##gff-version 3
##sequence-region BBa_K1060005 1 1839
BBa_K1060005	.	terminator	1745	1839	.	+	0	ID=BBa_K823017;Name=BBa_K823017
BBa_K1060005	.	terminator	1745	1839	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1060005	.	terminator	1794	1839	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1060005	.	stem_loop	1806	1828	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1060005	.	engineered_region	1794	1839	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1060005	.	terminator	1745	1785	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1060005	.	engineered_region	1745	1785	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1060005	.	stop_codon	1778	1778	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1060005	.	stem_loop	1752	1771	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1060005	.	polyA_site	1772	1785	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1060005	.	CDS	1	1736	.	+	0	ID=BBa_J45017;Name=BBa_J45017
BBa_K1060005	.	CDS	1	306	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_K1060005	.	start_codon	1	3	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_K1060005	.	point_mutation	440	440	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_K1060005	.	CDS	303	1736	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_K1060005	.	stop_codon	304	306	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_K1060005	.	stop_codon	1731	1736	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_K1060005	.	start_codon	303	305	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
>BBa_K1060005
atgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtccagg
ccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccgagcg
ggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactgttc
gcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggctggc
gcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgtccg
gtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaacgc
cctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcgaaca
gcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgcctg
tgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgctcg
ccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgccct
ggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgaccgct
ggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtccgcc
agggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcatcga
acacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctccccg
gaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatgccc
aggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggcgat
ccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtccag
cacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccgaccc
ccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggtacgc
cgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccgggc
cggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgccttaagc
tcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaataata
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattat
taatccggcttttttattattt