LOCUS       BBa_K1064000            1530 bp    DNA     linear       13-Jan-2025
DEFINITION  constitutive promoter+strong RBS+cyp3A4+double terminator
ACCESSION   BBa_K1064000
VERSION     BBa_K1064000.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..1483
                     /label=BBa_K1064004
     misc_feature    1492..1530
                     /label=B1006
     stem_loop       1501..1522
                     /label=modified thr terminator
     polyA_site      1523..1530
                     /label=PolyA
     polyA_site      1492..1500
                     /label=PolyA
     terminator      1492..1530
                     /label=BBa_B1006
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagattaaag aggagaaata
       61 ctagatgttt aagaagcttg ggattccagg gcccacacct ctgccttttt tgggaaatat
      121 tttgtcctac cataagggct tttgtatgtt tgacatggaa tgtcataaaa agtatggaaa
      181 agtgtggggc ttttatgatg gtcaacagcc tgtgctggct atcacagatc ctgacatgat
      241 caaaacagtg ctagtgaaag aatgttattc tgtcttcaca aaccggaggc cttttggtcc
      301 agtgggattt atgaaaagtg ccatctctat agctgaggat gaagaatgga agagattacg
      361 atcattgctg tctccaacct tcaccagtgg aaaactcaag gagatggtcc ctatcattgc
      421 ccagtatgga gatgtgttgg tgagaaatct gaggcgggaa gcagagacag gcaagcctgt
      481 caccttgaaa gacgtctttg gggcctacag catggatgtg atcactagca catcatttgg
      541 agtgaacatc gactctctca acaatccaca agaccccttt gtggaaaaca ccaagaagct
      601 tttaagattt gattttttgg atccattctt tctctcaata acagtctttc cattcctcat
      661 cccaattctt gaagtattaa atatctgtgt gtttccaaga gaagttacaa attttttaag
      721 aaaatctgta aaaaggatga aagaaagtcg cctcgaagat acacaaaagc accgagtgga
      781 tttccttcag ctgatgattg actctcaaaa ttcaaaagaa actgagtccc acaaagctct
      841 gtccgatctg gagctcgtgg cccaatcaat tatctttatt tttgctggct atgaaaccac
      901 gagcagtgtt ctctccttca ttatgtatga actggccact caccctgatg tccagcagaa
      961 actgcaagag gaaattgatg cagttttacc caataaggca ccacccacct atgatactgt
     1021 gctacagatg gagtatcttg acatggtggt gaatgaaacg ctcagattat tcccaattgc
     1081 tatgagactt gagagggtct gcaaaaaaga tgttgagatc aatgggatgt tcattcccaa
     1141 aggggtggtg gtgatgattc caagctatgc tcttcaccgt gacccaaagt actggacaga
     1201 gcctgagaag ttcctccctg aaagattcag caagaagaac aaggacaaca tagatcctta
     1261 catatacaca ccctttggaa gtggacccag aaactgcatt ggcatgaggt ttgctctcat
     1321 gaacatgaaa cttgctctaa tcagagtcct tcagaacttc tccttcaaac cttgtaaaga
     1381 aacacagatc cccctgaaat taagcttagg aggacttctt caaccagaaa aacccgttgt
     1441 tctaaaggtt gagtcaaggg atggcaccgt aagtggagcc tgatactaga gaaaaaaaaa
     1501 ccccgcccct gacagggcgg ggtttttttt
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