LOCUS BBa_K1060013 2417 bp DNA linear 22-Nov-2024 DEFINITION LacI-AroG* production under regulation of TetR repressible promoter ACCESSION BBa_K1060013 VERSION BBa_K1060013.1 FEATURES Location/Qualifiers misc_feature 1..54 /label=BBa_R0040 misc_feature 61..75 /label=BBa_B0030 misc_feature 82..1234 /label=BBa_C0012 misc_feature 1243..1255 /label=BBa_B0032 CDS 1262..2314 /label=AroG* (mutated) misc_feature 2323..2417 /label=BBa_K823017 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 attaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca 121 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt 181 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac 241 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 301 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg 361 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg 421 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac 481 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc 541 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc 601 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt 661 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 721 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg 781 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg 841 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta 901 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa 961 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 1021 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 1081 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 1141 cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta 1201 gcttaataac tctgatagtg ctagtgtaga tctctactag agtcacacag gaaagtacta 1261 gatgaattat cagaacgacg atttacgcat caaagaaatc aaagagttac ttcctcctgt 1321 cgcattgctg gaaaaattcc ccgctactga aaatgccgcg aatacggttg cccatgcccg 1381 aaaagcgatc cataagatcc tgaaaggtaa tgatgatcgc ctgttggttg tgattggccc 1441 atgctcaatt catgatcctg tcgcggcaaa agagtatgcc actcgcttgc tggcgctgcg 1501 tgaagagctg aaagatgagc tggaaatcgt aatgcgcgtc tattttgaaa agccgcgtac 1561 cacggtgggc tggaaagggc tgattaacga tccgcatatg gataatagct tccagatcaa 1621 cgacggtctg cgtatagccc gtaaattgct gcttgatatt aacgacagcg gtctgccagc 1681 ggcaggtgag tttctcgata tgatcaccct acaatatctc gctgacctga tgagctgggg 1741 cgcaattggc gcacgtacca ccgaatcgca ggtgcaccgc gaagatgcat cagggctttc 1801 ttgtccggtc ggcttcaaaa atggcaccga cggtacgatt aaagtggcta tcgatgccat 1861 taatgccgcc ggtgcgccgc actgcttcct gtccgtaacg aaatgggggc attcggcgat 1921 tgtgaatacc agcggtaacg gcgattgcca tatcattctg cgcggcggta aagagcctaa 1981 ctacagcgcg aagcacgttg ctgaagtgaa agaagggctg aacaaagcag gcctgccagc 2041 acaggtgatg atcgatttca gccatgctaa ctcgtccaaa caattcaaaa agcagatgga 2101 tgtttgtgct gacgtttgcc agcagattgc cggtggcgaa aaggccatta ttggcgtgat 2161 ggtggaaagc catctggtgg aaggcaatca gagcctcgag agcggggagc cgctggccta 2221 cggtaagagc atcaccgatg cctgcatcgg ctgggaagat accgatgctc tgttacgtca 2281 actggcgaat gcagtaaaag cgcgtcgcgg gtaatactag agtcacactg gctcaccttc 2341 gggtgggcct ttctgcgttt atatactaga gagagaatat aaaaagccag attattaatc 2401 cggctttttt attattt //