LOCUS       BBa_K1060014            1923 bp    DNA     linear       22-Dec-2024
DEFINITION  Strong constitutive expression of EBF synthase from Artemisia annua
ACCESSION   BBa_K1060014
VERSION     BBa_K1060014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K608002
     CDS             62..1786
                     /label=BBa_K1060002
     stem_loop       1806..1849
                     /label=stem_loop
     misc_feature    1795..1874
                     /label=BBa_B0010
     terminator      1795..1874
                     /label=BBa_B0010
     stem_loop       1890..1909
                     /label=T7 TE
     misc_feature    1916..1916
                     /label=stop
     polyA_site      1910..1923
                     /label=polya
     misc_feature    1883..1923
                     /label=BBa_B0012
     terminator      1883..1923
                     /label=BBa_B0012
     terminator      1795..1923
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatgtcgact cttcctattt ctagtgtttc attctcttca tctacgtcac ctttggttgt
      121 tgacgataaa gttagcacga aaccagatgt tatccgccat accatgaatt tcaatgctag
      181 catatgggga gatcaatttc ttacatatga tgagccagaa gatttggtta tgaagaaaca
      241 acttgttgaa gagctcaaag aggaagtcaa gaaagagcta ataacgatca aaggttcaaa
      301 tgaaccgatg cagcatgtca agttgataga acttattgat gcagtccaac ggcttggcat
      361 agcctatcat tttgaagagg agattgaaga agccttgcaa catatccatg ttacatatgg
      421 cgagcagtgg gtcgataaag aaaacctaca aagcatttct ctttggttcc gactcctacg
      481 acaacaaggc ttcaacgtct catcaggagt attcaaggac tttatggacg agaagggaaa
      541 atttaaggaa tccttatgta atgatgctca gggcattctt gctctgtatg aagcggcatt
      601 tatgagggtg gaagatgaaa caatactaga taatgcgctc gagttcacta aagttcacct
      661 tgacatcata gccaaggatc cttcttgtga ctcttcccta agaacccaaa tacaccaagc
      721 gttaaagcag ccacttagga gaaggctggc aaggattgag gcgttgcatt acatgcctat
      781 ctaccaacaa gaaacatccc acgatgaggt cttactgaag cttgcaaaat tagattttag
      841 cgtgcttcag tcgatgcaca aaaaggaact tagccacatt tgcaaatggt ggaaggattt
      901 ggacctccaa aataagcttc cttatgttcg agacagggtg gtggaaggct atttttggat
      961 actatccatc tattacgagc ctcaacatgc tcgaacaaga atgttcctaa tgaaaacatg
     1021 catgtggtta gtcgttttag atgatacatt tgataattat ggtacttatg aagaactcga
     1081 aatctttaca caagctgttg aaagatggtc aataagttgc ctggatatgc ttccagaata
     1141 catgaaacta atatatcaag agcttgtgaa tcttcacgtg gaaatggagg aatcacttga
     1201 aaaggaggga aaaacatatc aaattcacta tgtcaaggag atggcaaaag agttggttcg
     1261 caattacttg gtagaagcca gatggctaaa agaggggtac atgccaactc ttgaggagta
     1321 catgtctgtg tcaatggtga ctggtaccta tggcttgatg atagcgagat cttatgtcgg
     1381 caggggtgat atcgtcaccg aggatacctt taaatgggtg tcctcgtatc ctcctattat
     1441 aaaagcttca tgtgtgattg taagacttat ggatgatatt gtcagccaca aggaggaaca
     1501 agagagaggc catgttgctt caagcatcga atgctattct aaggaaagtg gtgcatcaga
     1561 ggaggaagcg tgtgaatata tctcaagaaa agttgaagat gcatggaaag ctataaaccg
     1621 agagtcgctc aggcctacag ctgtcccatt tcctctactt atgcctgcaa tcaaccttgc
     1681 acgtatgtgt gaagtcctat atagcgtcaa cgatggcttc actcatgctg agggagacat
     1741 gaaaagttac atgaaatcgt tcttcgttca ccctatggtt gtctaatact agagccaggc
     1801 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1861 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1921 ata
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