LOCUS BBa_K1060012 3222 bp DNA linear 05-May-2025 DEFINITION Methyl Salicylate Production under TetR regulation ACCESSION BBa_K1060012 VERSION BBa_K1060012.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..77 /label=RBS-1\Strong RBS 70..74 /label=RBS misc_feature 63..77 /label=BBa_B0030 RBS 63..77 /label=BBa_B0030 CDS 84..1157 /label=BMST1 misc_feature 84..86 /label=start codon misc_feature 1155..1157 /label=stop codon CDS 84..1157 /label=BBa_J45004 stem_loop 1173..1192 /label=T7 TE misc_feature 1199..1199 /label=stop polyA_site 1193..1206 /label=polya misc_feature 1166..1206 /label=BBa_B0012 terminator 1166..1206 /label=BBa_B0012 stem_loop 1227..1249 /label=stem_loop misc_feature 1215..1260 /label=BBa_B0011 terminator 1215..1260 /label=BBa_B0011 terminator 1166..1260 /label=BBa_B0014 terminator 1166..1260 /label=BBa_K823017 misc_feature 1269..1287 /label=TetR 1 misc_feature 1269..1322 /label=BBa_R0040 promoter 1288..1293 /label=-35 misc_feature 1294..1312 /label=TetR 2 promoter 1311..1316 /label=-10 promoter 1269..1322 /label=BBa_R0040 RBS 1331..1343 /label=RBS-3\Weak RBS 1337..1340 /label=RBS misc_feature 1331..1343 /label=BBa_B0032 RBS 1331..1343 /label=BBa_B0032 misc_feature 1789..1789 /label=g -> a to eliminate PstI site misc_feature 1652..1654 /label=pchA start codon misc_feature 1350..1352 /label=pchB start codon misc_feature 1653..1655 /label=pchB stop codon misc_feature 3080..3085 /label=pchA double TAA stop codon CDS 1350..1655 /label=PchB CDS 1652..3085 /label=PchA CDS 1350..3085 /label=BBa_J45017 stem_loop 3105..3148 /label=stem_loop misc_feature 3094..3173 /label=BBa_B0010 terminator 3094..3173 /label=BBa_B0010 stem_loop 3189..3208 /label=T7 TE misc_feature 3215..3215 /label=stop polyA_site 3209..3222 /label=polya misc_feature 3182..3222 /label=BBa_B0012 terminator 3182..3222 /label=BBa_B0012 terminator 3094..3222 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agattaaaga ggagaaatac tagatggaag ttgttgaagt tcttcacatg aatggaggaa 121 atggagacag tagctatgca aacaattctt tggttcagca aaaggtgatt ctcatgacaa 181 agccaataac tgagcaagcc atgattgatc tctacagcag cctctttcca gaaaccttat 241 gcattgcaga tttgggttgt tctttgggag ctaacacttt cttggtggtc tcacagcttg 301 ttaaaatagt agaaaaagaa cgaaaaaagc atggttttaa gtctccagag ttttattttc 361 acttcaatga tcttcctggc aatgatttta atacactttt tcagtcactg ggggcatttc 421 aagaagattt gagaaagcat ataggggaaa gctttggtcc atgttttttc agtggagtgc 481 ctggttcatt ttatactaga cttttccctt ccaaaagttt acattttgtt tactcctcct 541 acagtctcat gtggctatct caggtgccta atgggattga aaataacaag ggaaacattt 601 acatggcaag aacaagccct ctaagtgtta ttaaagcata ctacaagcaa tatgaaatag 661 atttttcaaa ttttctcaag taccgttcag aggaattgat gaaaggtgga aagatggtgt 721 taacactcct aggtagagaa agtgaggatc ctactagcaa agaatgctgt tacatttggg 781 agcttctagc catggccctc aataagttgg ttgaagaggg attgataaaa