BBa_K1060003
type: ComponentDefinition
persistent identity: BBa K1060003
display id: BBa_K1060003
version: 1
was generated by: iGEM to SBOL conversion
description: Methyl Salicylate Production
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:57Z
mutable provenance: BBa_J45700 (MIT 2006)
owned by: james
owned by: myers
mutable description: KU_Leuven 2013 requested the BBa_J45700 brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone. For the assembly of this part we refer to the original MIT brick BBa_J45700 => "We assembled a complete wintergreen odor biosynthetic system (BBa_J45700) by combining the salicylic acid (BBa_J45320) and wintergreen odor generators (BBa_J45120). BBa_J45700 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor."
discontinued: false
dominant: false
experience: experience/None
group u list: _1368_
m user id: 0
owner id: 17634
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no EcoRI, XbaI, NotI, SpeI or PstI sites present
star: false
creator: Ingmar Claes, Robbert Boudewijns, Laurens De Backer
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_J45004
component: BBa_B0032
component: BBa_J45017
component: BBa_R0011
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_J45004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_J45017
sequence: https://synbiohub.org/public/igem/BBa_K1060003_sequence/1

BBa_B0012
type: Component
persistent identity: component2349871
display id: component2349871
version: 1
top level: BBa_K1060003
definition: BBa_B0012
access: public

BBa_B0012
type: Component
persistent identity: component2349849
display id: component2349849
version: 1
top level: BBa_K1060003
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2349847
display id: component2349847
version: 1
top level: BBa_K1060003
definition: BBa_B0010
access: public

BBa_J45004
type: Component
persistent identity: component2349846
display id: component2349846
version: 1
top level: BBa_K1060003
definition: BSMT1
access: public

BBa_B0032
type: Component
persistent identity: component2349841
display id: component2349841
version: 1
top level: BBa_K1060003
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component2349869
display id: component2349869
version: 1
top level: BBa_K1060003
definition: BBa_B0010
access: public

BBa_J45017
type: Component
persistent identity: component2349868
display id: component2349868
version: 1
top level: BBa_K1060003
definition: pchBA
access: public

BBa_B0032
type: Component
persistent identity: component2349859
display id: component2349859
version: 1
top level: BBa_K1060003
definition: BBa_B0032
access: public

BBa_R0011
type: Component
persistent identity: component2349853
display id: component2349853
version: 1
top level: BBa_K1060003
definition: lacI+pL
access: public

BBa_R0040
type: Component
persistent identity: component2349835
display id: component2349835
version: 1
top level: BBa_K1060003
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2349835
display id: annotation2349835
version: 1
top level: BBa_K1060003
location: https://synbiohub.org/public/igem/BBa_K1060003/annotation2349835/range2349835/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1060003/annotation2349835/range2349835/1
type: Range
persistent identity: range2349835
display id: range2349835
version: 1
top level: BBa_K1060003
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2349841
display id: annotation2349841
version: 1
top level: BBa_K1060003
location: https://synbiohub.org/public/igem/BBa_K1060003/annotation2349841/range2349841/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1060003/annotation2349841/range2349841/1
type: Range
persistent identity: range2349841
display id: range2349841
version: 1
top level: BBa_K1060003
direction: direction/forward
start: 63
end: 75
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation2349846
display id: annotation2349846
version: 1
top level: BBa_K1060003
location: https://synbiohub.org/public/igem/BBa_K1060003/annotation2349846/range2349846/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_K1060003/annotation2349846/range2349846/1
type: Range
persistent identity: range2349846
display id: range2349846
version: 1
top level: BBa_K1060003
direction: direction/reverse
start: 82
end: 1155
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2349847
display id: annotation2349847
version: 1
top level: BBa_K1060003
location: https://synbiohub.org/public/igem/BBa_K1060003/annotation2349847/range2349847/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1060003/annotation2349847/range2349847/1
type: Range
persistent identity: range2349847
display id: range2349847
version: 1
top level: BBa_K1060003
direction: direction/forward
start: 1164
end: 1243
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2349849
display id: annotation2349849
version: 1
top level: BBa_K1060003
location: https://synbiohub.org/public/igem/BBa_K1060003/annotation2349849/range2349849/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1060003/annotation2349849/range2349849/1
type: Range
persistent identity: range2349849
display id: range2349849
version: 1
top level: BBa_K1060003
direction: direction/forward
start: 1252
end: 1292
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2349853
display id: annotation2349853
version: 1
top level: BBa_K1060003
location: https://synbiohub.org/public/igem/BBa_K1060003/annotation2349853/range2349853/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1060003/annotation2349853/range2349853/1
type: Range
persistent identity: range2349853
display id: range2349853
version: 1
top level: BBa_K1060003
direction: direction/forward
start: 1301
end: 1354
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2349859
display id: annotation2349859
version: 1
top level: BBa_K1060003
location: https://synbiohub.org/public/igem/BBa_K1060003/annotation2349859/range2349859/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1060003/annotation2349859/range2349859/1
type: Range
persistent identity: range2349859
display id: range2349859
version: 1
top level: BBa_K1060003
direction: direction/forward
start: 1364
end: 1376
orientation: inline

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation2349868
display id: annotation2349868
version: 1
top level: BBa_K1060003
location: https://synbiohub.org/public/igem/BBa_K1060003/annotation2349868/range2349868/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_K1060003/annotation2349868/range2349868/1
type: Range
persistent identity: range2349868
display id: range2349868
version: 1
top level: BBa_K1060003
direction: direction/reverse
start: 1383
end: 3118
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2349869
display id: annotation2349869
version: 1
top level: BBa_K1060003
location: https://synbiohub.org/public/igem/BBa_K1060003/annotation2349869/range2349869/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1060003/annotation2349869/range2349869/1
type: Range
persistent identity: range2349869
display id: range2349869
version: 1
top level: BBa_K1060003
direction: direction/forward
start: 3127
end: 3206
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2349871
display id: annotation2349871
version: 1
top level: BBa_K1060003
location: https://synbiohub.org/public/igem/BBa_K1060003/annotation2349871/range2349871/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1060003/annotation2349871/range2349871/1
type: Range
persistent identity: range2349871
display id: range2349871
version: 1
top level: BBa_K1060003
direction: direction/forward
start: 3215
end: 3255
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

pchBA
type: ComponentDefinition
persistent identity: BBa J45017
display id: BBa_J45017
version: 1
was generated by: iGEM to SBOL conversion
description: isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate
created: 2006-08-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: pchBA is found in pseudomonas. The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Riemmann from the Universite de Lausanne.
owned by: james
owned by: myers
mutable description: pchBA codes for proteins used to generate salicylic acid. It doesn't make a fusion protein, even though the coding regions overlap; the RBS for pchA is located at the end of the coding region of pchB.
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: n/a
star: false
creator: MIT IGEM 2006
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pchB start codon
sequence annotation: PchB
sequence annotation: pchA start codon
sequence annotation: PchA
sequence annotation: pchB stop codon
sequence annotation: g -> a to eliminate PstI site
sequence annotation: pchA double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45017_sequence/1

pchB start codon
type: SequenceAnnotation
persistent identity: annotation1897455
display id: annotation1897455
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897455/range1897455/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J45017/annotation1897455/range1897455/1
type: Range
persistent identity: range1897455
display id: range1897455
version: 1
top level: pchBA
direction: direction/forward
start: 1
end: 3
orientation: inline

PchB
type: SequenceAnnotation
persistent identity: annotation1961296
display id: annotation1961296
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1961296/range1961296/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J45017/annotation1961296/range1961296/1
type: Range
persistent identity: range1961296
display id: range1961296
version: 1
top level: pchBA
direction: direction/forward
start: 1
end: 306
orientation: inline

pchA start codon
type: SequenceAnnotation
persistent identity: annotation1897454
display id: annotation1897454
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897454/range1897454/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J45017/annotation1897454/range1897454/1
type: Range
persistent identity: range1897454
display id: range1897454
version: 1
top level: pchBA
direction: direction/forward
start: 303
end: 305
orientation: inline

PchA
type: SequenceAnnotation
persistent identity: annotation1961297
display id: annotation1961297
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1961297/range1961297/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J45017/annotation1961297/range1961297/1
type: Range
persistent identity: range1961297
display id: range1961297
version: 1
top level: pchBA
direction: direction/forward
start: 303
end: 1736
orientation: inline

pchB stop codon
type: SequenceAnnotation
persistent identity: annotation1897456
display id: annotation1897456
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897456/range1897456/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J45017/annotation1897456/range1897456/1
type: Range
persistent identity: range1897456
display id: range1897456
version: 1
top level: pchBA
direction: direction/forward
start: 304
end: 306
orientation: inline

g -> a to eliminate PstI site
type: SequenceAnnotation
persistent identity: annotation1897453
display id: annotation1897453
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897453/range1897453/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_J45017/annotation1897453/range1897453/1
type: Range
persistent identity: range1897453
display id: range1897453
version: 1
top level: pchBA
direction: direction/forward
start: 440
end: 440
orientation: inline

pchA double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1897457
display id: annotation1897457
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897457/range1897457/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J45017/annotation1897457/range1897457/1
type: Range
persistent identity: range1897457
display id: range1897457
version: 1
top level: pchBA
direction: direction/forward
start: 1731
end: 1736
orientation: inline

BSMT1
type: ComponentDefinition
persistent identity: BBa J45004
display id: BBa_J45004
version: 1
was generated by: iGEM to SBOL conversion
description: SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: Species: Petunia x hybrida from Genbank; (also available: A. thaliana and N. suaveolens)
owned by: james
owned by: myers
mutable description: Codes for BSMT enzyme to convert Benzoic acid/salicylic acid to methyl benzoate and methyl salicylate. This part is phBSMT1 from Petunia x hybrida.
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Transport issues are being considered.
star: true
creator: Boyuan Zhu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BMST1
sequence annotation: start codon
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45004_sequence/1

BMST1
type: SequenceAnnotation
persistent identity: annotation1880517
display id: annotation1880517
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1
type: Range
persistent identity: range1880517
display id: range1880517
version: 1
top level: BSMT1
direction: direction/forward
start: 1
end: 1074
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1880519
display id: annotation1880519
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1
type: Range
persistent identity: range1880519
display id: range1880519
version: 1
top level: BSMT1
direction: direction/forward
start: 1
end: 3
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1880520
display id: annotation1880520
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1
type: Range
persistent identity: range1880520
display id: range1880520
version: 1
top level: BSMT1
direction: direction/forward
start: 1072
end: 1074
orientation: inline

https://synbiohub.org/public/igem/BBa_K1060003_sequence/1
type: Sequence
persistent identity: BBa K1060003 sequence
display id: BBa_K1060003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaaaataaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45017_sequence/1
type: Sequence
persistent identity: BBa J45017 sequence
display id: BBa_J45017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgccctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgaccgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcatcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctccccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatgcccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggcgatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtccagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccgacccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggtacgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccgggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgccttaagctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45004_sequence/1
type: Sequence
persistent identity: BBa J45004 sequence
display id: BBa_J45004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaaaataaattaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z