BBa_K1061001
1
BBa_K1061001
apoptin
2013-09-10T11:00:00Z
2015-05-08T01:08:58Z
Cloning from plasmid from Zhuang's lab.
Apoptin is a protein that is originally isolated from the chicken anemia virus(CAV). It has been found that expressing this gene can induce apoptosis in a broad spectrum of cancer cells but not in normal cells. Hence it is a very powerful tool in treating cancer. Fusion the TAT protein with apoptin can even achieve by-stander effect, and we try to use it in the most simple device of our project. We have successfully expressed it in Hep G2 cell lines and observe a brilliant apoptosis effect.
false
false
_1369_
0
11892
9
In stock
false
Although it has been try in a broad spectrum of cells and prove to be safe to the normal cells, it still has not been tried in ALL normal cells. So before using it to treat certain kinds of cancer, every team need to ensure that it want hurt the specify normal cells involve in the project. And it is a protein contain 121 amino acid that may big enough to induce immune reaction(although in the experiment of mouse it has not been reported.)
false
mengyi sun
annotation2362082
1
NLS 1
range2362082
1
244
264
annotation2339023
1
leucine rich fragment
range2339023
1
97
138
BBa_K1061021
1
BBa_K1061021
EF-1alpha-apoptin
2013-09-16T11:00:00Z
2015-05-08T01:08:58Z
We got the apoptin from Zhuang's lab,and cloned the EF-1alpha promoter from the plasmid provided by Lei Xiao.
This device is a generator that constitutively express apoptin, a protein that can speficially induce apoptosis in cancer cells but not in normal cells. So that it can work like a general safeguard device for the protection of gene therapy.
false
false
_1369_
0
11892
9
Not in stock
false
Although it has been try in a broad spectrum of cells and prove to be safe to the normal cells, it still has not been tried in ALL normal cells. So before using it to treat certain kinds of cancer, every team need to ensure that it want hurt the specify normal cells involve in the project. And it is a protein contain 121 amino acid that may big enough to induce immune reaction(although in the experiment of mouse it has not been reported.)
false
mengyi sun
component2348973
1
BBa_K1061011
component2348974
1
BBa_K1061001
annotation2348974
1
BBa_K1061001
range2348974
1
544
909
annotation2348973
1
BBa_K1061011
range2348973
1
1
537
BBa_K1061011
1
BBa_K1061011
EF-1alpha promoter
2013-09-10T11:00:00Z
2015-05-08T01:08:58Z
We clone this part from the plasmid provided by Lei Xiao's lab.
Ef-1alpha promoter is a strong constitutive promoter that drive the expression of elongation factor -1α.It has been widely used in constructing mammalian cell-based circuit, especially useful for construction of long-term circuit in some cells including iPS cells and Jurkat cells in which the strong promoter CMV may be silenced.
false
false
_1369_
0
11892
9
In stock
false
The promoter that we submit comes from the lentivector constructed by Lei Xiao???s lab. It is a truncated version of the full-length human EF-1α promoter, including a cPPT/CTS fragment which can enhance the transduction efficiency of lentivectors.
false
mengyi sun
BBa_K1061001_sequence
1
atgaacgctctccaagaagatactccacccggaccatcaacggtgttcaggccaccaacaagttcacggccgttggaaacccctcactgccgagagatccggattggtatcgctggaattacaatcactctatcgctgtgtggctgcgcgaatgctcgcgctcccacgctaagatctgcaactgcggacaattcagaaagcactggtttcaagaatgtgccggacttgaggaccgatcaacccaagcctccctcgaagaagcgatcctgcgacccctccgagtacagggtaagcgagctaaaagaaagcttgattaccactactcccagccgaccccgaaccgcaaaaaggcgtataagactgtaa
BBa_K1061021_sequence
1
ttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgatcacgagactagcctcgacacaaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagaaatccactttggctcgagaagcttgatatcggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtactagatgaacgctctccaagaagatactccacccggaccatcaacggtgttcaggccaccaacaagttcacggccgttggaaacccctcactgccgagagatccggattggtatcgctggaattacaatcactctatcgctgtgtggctgcgcgaatgctcgcgctcccacgctaagatctgcaactgcggacaattcagaaagcactggtttcaagaatgtgccggacttgaggaccgatcaacccaagcctccctcgaagaagcgatcctgcgacccctccgagtacagggtaagcgagctaaaagaaagcttgattaccactactcccagccgaccccgaaccgcaaaaaggcgtataagactgtaa
BBa_K1061011_sequence
1
ttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgatcacgagactagcctcgacacaaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagaaatccactttggctcgagaagcttgatatcggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z