BBa_J23104 1 BBa_J23104 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z isolated from library of promoters replace later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K608002 1 BBa_K608002 strong Promoter and strong RBS 2011-09-14T11:00:00Z 2015-05-08T01:12:52Z assembly from iGEM parts Released HQ 2013 you can insert your part behind this part so it will be immediatly expressed in e.coli. false false _780_ 0 9115 9 In stock false cloning via 3a-assembly false Julia M??ller component2128648 1 BBa_B0034 component2128646 1 BBa_J23104 annotation2128646 1 BBa_J23104 range2128646 1 1 35 annotation2128648 1 BBa_B0034 range2128648 1 44 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K1060014 1 BBa_K1060014 Strong constitutive expression of EBF synthase from Artemisia annua 2013-09-29T11:00:00Z 2015-05-08T01:08:58Z iGEM bricks: BBa_K608002, Bba_K1060002, Bba_B0015 For the production of the alarmhormone,(E)-&#946;-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a strong (constitutive) promoter and strong RBS (<partinfo>BBa_K608002</partinfo>). false false _1368_ 0 17634 9 Not in stock false no EcoRI, XbaI, NotI, SpeI or PstI sites present false Ingmar Claes, Sylvie Weckx, Su Wang component2366780 1 BBa_K608002 component2366788 1 BBa_B0015 component2366781 1 BBa_K1060002 annotation2366780 1 BBa_K608002 range2366780 1 1 55 annotation2366788 1 BBa_B0015 range2366788 1 1795 1923 annotation2366781 1 BBa_K1060002 range2366781 1 62 1786 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1060002 1 EbfS Aa EBF synthase from Artemisia annua 2013-09-16T11:00:00Z 2015-05-08T01:08:57Z Artemisia annua genomic DNA (Picaud et al. 2005) This sesquiterpene synthase (GenBank Accession No. AY835398), (E)-b-farnesene synthase, has been isolated from Artemisia annua L (Picaud et al. 2005) false false _1368_ 0 17634 9 In stock true An EcoRI site (513A>C)was removed by site directed mutagenesis false Ingmar Claes, Lukas Marcelis, Su Wang BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1060014_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgtcgactcttcctatttctagtgtttcattctcttcatctacgtcacctttggttgttgacgataaagttagcacgaaaccagatgttatccgccataccatgaatttcaatgctagcatatggggagatcaatttcttacatatgatgagccagaagatttggttatgaagaaacaacttgttgaagagctcaaagaggaagtcaagaaagagctaataacgatcaaaggttcaaatgaaccgatgcagcatgtcaagttgatagaacttattgatgcagtccaacggcttggcatagcctatcattttgaagaggagattgaagaagccttgcaacatatccatgttacatatggcgagcagtgggtcgataaagaaaacctacaaagcatttctctttggttccgactcctacgacaacaaggcttcaacgtctcatcaggagtattcaaggactttatggacgagaagggaaaatttaaggaatccttatgtaatgatgctcagggcattcttgctctgtatgaagcggcatttatgagggtggaagatgaaacaatactagataatgcgctcgagttcactaaagttcaccttgacatcatagccaaggatccttcttgtgactcttccctaagaacccaaatacaccaagcgttaaagcagccacttaggagaaggctggcaaggattgaggcgttgcattacatgcctatctaccaacaagaaacatcccacgatgaggtcttactgaagcttgcaaaattagattttagcgtgcttcagtcgatgcacaaaaaggaacttagccacatttgcaaatggtggaaggatttggacctccaaaataagcttccttatgttcgagacagggtggtggaaggctatttttggatactatccatctattacgagcctcaacatgctcgaacaagaatgttcctaatgaaaacatgcatgtggttagtcgttttagatgatacatttgataattatggtacttatgaagaactcgaaatctttacacaagctgttgaaagatggtcaataagttgcctggatatgcttccagaatacatgaaactaatatatcaagagcttgtgaatcttcacgtggaaatggaggaatcacttgaaaaggagggaaaaacatatcaaattcactatgtcaaggagatggcaaaagagttggttcgcaattacttggtagaagccagatggctaaaagaggggtacatgccaactcttgaggagtacatgtctgtgtcaatggtgactggtacctatggcttgatgatagcgagatcttatgtcggcaggggtgatatcgtcaccgaggatacctttaaatgggtgtcctcgtatcctcctattataaaagcttcatgtgtgattgtaagacttatggatgatattgtcagccacaaggaggaacaagagagaggccatgttgcttcaagcatcgaatgctattctaaggaaagtggtgcatcagaggaggaagcgtgtgaatatatctcaagaaaagttgaagatgcatggaaagctataaaccgagagtcgctcaggcctacagctgtcccatttcctctacttatgcctgcaatcaaccttgcacgtatgtgtgaagtcctatatagcgtcaacgatggcttcactcatgctgagggagacatgaaaagttacatgaaatcgttcttcgttcaccctatggttgtctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K1060002_sequence 1 atgtcgactcttcctatttctagtgtttcattctcttcatctacgtcacctttggttgttgacgataaagttagcacgaaaccagatgttatccgccataccatgaatttcaatgctagcatatggggagatcaatttcttacatatgatgagccagaagatttggttatgaagaaacaacttgttgaagagctcaaagaggaagtcaagaaagagctaataacgatcaaaggttcaaatgaaccgatgcagcatgtcaagttgatagaacttattgatgcagtccaacggcttggcatagcctatcattttgaagaggagattgaagaagccttgcaacatatccatgttacatatggcgagcagtgggtcgataaagaaaacctacaaagcatttctctttggttccgactcctacgacaacaaggcttcaacgtctcatcaggagtattcaaggactttatggacgagaagggaaaatttaaggaatccttatgtaatgatgctcagggcattcttgctctgtatgaagcggcatttatgagggtggaagatgaaacaatactagataatgcgctcgagttcactaaagttcaccttgacatcatagccaaggatccttcttgtgactcttccctaagaacccaaatacaccaagcgttaaagcagccacttaggagaaggctggcaaggattgaggcgttgcattacatgcctatctaccaacaagaaacatcccacgatgaggtcttactgaagcttgcaaaattagattttagcgtgcttcagtcgatgcacaaaaaggaacttagccacatttgcaaatggtggaaggatttggacctccaaaataagcttccttatgttcgagacagggtggtggaaggctatttttggatactatccatctattacgagcctcaacatgctcgaacaagaatgttcctaatgaaaacatgcatgtggttagtcgttttagatgatacatttgataattatggtacttatgaagaactcgaaatctttacacaagctgttgaaagatggtcaataagttgcctggatatgcttccagaatacatgaaactaatatatcaagagcttgtgaatcttcacgtggaaatggaggaatcacttgaaaaggagggaaaaacatatcaaattcactatgtcaaggagatggcaaaagagttggttcgcaattacttggtagaagccagatggctaaaagaggggtacatgccaactcttgaggagtacatgtctgtgtcaatggtgactggtacctatggcttgatgatagcgagatcttatgtcggcaggggtgatatcgtcaccgaggatacctttaaatgggtgtcctcgtatcctcctattataaaagcttcatgtgtgattgtaagacttatggatgatattgtcagccacaaggaggaacaagagagaggccatgttgcttcaagcatcgaatgctattctaaggaaagtggtgcatcagaggaggaagcgtgtgaatatatctcaagaaaagttgaagatgcatggaaagctataaaccgagagtcgctcaggcctacagctgtcccatttcctctacttatgcctgcaatcaaccttgcacgtatgtgtgaagtcctatatagcgtcaacgatggcttcactcatgctgagggagacatgaaaagttacatgaaatcgttcttcgttcaccctatggttgtctaa BBa_K608002_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa BBa_J23104_sequence 1 ttgacagctagctcagtcctaggtattgtgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z