gaagagaaag 841 tagatgcatt caatattcct caatacacac catcaccagc agaagtaaag tacatagttg 901 agaaggaagg atcattcacc attaatcgct tggaaacatc aagagttcat tggaatgctt 961 ctaataatga gaagaatggt ggttacaatg tgtcaaggtg catgagagct gtggctgagc 1021 ctttgcttgt cagccacttt gacaaggaat tgatggattt agtgttccac aagtacgaag 1081 agattgtttc tgattgcatg tccaaagaga atactgagtt tataaatgtc atcatctcct 1141 tgaccaaaat aaattaatac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 1201 tttatatact agagagagaa tataaaaagc cagattatta atccggcttt tttattattt 1261 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc 1321 actactagag tcacacagga aagtactaga tgaaaactcc cgaagactgc accggcctgg 1381 cggacatccg cgaggccatc gaccggatcg acctggatat cgtccaggcc ctcggccgcc 1441 gcatggacta cgtcaaggcg gcgtcgcgct tcaaggccag cgaggcggcg attccggcgc 1501 ccgagcgggt cgccgcgatg ctccccgagc gcgcccgctg ggccgaggaa aacggactcg 1561 acgcgccctt cgtcgaggga ctgttcgcgc agatcatcca ctggtacatc gccgagcaga 1621 tcaagtactg gcgccagaca cggggtgccg catgagccgg ctggcgcccc tgagccagtg 1681 cctgcacgcc ttgcgcggca ccttcgagcg cgccatcggc caggcgcagg cgctcgatcg 1741 tccggtgctg gtggcggcat cgttcgagat cgacccattg gacccgctac aggtattcgg 1801 tgcctgggac gaccggcaaa cgccctgcct gtactgggaa cagcccgagc tggcgttctt 1861 cgcctggggc tgcgccctgg agctgcaagg ccacggcgaa cagcgcttcg cccggatcga 1921 ggaaaactgg caattgctct gcgccgacgc cgtggtcgag ggcccgctgg cgccgcgcct 1981 gtgcggcgga ttccgcttcg atccgcgcgg cccgcgcgag gaacactggc aagccttcgc 2041 cgatgccagc ctgatgctcg ccggcatcac cgtgctgcgc gagggcgaac gctaccgggt 2101 actctgccaa cacctggcca agcccggcga agatgccctg gccctggccg cctaccactg 2161 ctcggcgcta ctgcgcctga ggcagccggc cagacgccgg ccctcggggc cgaccgctgg 2221 cgcgcagggc gacgcttcgg cgcaggagcg caggcaatgg gaagccaagg tgagcgacgc 2281 ggtaagcagt gtccgccagg gacgcttcgg caaggtcgtg ctggcccgca cccaggcccg 2341 gcctctcggc gacatcgagc cgtggcaggt catcgaacac ctgcgtctgc aacatgccga 2401 cgcccagctg ttcgcctgtc gccgcggcaa cgcctgcttc ctcggcgcct ccccggaacg 2461 cctggtccgc attcgcgccg gcgaggcact cacccatgcc ctggccggga ccatcgcccg 2521 cggcggcgat gcccaggaag atgcgcggct cggacaggcc ctgctggaca gcgccaagga 2581 caggcacgaa caccagttgg tggtggaggc gatccgtacg gccctggaac ccttcagcga 2641 ggtgctggaa atccccgatg cgcccggcct gaaacgactg gcgcgagtcc agcacctgaa 2701 cacgccgatc cgcgcccgcc tcgctgacgc aggcggcatc ctgcggctgc tacaagcgct 2761 gcatccgacc cccgcggtgg gcggctaccc acgcagcgcg gcgctggact acatccgcca 2821 gcacgaaggg atggaccgcg gctggtacgc cgcgccgctg ggctggctcg acggcgaagg 2881 caacggcgat ttcctggtgg cgctgcgctc ggccctgctc acgccgggcc ggggctacct 2941 gttcgccggc tgcggtctgg taggcgattc ggaaccggcc cacgagtatc gcgaaacctg 3001 ccttaagctc agtgccatgc gggaagctct atccgccata ggcggcctgg acgaagtgcc 3061 cttgcagcgc ggcgtcgcct aataatacta gagccaggca tcaaataaaa cgaaaggctc 3121 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3181 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //