LOCUS       BBa_K1065002            1215 bp    DNA     linear       22-Dec-2024
DEFINITION  EFE + Terminators
ACCESSION   BBa_K1065002
VERSION     BBa_K1065002.1  
FEATURES             Location/Qualifiers
     RBS             1..6
                     /label=RBS
     misc_feature    1..1078
                     /label=BBa_K1065000
     misc_feature    7..13
                     /label=Spacer
     CDS             14..1078
                     /label=CDS - Ethylene Forming Enzyme
     misc_feature    14..16
                     /label=Start
     misc_feature    17..22
                     /label=NgoMIV 
     misc_feature    1070..1075
                     /label=AgeI
     misc_feature    1076..1078
                     /label=Stop
     misc_feature    1..1078
                     /label=BBa_K1065000
     stem_loop       1098..1141
                     /label=stem_loop
     misc_feature    1087..1166
                     /label=BBa_B0010
     terminator      1087..1166
                     /label=BBa_B0010
     stem_loop       1182..1201
                     /label=T7 TE
     misc_feature    1208..1208
                     /label=stop
     polyA_site      1202..1215
                     /label=polya
     misc_feature    1175..1215
                     /label=BBa_B0012
     terminator      1175..1215
                     /label=BBa_B0012
     terminator      1087..1215
                     /label=BBa_B0015
ORIGIN
        1 aggaggaact actatggccg gcaccaacct gcaaacattt gaactgccga cggaagtaac
       61 tggctgcgct gcggacattt cgctgggacg tgccctgatc caagcatggc agaaagatgg
      121 catttttcaa atcaaaaccg attcggaaca ggaccgtaaa acacaagaag ccatggcggc
      181 atctaaacag ttctgcaaag aaccgctgac gtttaaaagc tcatgtgtca gcgatctgac
      241 ttattcagga tacgtggcgt ctggcgaaga agttacggca ggcaaaccgg actttccgga
      301 aatcttcact gtgtgcaaag atctgtcagt cggtgaccag agagtaaaag ctggttggcc
      361 gtgtcatggc ccggtgccgt ggccgaacaa cacctaccaa aaaagcatga aaacattcat
      421 ggaagaactg ggcctggccg gtgaacgcct gctgaaactg acggctctgg gtttcgaact
      481 gccgattaat acgtttactg atctgacccg cgacggatgg catcacatgc gtgtgctgag
      541 atttccgccg cagaccagta cactgtcgcg tggtattgga gcgcataccg attatggact
      601 gctggtcatc gccgctcaag atgacgtagg cggtctgtac attcgtccgc cggtcgaagg
      661 tgaaaaacgc aatcgtaact ggctgccggg cgaatcttcc gcaggtatgt tcgaacacga
      721 tgaaccgtgg acctttgtaa cgccgactcc gggagtgtgg acagttttcc cgggcgacat
      781 cctgcaattt atgacgggag gccaactgct gtccactccg cataaagtta aactgaatac
      841 aagagaacgc tttgcgtgcg catatttcca cgaaccgaac tttgaagcct ccgcttaccc
      901 gctgtttgaa ccgagtgcaa atgaacgcat tcattatggc gaacacttta cgaacatgtt
      961 catgcgctgt tacccggatc gtattaccac acaaagaatc aacaaagaaa atagactggc
     1021 acacctggaa gacctgaaaa aatactcgga cacaagagca actggttcga ccggttaata
     1081 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1141 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1201 ctttctgcgt ttata
//