BBa_K1060008 1 BBa_K1060008 K1060002:B0015 2013-09-18T11:00:00Z 2015-05-08T01:08:58Z false false _9_ 0 17634 9 In stock false false Ingmar Claes, Lukas Marcelis, Su Wang component2353972 1 BBa_K1060002 component2353979 1 BBa_B0015 annotation2353972 1 BBa_K1060002 range2353972 1 1 1725 annotation2353979 1 BBa_B0015 range2353979 1 1734 1862 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1060002 1 EbfS Aa EBF synthase from Artemisia annua 2013-09-16T11:00:00Z 2015-05-08T01:08:57Z Artemisia annua genomic DNA (Picaud et al. 2005) This sesquiterpene synthase (GenBank Accession No. AY835398), (E)-b-farnesene synthase, has been isolated from Artemisia annua L (Picaud et al. 2005) false false _1368_ 0 17634 9 In stock true An EcoRI site (513A>C)was removed by site directed mutagenesis false Ingmar Claes, Lukas Marcelis, Su Wang BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1060002_sequence 1 atgtcgactcttcctatttctagtgtttcattctcttcatctacgtcacctttggttgttgacgataaagttagcacgaaaccagatgttatccgccataccatgaatttcaatgctagcatatggggagatcaatttcttacatatgatgagccagaagatttggttatgaagaaacaacttgttgaagagctcaaagaggaagtcaagaaagagctaataacgatcaaaggttcaaatgaaccgatgcagcatgtcaagttgatagaacttattgatgcagtccaacggcttggcatagcctatcattttgaagaggagattgaagaagccttgcaacatatccatgttacatatggcgagcagtgggtcgataaagaaaacctacaaagcatttctctttggttccgactcctacgacaacaaggcttcaacgtctcatcaggagtattcaaggactttatggacgagaagggaaaatttaaggaatccttatgtaatgatgctcagggcattcttgctctgtatgaagcggcatttatgagggtggaagatgaaacaatactagataatgcgctcgagttcactaaagttcaccttgacatcatagccaaggatccttcttgtgactcttccctaagaacccaaatacaccaagcgttaaagcagccacttaggagaaggctggcaaggattgaggcgttgcattacatgcctatctaccaacaagaaacatcccacgatgaggtcttactgaagcttgcaaaattagattttagcgtgcttcagtcgatgcacaaaaaggaacttagccacatttgcaaatggtggaaggatttggacctccaaaataagcttccttatgttcgagacagggtggtggaaggctatttttggatactatccatctattacgagcctcaacatgctcgaacaagaatgttcctaatgaaaacatgcatgtggttagtcgttttagatgatacatttgataattatggtacttatgaagaactcgaaatctttacacaagctgttgaaagatggtcaataagttgcctggatatgcttccagaatacatgaaactaatatatcaagagcttgtgaatcttcacgtggaaatggaggaatcacttgaaaaggagggaaaaacatatcaaattcactatgtcaaggagatggcaaaagagttggttcgcaattacttggtagaagccagatggctaaaagaggggtacatgccaactcttgaggagtacatgtctgtgtcaatggtgactggtacctatggcttgatgatagcgagatcttatgtcggcaggggtgatatcgtcaccgaggatacctttaaatgggtgtcctcgtatcctcctattataaaagcttcatgtgtgattgtaagacttatggatgatattgtcagccacaaggaggaacaagagagaggccatgttgcttcaagcatcgaatgctattctaaggaaagtggtgcatcagaggaggaagcgtgtgaatatatctcaagaaaagttgaagatgcatggaaagctataaaccgagagtcgctcaggcctacagctgtcccatttcctctacttatgcctgcaatcaaccttgcacgtatgtgtgaagtcctatatagcgtcaacgatggcttcactcatgctgagggagacatgaaaagttacatgaaatcgttcttcgttcaccctatggttgtctaa BBa_K1060008_sequence 1 atgtcgactcttcctatttctagtgtttcattctcttcatctacgtcacctttggttgttgacgataaagttagcacgaaaccagatgttatccgccataccatgaatttcaatgctagcatatggggagatcaatttcttacatatgatgagccagaagatttggttatgaagaaacaacttgttgaagagctcaaagaggaagtcaagaaagagctaataacgatcaaaggttcaaatgaaccgatgcagcatgtcaagttgatagaacttattgatgcagtccaacggcttggcatagcctatcattttgaagaggagattgaagaagccttgcaacatatccatgttacatatggcgagcagtgggtcgataaagaaaacctacaaagcatttctctttggttccgactcctacgacaacaaggcttcaacgtctcatcaggagtattcaaggactttatggacgagaagggaaaatttaaggaatccttatgtaatgatgctcagggcattcttgctctgtatgaagcggcatttatgagggtggaagatgaaacaatactagataatgcgctcgagttcactaaagttcaccttgacatcatagccaaggatccttcttgtgactcttccctaagaacccaaatacaccaagcgttaaagcagccacttaggagaaggctggcaaggattgaggcgttgcattacatgcctatctaccaacaagaaacatcccacgatgaggtcttactgaagcttgcaaaattagattttagcgtgcttcagtcgatgcacaaaaaggaacttagccacatttgcaaatggtggaaggatttggacctccaaaataagcttccttatgttcgagacagggtggtggaaggctatttttggatactatccatctattacgagcctcaacatgctcgaacaagaatgttcctaatgaaaacatgcatgtggttagtcgttttagatgatacatttgataattatggtacttatgaagaactcgaaatctttacacaagctgttgaaagatggtcaataagttgcctggatatgcttccagaatacatgaaactaatatatcaagagcttgtgaatcttcacgtggaaatggaggaatcacttgaaaaggagggaaaaacatatcaaattcactatgtcaaggagatggcaaaagagttggttcgcaattacttggtagaagccagatggctaaaagaggggtacatgccaactcttgaggagtacatgtctgtgtcaatggtgactggtacctatggcttgatgatagcgagatcttatgtcggcaggggtgatatcgtcaccgaggatacctttaaatgggtgtcctcgtatcctcctattataaaagcttcatgtgtgattgtaagacttatggatgatattgtcagccacaaggaggaacaagagagaggccatgttgcttcaagcatcgaatgctattctaaggaaagtggtgcatcagaggaggaagcgtgtgaatatatctcaagaaaagttgaagatgcatggaaagctataaaccgagagtcgctcaggcctacagctgtcccatttcctctacttatgcctgcaatcaaccttgcacgtatgtgtgaagtcctatatagcgtcaacgatggcttcactcatgctgagggagacatgaaaagttacatgaaatcgttcttcgttcaccctatggttgtctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z