LOCUS       BBa_K1065001            2426 bp    DNA     linear       26-Nov-2024
DEFINITION  AraC-pBAD + 2-oxoglutarate oxygenase/decarboxylase (EFE) + 
            terminators
ACCESSION   BBa_K1065001
VERSION     BBa_K1065001.1  
FEATURES             Location/Qualifiers
     promoter        1080..1203
                     /label=pBAD
     promoter        1043..1114
                     /label=PC
     CDS             1..1080
                     /label=araC
     promoter        1..1203
                     /label=BBa_K731201
     RBS             1212..1217
                     /label=RBS
     misc_feature    1212..2289
                     /label=BBa_K1065000
     misc_feature    1218..1224
                     /label=Spacer
     CDS             1225..2289
                     /label=CDS - Ethylene Forming Enzyme
     misc_feature    1225..1227
                     /label=Start
     misc_feature    1228..1233
                     /label=NgoMIV 
     misc_feature    2281..2286
                     /label=AgeI
     misc_feature    2287..2289
                     /label=Stop
     misc_feature    1212..2289
                     /label=BBa_K1065000
     stem_loop       2309..2352
                     /label=stem_loop
     misc_feature    2298..2377
                     /label=BBa_B0010
     terminator      2298..2377
                     /label=BBa_B0010
     stem_loop       2393..2412
                     /label=T7 TE
     misc_feature    2419..2419
                     /label=stop
     polyA_site      2413..2426
                     /label=polya
     misc_feature    2386..2426
                     /label=BBa_B0012
     terminator      2386..2426
                     /label=BBa_B0012
     terminator      2298..2426
                     /label=BBa_B0015
     misc_feature    1212..2426
                     /label=BBa_K1065002
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg
      181 actaatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgtactaga gaggaggaac tactatggcc ggcaccaacc tgcaaacatt tgaactgccg
     1261 acggaagtaa ctggctgcgc tgcggacatt tcgctgggac gtgccctgat ccaagcatgg
     1321 cagaaagatg gcatttttca aatcaaaacc gattcggaac aggaccgtaa aacacaagaa
     1381 gccatggcgg catctaaaca gttctgcaaa gaaccgctga cgtttaaaag ctcatgtgtc
     1441 agcgatctga cttattcagg atacgtggcg tctggcgaag aagttacggc aggcaaaccg
     1501 gactttccgg aaatcttcac tgtgtgcaaa gatctgtcag tcggtgacca gagagtaaaa
     1561 gctggttggc cgtgtcatgg cccggtgccg tggccgaaca acacctacca aaaaagcatg
     1621 aaaacattca tggaagaact gggcctggcc ggtgaacgcc tgctgaaact gacggctctg
     1681 ggtttcgaac tgccgattaa tacgtttact gatctgaccc gcgacggatg gcatcacatg
     1741 cgtgtgctga gatttccgcc gcagaccagt acactgtcgc gtggtattgg agcgcatacc
     1801 gattatggac tgctggtcat cgccgctcaa gatgacgtag gcggtctgta cattcgtccg
     1861 ccggtcgaag gtgaaaaacg caatcgtaac tggctgccgg gcgaatcttc cgcaggtatg
     1921 ttcgaacacg atgaaccgtg gacctttgta acgccgactc cgggagtgtg gacagttttc
     1981 ccgggcgaca tcctgcaatt tatgacggga ggccaactgc tgtccactcc gcataaagtt
     2041 aaactgaata caagagaacg ctttgcgtgc gcatatttcc acgaaccgaa ctttgaagcc
     2101 tccgcttacc cgctgtttga accgagtgca aatgaacgca ttcattatgg cgaacacttt
     2161 acgaacatgt tcatgcgctg ttacccggat cgtattacca cacaaagaat caacaaagaa
     2221 aatagactgg cacacctgga agacctgaaa aaatactcgg acacaagagc aactggttcg
     2281 accggttaat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2341 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2401 ttcgggtggg cctttctgcg tttata
//