LOCUS       BBa_K1065101            2441 bp    DNA     linear       18-Jun-2024
DEFINITION  AraCpBAD + BSMT1
ACCESSION   BBa_K1065101
VERSION     BBa_K1065101.1  
FEATURES             Location/Qualifiers
     promoter        1080..1203
                     /label=pBAD
     promoter        1043..1114
                     /label=PC
     CDS             1..1080
                     /label=araC
     promoter        1..1203
                     /label=BBa_K731201
     RBS             1212..1224
                     /label=RBS-3\Weak
     RBS             1218..1221
                     /label=RBS
     misc_feature    1212..1224
                     /label=BBa_B0032
     RBS             1212..1224
                     /label=BBa_B0032
     CDS             1231..2304
                     /label=BMST1
     misc_feature    1231..1233
                     /label=start codon
     misc_feature    2302..2304
                     /label=stop codon
     CDS             1231..2304
                     /label=BBa_J45004
     stem_loop       2324..2367
                     /label=stem_loop
     misc_feature    2313..2392
                     /label=BBa_B0010
     terminator      2313..2392
                     /label=BBa_B0010
     stem_loop       2408..2427
                     /label=T7 TE
     misc_feature    2434..2434
                     /label=stop
     polyA_site      2428..2441
                     /label=polya
     misc_feature    2401..2441
                     /label=BBa_B0012
     terminator      2401..2441
                     /label=BBa_B0012
     misc_feature    1212..2441
                     /label=BBa_J45119
     misc_feature    1212..2441
                     /label=BBa_K1065100
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg
      181 actaatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgtactaga gtcacacagg aaagtactag atggaagttg ttgaagttct tcacatgaat
     1261 ggaggaaatg gagacagtag ctatgcaaac aattctttgg ttcagcaaaa ggtgattctc
     1321 atgacaaagc caataactga gcaagccatg attgatctct acagcagcct ctttccagaa
     1381 accttatgca ttgcagattt gggttgttct ttgggagcta acactttctt ggtggtctca
     1441 cagcttgtta aaatagtaga aaaagaacga aaaaagcatg gttttaagtc tccagagttt
     1501 tattttcact tcaatgatct tcctggcaat gattttaata cactttttca gtcactgggg
     1561 gcatttcaag aagatttgag aaagcatata ggggaaagct ttggtccatg ttttttcagt
     1621 ggagtgcctg gttcatttta tactagactt ttcccttcca aaagtttaca ttttgtttac
     1681 tcctcctaca gtctcatgtg gctatctcag gtgcctaatg ggattgaaaa taacaaggga
     1741 aacatttaca tggcaagaac aagccctcta agtgttatta aagcatacta caagcaatat
     1801 gaaatagatt tttcaaattt tctcaagtac cgttcagagg aattgatgaa aggtggaaag
     1861 atggtgttaa cactcctagg tagagaaagt gaggatccta ctagcaaaga atgctgttac
     1921 atttgggagc ttctagccat ggccctcaat aagttggttg aagagggatt gataaaagaa
     1981 gagaaagtag atgcattcaa tattcctcaa tacacaccat caccagcaga agtaaagtac
     2041 atagttgaga aggaaggatc attcaccatt aatcgcttgg aaacatcaag agttcattgg
     2101 aatgcttcta ataatgagaa gaatggtggt tacaatgtgt caaggtgcat gagagctgtg
     2161 gctgagcctt tgcttgtcag ccactttgac aaggaattga tggatttagt gttccacaag
     2221 tacgaagaga ttgtttctga ttgcatgtcc aaagagaata ctgagtttat aaatgtcatc
     2281 atctccttga ccaaaataaa ttaatactag agccaggcat caaataaaac gaaaggctca
     2341 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2401 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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