BBa_K1061006 1 BBa_K1061006 Bax mutant form 2013-09-11T11:00:00Z 2015-05-08T01:08:58Z We clone it from the plasmid provided by addgene. Hbax, a member of Bcl-2 family, is an apoptosis-related protein. It integrates into the mitochondrial outer membrane, releases cytochrome C and leads to apoptosis.Hbax184a is a mutant of hbax that constantly integrates into mitochondrial outer membrane. We have tried to use them as suicide genes, but they can not induce apoptosis in most cancer cell lines, probably because the pathway that they involve has been blocked in most cancer cells. However, we find that the protein can induce ???apoptosis??? in the most broadly used eukaryotic chassis, yeast. So it will be very potential to use the gene in some circuit that need to kill the yeast in proper condition.We submit this part as the improvement of hbax that already in the part registry. false false _1369_ 0 11892 9 It's complicated false Although it is not toxic to most cancer cells, unfortunately, it is toxic to normal cells. So it is not suggested to use it in the design of mammalian circuit. But it will be useful in constructing yeast circuit as we mentioned. false mengyi sun BBa_K1061006_sequence 1 atggacgggtccggggagcagcccagaggcggggggcccaccagctctgagcagatcatgaagacaggggcccttttgcttcagggtttcatccaggatcgagcagggcgaatggggggggaggcacccgagctggccctggacccggtgcctcaggatgcgtccaccaagaagctgagcgagtgtctcaagcgcatcggggacgaactggacagtaacatggagctgcaaaggatgattgccgccgtggacacagactccccccgagaggtctttttccgagtggcagctgacatgttttctgacggcaacttcaactggggccgggttgtcgcccttttctactttgccagcaaactggtgctcaaggccctgtgcaccaaggtgccggaactgatcagaaccatcatgggctggacattggacttcctccgggagcggctgttgggctggatccaagaccagggtggttgggacggcctcctctcctactttgggacgcccacgtggcagaccgtgaccatctttgtggcgggagtgctcaccgccgcgctcaccatctggaagaaaatgggctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z