BBa_K1060009
1
BBa_K1060009
Medium constitutive expression of EBF synthase from Artemisia annua
2013-09-18T11:00:00Z
2015-05-08T01:08:58Z
Composite part; genomic sequence from EBF synthase from Artemisia annua combined with iGEM bricks. BBa_K608006 + Bba_K1060002 + Bba_B0015
For the production of the alarmhormone,(E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006).
false
false
_1368_
0
17634
9
It's complicated
true
no EcoRI, XbaI, NotI, SpeI or PstI sites present
false
Ingmar Claes, Su Wang, Sylvie Weckx
component2353994
1
BBa_B0015
component2353987
1
BBa_K1060002
component2353986
1
BBa_K608006
annotation2353987
1
BBa_K1060002
range2353987
1
63
1787
annotation2353986
1
BBa_K608006
range2353986
1
1
56
annotation2353994
1
BBa_B0015
range2353994
1
1796
1924
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K608006
1
BBa_K608006
medium Promoter , medium RBS
2011-09-14T11:00:00Z
2015-05-08T01:12:52Z
composite
Released HQ 2013
medium Promotor , medium RBS
false
false
_780_
0
9115
9
In stock
false
composite
false
Julia M??ller
component2128661
1
BBa_B0032
component2128659
1
BBa_J23110
annotation2128659
1
BBa_J23110
range2128659
1
1
35
annotation2128661
1
BBa_B0032
range2128661
1
44
56
BBa_J23110
1
BBa_J23110
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_K1060002
1
EbfS Aa
EBF synthase from Artemisia annua
2013-09-16T11:00:00Z
2015-05-08T01:08:57Z
Artemisia annua genomic DNA (Picaud et al. 2005)
This sesquiterpene synthase (GenBank Accession No. AY835398), (E)-b-farnesene synthase, has been isolated
from Artemisia annua L (Picaud et al. 2005)
false
false
_1368_
0
17634
9
In stock
true
An EcoRI site (513A>C)was removed by site directed mutagenesis
false
Ingmar Claes, Lukas Marcelis, Su Wang
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1060002_sequence
1
atgtcgactcttcctatttctagtgtttcattctcttcatctacgtcacctttggttgttgacgataaagttagcacgaaaccagatgttatccgccataccatgaatttcaatgctagcatatggggagatcaatttcttacatatgatgagccagaagatttggttatgaagaaacaacttgttgaagagctcaaagaggaagtcaagaaagagctaataacgatcaaaggttcaaatgaaccgatgcagcatgtcaagttgatagaacttattgatgcagtccaacggcttggcatagcctatcattttgaagaggagattgaagaagccttgcaacatatccatgttacatatggcgagcagtgggtcgataaagaaaacctacaaagcatttctctttggttccgactcctacgacaacaaggcttcaacgtctcatcaggagtattcaaggactttatggacgagaagggaaaatttaaggaatccttatgtaatgatgctcagggcattcttgctctgtatgaagcggcatttatgagggtggaagatgaaacaatactagataatgcgctcgagttcactaaagttcaccttgacatcatagccaaggatccttcttgtgactcttccctaagaacccaaatacaccaagcgttaaagcagccacttaggagaaggctggcaaggattgaggcgttgcattacatgcctatctaccaacaagaaacatcccacgatgaggtcttactgaagcttgcaaaattagattttagcgtgcttcagtcgatgcacaaaaaggaacttagccacatttgcaaatggtggaaggatttggacctccaaaataagcttccttatgttcgagacagggtggtggaaggctatttttggatactatccatctattacgagcctcaacatgctcgaacaagaatgttcctaatgaaaacatgcatgtggttagtcgttttagatgatacatttgataattatggtacttatgaagaactcgaaatctttacacaagctgttgaaagatggtcaataagttgcctggatatgcttccagaatacatgaaactaatatatcaagagcttgtgaatcttcacgtggaaatggaggaatcacttgaaaaggagggaaaaacatatcaaattcactatgtcaaggagatggcaaaagagttggttcgcaattacttggtagaagccagatggctaaaagaggggtacatgccaactcttgaggagtacatgtctgtgtcaatggtgactggtacctatggcttgatgatagcgagatcttatgtcggcaggggtgatatcgtcaccgaggatacctttaaatgggtgtcctcgtatcctcctattataaaagcttcatgtgtgattgtaagacttatggatgatattgtcagccacaaggaggaacaagagagaggccatgttgcttcaagcatcgaatgctattctaaggaaagtggtgcatcagaggaggaagcgtgtgaatatatctcaagaaaagttgaagatgcatggaaagctataaaccgagagtcgctcaggcctacagctgtcccatttcctctacttatgcctgcaatcaaccttgcacgtatgtgtgaagtcctatatagcgtcaacgatggcttcactcatgctgagggagacatgaaaagttacatgaaatcgttcttcgttcaccctatggttgtctaa
BBa_K1060009_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgtcgactcttcctatttctagtgtttcattctcttcatctacgtcacctttggttgttgacgataaagttagcacgaaaccagatgttatccgccataccatgaatttcaatgctagcatatggggagatcaatttcttacatatgatgagccagaagatttggttatgaagaaacaacttgttgaagagctcaaagaggaagtcaagaaagagctaataacgatcaaaggttcaaatgaaccgatgcagcatgtcaagttgatagaacttattgatgcagtccaacggcttggcatagcctatcattttgaagaggagattgaagaagccttgcaacatatccatgttacatatggcgagcagtgggtcgataaagaaaacctacaaagcatttctctttggttccgactcctacgacaacaaggcttcaacgtctcatcaggagtattcaaggactttatggacgagaagggaaaatttaaggaatccttatgtaatgatgctcagggcattcttgctctgtatgaagcggcatttatgagggtggaagatgaaacaatactagataatgcgctcgagttcactaaagttcaccttgacatcatagccaaggatccttcttgtgactcttccctaagaacccaaatacaccaagcgttaaagcagccacttaggagaaggctggcaaggattgaggcgttgcattacatgcctatctaccaacaagaaacatcccacgatgaggtcttactgaagcttgcaaaattagattttagcgtgcttcagtcgatgcacaaaaaggaacttagccacatttgcaaatggtggaaggatttggacctccaaaataagcttccttatgttcgagacagggtggtggaaggctatttttggatactatccatctattacgagcctcaacatgctcgaacaagaatgttcctaatgaaaacatgcatgtggttagtcgttttagatgatacatttgataattatggtacttatgaagaactcgaaatctttacacaagctgttgaaagatggtcaataagttgcctggatatgcttccagaatacatgaaactaatatatcaagagcttgtgaatcttcacgtggaaatggaggaatcacttgaaaaggagggaaaaacatatcaaattcactatgtcaaggagatggcaaaagagttggttcgcaattacttggtagaagccagatggctaaaagaggggtacatgccaactcttgaggagtacatgtctgtgtcaatggtgactggtacctatggcttgatgatagcgagatcttatgtcggcaggggtgatatcgtcaccgaggatacctttaaatgggtgtcctcgtatcctcctattataaaagcttcatgtgtgattgtaagacttatggatgatattgtcagccacaaggaggaacaagagagaggccatgttgcttcaagcatcgaatgctattctaaggaaagtggtgcatcagaggaggaagcgtgtgaatatatctcaagaaaagttgaagatgcatggaaagctataaaccgagagtcgctcaggcctacagctgtcccatttcctctacttatgcctgcaatcaaccttgcacgtatgtgtgaagtcctatatagcgtcaacgatggcttcactcatgctgagggagacatgaaaagttacatgaaatcgttcttcgttcaccctatggttgtctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K608006_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaag
BBa_B0032_sequence
1
tcacacaggaaag
BBa_J23110_sequence
1
tttacggctagctcagtcctaggtacaatgctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z