LOCUS       BBa_K1060009            1924 bp    DNA     linear       12-May-2024
DEFINITION  Medium constitutive expression of EBF synthase from Artemisia annua
ACCESSION   BBa_K1060009
VERSION     BBa_K1060009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    1..56
                     /label=BBa_K608006
     CDS             63..1787
                     /label=BBa_K1060002
     stem_loop       1807..1850
                     /label=stem_loop
     misc_feature    1796..1875
                     /label=BBa_B0010
     terminator      1796..1875
                     /label=BBa_B0010
     stem_loop       1891..1910
                     /label=T7 TE
     misc_feature    1917..1917
                     /label=stop
     polyA_site      1911..1924
                     /label=polya
     misc_feature    1884..1924
                     /label=BBa_B0012
     terminator      1884..1924
                     /label=BBa_B0012
     terminator      1796..1924
                     /label=BBa_B0015
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagtcacaca ggaaagtact
       61 agatgtcgac tcttcctatt tctagtgttt cattctcttc atctacgtca cctttggttg
      121 ttgacgataa agttagcacg aaaccagatg ttatccgcca taccatgaat ttcaatgcta
      181 gcatatgggg agatcaattt cttacatatg atgagccaga agatttggtt atgaagaaac
      241 aacttgttga agagctcaaa gaggaagtca agaaagagct aataacgatc aaaggttcaa
      301 atgaaccgat gcagcatgtc aagttgatag aacttattga tgcagtccaa cggcttggca
      361 tagcctatca ttttgaagag gagattgaag aagccttgca acatatccat gttacatatg
      421 gcgagcagtg ggtcgataaa gaaaacctac aaagcatttc tctttggttc cgactcctac
      481 gacaacaagg cttcaacgtc tcatcaggag tattcaagga ctttatggac gagaagggaa
      541 aatttaagga atccttatgt aatgatgctc agggcattct tgctctgtat gaagcggcat
      601 ttatgagggt ggaagatgaa acaatactag ataatgcgct cgagttcact aaagttcacc
      661 ttgacatcat agccaaggat ccttcttgtg actcttccct aagaacccaa atacaccaag
      721 cgttaaagca gccacttagg agaaggctgg caaggattga ggcgttgcat tacatgccta
      781 tctaccaaca agaaacatcc cacgatgagg tcttactgaa gcttgcaaaa ttagatttta
      841 gcgtgcttca gtcgatgcac aaaaaggaac ttagccacat ttgcaaatgg tggaaggatt
      901 tggacctcca aaataagctt ccttatgttc gagacagggt ggtggaaggc tatttttgga
      961 tactatccat ctattacgag cctcaacatg ctcgaacaag aatgttccta atgaaaacat
     1021 gcatgtggtt agtcgtttta gatgatacat ttgataatta tggtacttat gaagaactcg
     1081 aaatctttac acaagctgtt gaaagatggt caataagttg cctggatatg cttccagaat
     1141 acatgaaact aatatatcaa gagcttgtga atcttcacgt ggaaatggag gaatcacttg
     1201 aaaaggaggg aaaaacatat caaattcact atgtcaagga gatggcaaaa gagttggttc
     1261 gcaattactt ggtagaagcc agatggctaa aagaggggta catgccaact cttgaggagt
     1321 acatgtctgt gtcaatggtg actggtacct atggcttgat gatagcgaga tcttatgtcg
     1381 gcaggggtga tatcgtcacc gaggatacct ttaaatgggt gtcctcgtat cctcctatta
     1441 taaaagcttc atgtgtgatt gtaagactta tggatgatat tgtcagccac aaggaggaac
     1501 aagagagagg ccatgttgct tcaagcatcg aatgctattc taaggaaagt ggtgcatcag
     1561 aggaggaagc gtgtgaatat atctcaagaa aagttgaaga tgcatggaaa gctataaacc
     1621 gagagtcgct caggcctaca gctgtcccat ttcctctact tatgcctgca atcaaccttg
     1681 cacgtatgtg tgaagtccta tatagcgtca acgatggctt cactcatgct gagggagaca
     1741 tgaaaagtta catgaaatcg ttcttcgttc accctatggt tgtctaatac tagagccagg
     1801 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1861 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1921 tata
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