 /label=stop polyA_site 3242..3255 /label=polya misc_feature 3215..3255 /label=BBa_B0012 terminator 3215..3255 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaagtacta gatggaagtt gttgaagttc ttcacatgaa tggaggaaat 121 ggagacagta gctatgcaaa caattctttg gttcagcaaa aggtgattct catgacaaag 181 ccaataactg agcaagccat gattgatctc tacagcagcc tctttccaga aaccttatgc 241 attgcagatt tgggttgttc tttgggagct aacactttct tggtggtctc acagcttgtt 301 aaaatagtag aaaaagaacg aaaaaagcat ggttttaagt ctccagagtt ttattttcac 361 ttcaatgatc ttcctggcaa tgattttaat acactttttc agtcactggg ggcatttcaa 421 gaagatttga gaaagcatat aggggaaagc tttggtccat gttttttcag tggagtgcct 481 ggttcatttt atactagact tttcccttcc aaaagtttac attttgttta ctcctcctac 541 agtctcatgt ggctatctca ggtgcctaat gggattgaaa ataacaaggg aaacatttac 601 atggcaagaa caagccctct aagtgttatt aaagcatact acaagcaata tgaaatagat 661 ttttcaaatt ttctcaagta ccgttcagag gaattgatga aaggtggaaa gatggtgtta 721 acactcctag gtagagaaag tgaggatcct actagcaaag aatgctgtta catttgggag 781 cttctagcca tggccctcaa taagttggtt gaagagggat tgataaaaga agagaaagta 841 gatgcattca atattcctca atacacacca tcaccagcag aagtaaagta catagttgag 901 aaggaaggat cattcaccat taatcgcttg gaaacatcaa gagttcattg gaatgcttct 961 aataatgaga agaatggtgg ttacaatgtg tcaaggtgca tgagagctgt ggctgagcct 1021 ttgcttgtca gccactttga caaggaattg atggatttag tgttccacaa gtacgaagag 1081 attgtttctg attgcatgtc caaagagaat actgagttta taaatgtcat catctccttg 1141 accaaaataa attaatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 1201 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 1261 ctcaccttcg ggtgggcctt tctgcgttta tatactagag aattgtgagc ggataacaat 1321 tgacattgtg agcggataac aagatactga gcacatacta gagtcacaca ggaaagtact 1381 agatgaaaac tcccgaagac tgcaccggcc tggcggacat ccgcgaggcc atcgaccgga 1441 tcgacctgga tatcgtccag gccctcggcc gccgcatgga ctacgtcaag gcggcgtcgc 1501 gcttcaaggc cagcgaggcg gcgattccgg cgcccgagcg ggtcgccgcg atgctccccg 1561 agcgcgcccg ctgggccgag gaaaacggac tcgacgcgcc cttcgtcgag ggactgttcg 1621 cgcagatcat ccactggtac atcgccgagc agatcaagta ctggcgccag acacggggtg 1681 ccgcatgagc cggctggcgc ccctgagcca gtgcctgcac gccttgcgcg gcaccttcga 1741 gcgcgccatc ggccaggcgc aggcgctcga tcgtccggtg ctggtggcgg catcgttcga 1801 gatcgaccca ttggacccgc tacaggtatt cggtgcctgg gacgaccggc aaacgccctg 1861 cctgtactgg gaacagcccg agctggcgtt cttcgcctgg ggctgcgccc tggagctgca 1921 aggccacggc gaacagcgct tcgcccggat cgaggaaaac tggcaattgc tctgcgccga 1981 cgccgtggtc gagggcccgc tggcgccgcg cctgtgcggc ggattccgct tcgatccgcg 2041 cggcccgcgc gaggaacact ggcaagcctt cgccgatgcc agcctgatgc tcgccggcat 2101 caccgtgctg cgcgagggcg aacgctaccg ggtactctgc caacacctgg ccaagcccgg 2161 cgaagatgcc ctggccctgg ccgcctacca ctgctcggcg ctactgcgcc tgaggcagcc 2221 ggccagacgc cggccctcgg ggccgaccgc tggcgcgcag ggcgacgctt cggcgcagga 2281 gcgcaggcaa tgggaagcca aggtgagcga cgcggtaagc agtgtccgcc agggacgctt 2341 cggcaaggtc gtgctggccc gcacccaggc ccggcctctc ggcgacatcg agccgtggca 2401 ggtcatcgaa cacctgcgtc tgcaacatgc cgacgcccag ctgttcgcct gtcgccgcgg 2461 caacgcctgc ttcctcggcg cctccccgga acgcctggtc cgcattcgcg ccggcgaggc 2521 actcacccat gccctggccg ggaccatcgc ccgcggcggc gatgcccagg aagatgcgcg 2581 gctcggacag gccctgctgg acagcgccaa ggacaggcac gaacaccagt tggtggtgga 2641 ggcgatccgt acggccctgg aacccttcag cgaggtgctg gaaatccccg atgcgcccgg 2701 cctgaaacga ctggcgcgag tccagcacct gaacacgccg atccgcgccc gcctcgctga 2761 cgcaggcggc atcctgcggc tgctacaagc gctgcatccg acccccgcgg tgggcggcta 2821 cccacgcagc gcggcgctgg actacatccg ccagcacgaa gggatggacc gcggctggta 2881 cgccgcgccg ctgggctggc tcgacggcga aggcaacggc gatttcctgg tggcgctgcg 2941 ctcggccctg ctcacgccgg gccggggcta cctgttcgcc ggctgcggtc tggtaggcga 3001 ttcggaaccg gcccacgagt atcgcgaaac ctgccttaag ctcagtgcca tgcgggaagc 3061 tctatccgcc ataggcggcc tggacgaagt gcccttgcag cgcggcgtcg cctaataata 3121 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 3181 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 3241 ctttctgcgt ttata //