LOCUS       BBa_K1065105            1377 bp    DNA     linear       15-Jun-2024
DEFINITION  lac_promoter + RBS + SAM synthetase
ACCESSION   BBa_K1065105
VERSION     BBa_K1065105.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..215
                     /label=RBS
     misc_feature    216..222
                     /label=spacer
     CDS             223..1377
                     /label=SAM synthetase
     misc_feature    209..1377
                     /label=BBa_K1065104
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa ggaggaacta ctatggcaaa acaccttttt
      241 acgtccgagt ccgtctctga agggcatcct gacaaaattg ctgaccaaat ttctgatgcc
      301 gttttagacg cgatcctcga acaggatccg aaagcacgcg ttgcttgcga aacctacgta
      361 aaaaccggca tggttttagt tggcggcgaa atcaccacca gcgcctgggt agacatcgaa
      421 gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtgc attccgacat gggctttgac
      481 gctaactcct gtgcggttct gagcgctatc ggcaaacagt ctcctgacat caaccagggc
      541 gttgaccgtg ccgatccgct ggaacagggc gcgggtgacc agggtctgat gtttggctac
      601 gcaactaatg aaaccgacgt gctgatgcca gcacctatca cctatgcaca ccgtctggta
      661 cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa
      721 agccaggtga cttttcagta tgacgacggc aaaatcgttg gtatcgatgc tgtcgtgctt
      781 tccactcagc actctgaaga gatcgaccag aaatcgctgc aagaagcggt aatggaagag
      841 atcatcaagc caattctgcc cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac
      901 ccgaccggtc gtttcgttat cggtggccca atgggtgact gcggtctgac tggtcgtaaa
      961 attatcgttg atacctacgg cggcatggcg cgtcacggtg gcggtgcatt ctctggtaaa
     1021 gatccatcaa aagtggaccg ttccgcagcc tacgcagcac gttatgtcgc gaaaaacatc
     1081 gttgctgctg gcctggccga tcgttgtgaa attcaggttt cctacgcaat cggcgtggct
     1141 gaaccgacct ccatcatggt agaaactttc ggtactgaga aagtgccttc tgaacaactg
     1201 accctgctgg tacgtgagtt cttcgacctg cgcccatacg gtctgattca gatgctggat
     1261 ctgctgcacc cgatctacaa agaaaccgca gcatacggtc actttggtcg tgaacatttc
     1321 ccgtgggaaa aaaccgacaa agcgcagctg ctgcgcgatg ctgccggtct gaagtaa
//