LOCUS       BBa_K1060010             866 bp    DNA     linear       25-Nov-2024
DEFINITION  TetR expression under glucose limiting conditions
ACCESSION   BBa_K1060010
VERSION     BBa_K1060010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=Crp-cAMP
     promoter        16..45
                     /label=pCaiF
     promoter        1..51
                     /label=BBa_K398326
     RBS             60..72
                     /label=RBS-3\Weak
     RBS             66..69
                     /label=RBS
     misc_feature    60..72
                     /label=BBa_B0032
     RBS             60..72
                     /label=BBa_B0032
     CDS             82..698
                     /label=tetR
     misc_feature    699..732
                     /label=SsrA
     misc_feature    739..763
                     /label=Help:Barcodes
     CDS             79..763
                     /label=BBa_C0040
     stem_loop       779..798
                     /label=T7 TE
     misc_feature    805..805
                     /label=stop
     polyA_site      799..812
                     /label=polya
     misc_feature    772..812
                     /label=BBa_B0012
     terminator      772..812
                     /label=BBa_B0012
     stem_loop       833..855
                     /label=stem_loop
     misc_feature    821..866
                     /label=BBa_B0011
     terminator      821..866
                     /label=BBa_B0011
     terminator      772..866
                     /label=BBa_B0014
     terminator      772..866
                     /label=BBa_K823017
ORIGIN
        1 aagcaggatt tagctcacac ttatcgacgg tgaagttgca tactatcgat atactagagt
       61 cacacaggaa agtactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
      121 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      181 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      241 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      301 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      361 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      421 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      481 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      541 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      601 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      661 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      721 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gtcacactgg
      781 ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga
      841 ttattaatcc ggctttttta ttattt
//