##gff-version 3
##sequence-region BBa_K1060010 1 866
BBa_K1060010	.	promoter	1	51	.	+	0	ID=BBa_K398326;Name=BBa_K398326
BBa_K1060010	.	promoter	16	45	.	+	0	ID=annotation2090459;Name=pCaiF;Parent=BBa_K398326
BBa_K1060010	.	non_covalent_binding_site	1	21	.	+	0	ID=annotation2090458;Name=Crp-cAMP;Parent=BBa_K398326
BBa_K1060010	.	ribosome_entry_site	60	72	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K1060010	.	ribosome_entry_site	60	72	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1060010	.	engineered_region	60	72	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1060010	.	ribosome_entry_site	66	69	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1060010	.	CDS	79	763	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K1060010	.	engineered_tag	739	763	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1060010	.	CDS	82	698	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1060010	.	tag	699	732	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1060010	.	terminator	772	866	.	+	0	ID=BBa_K823017;Name=BBa_K823017
BBa_K1060010	.	terminator	772	866	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1060010	.	terminator	772	812	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1060010	.	stop_codon	805	805	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1060010	.	polyA_site	799	812	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1060010	.	stem_loop	779	798	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1060010	.	engineered_region	772	812	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1060010	.	terminator	821	866	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1060010	.	engineered_region	821	866	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1060010	.	stem_loop	833	855	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
>BBa_K1060010
aagcaggatttagctcacacttatcgacggtgaagttgcatactatcgatatactagagtcacacaggaaagtactaga
tgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaac
ccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgac
gccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttac
gtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctac
agaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatat
gcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggg
aaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagc
cttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgac
gaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt