LOCUS BBa_K1065102 4404 bp DNA linear 01-Jun-2024 DEFINITION Wintergreen device: Plac + PchBA + AraCpBAD + BSMT1 ACCESSION BBa_K1065102 VERSION BBa_K1065102.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..76 /label=RBS-3\Weak RBS 70..73 /label=RBS misc_feature 64..76 /label=BBa_B0032 RBS 64..76 /label=BBa_B0032 misc_feature 522..522 /label=g -> a to eliminate PstI site misc_feature 385..387 /label=pchA start codon misc_feature 83..85 /label=pchB start codon misc_feature 386..388 /label=pchB stop codon misc_feature 1813..1818 /label=pchA double TAA stop codon CDS 83..388 /label=PchB CDS 385..1818 /label=PchA CDS 83..1818 /label=BBa_J45017 stem_loop 1838..1881 /label=stem_loop misc_feature 1827..1906 /label=BBa_B0010 terminator 1827..1906 /label=BBa_B0010 stem_loop 1922..1941 /label=T7 TE misc_feature 1948..1948 /label=stop polyA_site 1942..1955 /label=polya misc_feature 1915..1955 /label=BBa_B0012 terminator 1915..1955 /label=BBa_B0012 misc_feature 1..1955 /label=BBa_J45320 promoter 3043..3166 /label=pBAD promoter 3006..3077 /label=PC CDS 1964..3043 /label=araC promoter 1964..3166 /label=BBa_K731201 RBS 3175..3187 /label=RBS-3\Weak RBS 3181..3184 /label=RBS misc_feature 3175..3187 /label=BBa_B0032 RBS 3175..3187 /label=BBa_B0032 CDS 3194..4267 /label=BMST1 misc_feature 3194..3196 /label=start codon misc_feature 4265..4267 /label=stop codon CDS 3194..4267 /label=BBa_J45004 stem_loop 4287..4330 /label=stem_loop misc_feature 4276..4355 /label=BBa_B0010 terminator 4276..4355 /label=BBa_B0010 stem_loop 4371..4390 /label=T7 TE misc_feature 4397..4397 /label=stop polyA_site 4391..4404 /label=polya misc_feature 4364..4404 /label=BBa_B0012 terminator 4364..4404 /label=BBa_B0012 misc_feature 3175..4404 /label=BBa_J45119 misc_feature 3175..4404 /label=BBa_K1065100 misc_feature 1964..4404 /label=BBa_K1065101 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagtcacaca ggaaagtact agatgaaaac tcccgaagac tgcaccggcc tggcggacat 121 ccgcgaggcc atcgaccgga tcgacctgga tatcgtccag gccctcggcc gccgcatgga 181 ctacgtcaag gcggcgtcgc gcttcaaggc cagcgaggcg gcgattccgg cgcccgagcg 241 ggtcgccgcg atgctccccg agcgcgcccg ctgggccgag gaaaacggac tcgacgcgcc 301 cttcgtcgag ggactgttcg cgcagatcat ccactggtac atcgccgagc agatcaagta 361 ctggcgccag acacggggtg ccgcatgagc cggctggcgc ccctgagcca gtgcctgcac 421 gccttgcgcg gcaccttcga gcgcgccatc ggccaggcgc aggcgctcga tcgtccggtg 481 ctggtggcgg catcgttcga gatcgaccca ttggacccgc tacaggtatt cggtgcctgg 541 gacgaccggc aaacgccctg cctgtactgg gaacagcccg agctggcgtt cttcgcctgg 601 ggctgcgccc tggagctgca aggccacggc gaacagcgct tcgcccggat cgaggaaaac 661 tggcaattgc tctgcgccga cgccgtggtc gagggcccgc tggcgccgcg cctgtgcggc 721 ggattccgct tcgatccgcg cggcccgcgc gaggaacact ggcaagcctt cgccgatgcc 781 agcctgatgc tcgccggcat caccgtgctg cgcgagggcg aacgctaccg ggtactctgc 841 caacacctgg ccaagcccgg cgaagatgcc ctggccctgg ccgcctacca ctgctcggcg 901 ctactgcgcc tgaggcagcc ggccagacgc cggccctcgg ggccgaccgc tggcgcgcag 961 ggcgacgctt cggcgcagga gcgcaggcaa tgggaagcca aggtgagcga cgcggtaagc 1021 agtgtccgcc agggacgctt cggcaaggtc gtgctggccc gcacccaggc ccggcctctc 1081 ggcgacatcg agccgtggca ggtcatcgaa cacctgcgtc tgcaacatgc cgacgcccag 1141 ctgttcgcct gtcgccgcgg caacgcctgc ttcctcggcg cctccccgga acgcctggtc 1201 cgcattcgcg ccggcgaggc actcacccat gccctggccg ggaccatcgc ccgcggcggc 1261 gatgcccagg aagatgcgcg gctcggacag gccctgctgg acagcgccaa ggacaggcac 1321 gaacaccagt tggtggtgga ggcgatccgt acggccctgg aacccttcag cgaggtgctg 1381 gaaatccccg atgcgcccgg cctgaaacga ctggcgcgag tccagcacct gaacacgccg 1441 atccgcgccc gcctcgctga cgcaggcggc atcctgcggc tgctacaagc gctgcatccg 1501 acccccgcgg tgggcggcta cccacgcagc gcggcgctgg actacatccg ccagcacgaa 1561 gggatggacc gcggctggta cgccgcgccg ctgggctggc tcgacggcga aggcaacggc 1621 gatttcctgg tggcgctgcg ctcggccctg ctcacgccgg gccggggcta cctgttcgcc 1681 ggctgcggtc tggtaggcga ttcggaaccg gcccacgagt atcgcgaaac ctgccttaag 1741 ctcagtgcca tgcgggaagc tctatccgcc ataggcggcc tggacgaagt gcccttgcag 1801 cgcggcgtcg cctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 1861 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 1921 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttatgac aacttgacgg 1981 ctacatcatt cactttttct tcacaaccgg cacgaaactc gctcgggctg gccccggtgc 2041 attttttaaa tactcgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg 2101 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct 2161 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg 2221 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat 2281 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt 2341 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg 2401 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg 2461 gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc aaatattgac ggccagttaa 2521 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag 2581 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg 2641 gtcggcagac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat 2701 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg 2761 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg 2821 gatcattttg cgcttcagcc atacttttca tactcccacc attcagagaa gaaaccaatt 2881 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc 2941 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa 3001 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg 3061 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac 3121 gctttttatc gcaactctct actgtttctc catacccgtt tttttgtact agagtcacac 3181 aggaaagtac tagatggaag ttgttgaagt tcttcacatg aatggaggaa atggagacag 3241 tagctatgca aacaattctt tggttcagca aaaggtgatt ctcatgacaa agccaataac 3301 tgagcaagcc atgattgatc tctacagcag cctctttcca gaaaccttat gcattgcaga 3361 tttgggttgt tctttgggag ctaacacttt cttggtggtc tcacagcttg ttaaaatagt 3421 agaaaaagaa cgaaaaaagc atggttttaa gtctccagag ttttattttc acttcaatga 3481 tcttcctggc aatgatttta atacactttt tcagtcactg ggggcatttc aagaagattt 3541 gagaaagcat ataggggaaa gctttggtcc atgttttttc agtggagtgc ctggttcatt 3601 ttatactaga cttttccctt ccaaaagttt acattttgtt tactcctcct acagtctcat 3661 gtggctatct caggtgccta atgggattga aaataacaag ggaaacattt acatggcaag 3721 aacaagccct ctaagtgtta ttaaagcata ctacaagcaa tatgaaatag atttttcaaa 3781 ttttctcaag taccgttcag aggaattgat gaaaggtgga aagatggtgt taacactcct 3841 aggtagagaa agtgaggatc ctactagcaa agaatgctgt tacatttggg agcttctagc 3901 catggccctc aataagttgg ttgaagaggg attgataaaa gaagagaaag tagatgcatt 3961 caatattcct caatacacac catcaccagc agaagtaaag tacatagttg agaaggaagg 4021 atcattcacc attaatcgct tggaaacatc aagagttcat tggaatgctt ctaataatga 4081 gaagaatggt ggttacaatg tgtcaaggtg catgagagct gtggctgagc ctttgcttgt 4141 cagccacttt gacaaggaat tgatggattt agtgttccac aagtacgaag agattgtttc 4201 tgattgcatg tccaaagaga atactgagtt tataaatgtc atcatctcct tgaccaaaat 4261 aaattaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 4321 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 4381 cgggtgggcc tttctgcgtt tata //