BBa_K1061007
1
BBa_K1061007
Bax 184a
2013-09-12T11:00:00Z
2015-05-08T01:08:58Z
We get the parts from addgene. The plasmids are originally construct by Youle???s lab.
Hbax, a member of Bcl-2 family, is an apoptosis-related protein. It integrates into the mitochondrial outer membrane, releases cytochrome C and leads to apoptosis.Hbax184a is a mutant of hbax that constantly integrates into mitochondrial outer membrane. We have tried to use them as suicide genes, but they can not induce apoptosis in most cancer cell lines, probably because the pathway that they involve has been blocked in most cancer cells. However, we find that the protein can induce ???apoptosis??? in the most broadly used eukaryotic chassis, yeast. So it will be very potential to use the gene in some circuit that need to kill the yeast in proper condition.
false
false
_1369_
0
11892
9
No part sequence
false
Although it is not toxic to most cancer cells, unfortunately, it maybe toxic to normal cells. So it is not suggested to use it in the design of mammalian circuit. But it will be useful in constructing yeast circuit as we mentioned.
false
mengyi sun
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z