##gff-version 3
##sequence-region BBa_K1065106 1 5789
BBa_K1065106	.	engineered_region	4413	5789	.	+	0	ID=BBa_K1065105;Name=BBa_K1065105
BBa_K1065106	.	engineered_region	4621	5789	.	+	0	ID=BBa_K1065104;Name=BBa_K1065104;Parent=BBa_K1065105
BBa_K1065106	.	ribosome_entry_site	4621	4627	.	+	0	ID=annotation2331779;Name=RBS;Parent=BBa_K1065104
BBa_K1065106	.	sequence_feature	4628	4634	.	+	0	ID=annotation2331780;Name=spacer;Parent=BBa_K1065104
BBa_K1065106	.	CDS	4635	5789	.	+	0	ID=annotation2331781;Name=SAM synthetase;Parent=BBa_K1065104
BBa_K1065106	.	promoter	4413	4612	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1065105
BBa_K1065106	.	engineered_region	4413	4612	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1065106	.	promoter	4549	4554	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1065106	.	non_covalent_binding_site	4578	4612	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1065106	.	non_covalent_binding_site	4501	4538	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1065106	.	CDS	4413	4500	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1065106	.	start_codon	4585	4585	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1065106	.	promoter	4573	4578	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1065106	.	engineered_region	1	4404	.	+	0	ID=BBa_K1065102;Name=BBa_K1065102
BBa_K1065106	.	engineered_region	1964	4404	.	+	0	ID=BBa_K1065101;Name=BBa_K1065101;Parent=BBa_K1065102
BBa_K1065106	.	promoter	1964	3166	.	+	0	ID=BBa_K731201;Name=BBa_K731201;Parent=BBa_K1065101
BBa_K1065106	.	CDS	1964	3043	.	+	0	ID=annotation2179313;Name=araC;Parent=BBa_K731201
BBa_K1065106	.	promoter	3006	3077	.	+	0	ID=annotation2179312;Name=PC;Parent=BBa_K731201
BBa_K1065106	.	promoter	3043	3166	.	+	0	ID=annotation2179311;Name=pBAD;Parent=BBa_K731201
BBa_K1065106	.	engineered_region	3175	4404	.	+	0	ID=BBa_K1065100;Name=BBa_K1065100;Parent=BBa_K1065101
BBa_K1065106	.	engineered_region	3175	4404	.	+	0	ID=BBa_J45119;Name=BBa_J45119;Parent=BBa_K1065100
BBa_K1065106	.	CDS	3194	4267	.	+	0	ID=BBa_J45004;Name=BBa_J45004;Parent=BBa_J45119
BBa_K1065106	.	stop_codon	4265	4267	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_K1065106	.	start_codon	3194	3196	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_K1065106	.	CDS	3194	4267	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
BBa_K1065106	.	terminator	4364	4404	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45119
BBa_K1065106	.	polyA_site	4391	4404	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1065106	.	stop_codon	4397	4397	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1065106	.	engineered_region	4364	4404	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1065106	.	stem_loop	4371	4390	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1065106	.	terminator	4276	4355	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45119
BBa_K1065106	.	stem_loop	4287	4330	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1065106	.	engineered_region	4276	4355	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1065106	.	ribosome_entry_site	3175	3187	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45119
BBa_K1065106	.	ribosome_entry_site	3175	3187	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1065106	.	engineered_region	3175	3187	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1065106	.	ribosome_entry_site	3181	3184	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1065106	.	engineered_region	1	1955	.	+	0	ID=BBa_J45320;Name=BBa_J45320;Parent=BBa_K1065102
BBa_K1065106	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J45320
BBa_K1065106	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1065106	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1065106	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1065106	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1065106	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1065106	.	terminator	1827	1906	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45320
BBa_K1065106	.	stem_loop	1838	1881	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1065106	.	engineered_region	1827	1906	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1065106	.	CDS	83	1818	.	+	0	ID=BBa_J45017;Name=BBa_J45017;Parent=BBa_J45320
BBa_K1065106	.	start_codon	385	387	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_K1065106	.	CDS	385	1818	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_K1065106	.	start_codon	83	85	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_K1065106	.	stop_codon	386	388	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_K1065106	.	CDS	83	388	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_K1065106	.	point_mutation	522	522	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_K1065106	.	stop_codon	1813	1818	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_K1065106	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45320
BBa_K1065106	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1065106	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1065106	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1065106	.	terminator	1915	1955	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45320
BBa_K1065106	.	polyA_site	1942	1955	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1065106	.	stop_codon	1948	1948	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1065106	.	engineered_region	1915	1955	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1065106	.	stem_loop	1922	1941	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K1065106
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtcc
aggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccga
gcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactg
ttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggct
ggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgt
ccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaa
cgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcga
acagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgc
ctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgc
tcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgc
cctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgacc
gctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtcc
gccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcat
cgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctcc
ccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatg
cccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggc
gatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtc
cagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccga
cccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggta
cgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccg
ggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgcctta
agctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaata
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaactt
gacggctacatcattcactttttcttcacaaccggcacgaaactcgctcgggctggccccggtgcattttttaaatact
cgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagca
gcttcgcctgactaatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacag
acgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtac
tgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaatt
gctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtc
gctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattca
tgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaa
tctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgacc
gcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctca
atcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatac
ttttcatactcccaccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactg
gctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaa
acgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgcca
tagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtt
tttttgtactagagtcacacaggaaagtactagatggaagttgttgaagttcttcacatgaatggaggaaatggagaca
gtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattga
tctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtg
gtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttca
atgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatatagg
ggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttacat
tttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttaca
tggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagta
ccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaa
gaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaag
tagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcac
cattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaagg
tgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtacg
aagagattgtttctgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaaaataaatta
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaac
cgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcg
caacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgt
ggaattgtgagcggataacaatttcacacatactagagaaggaggaactactatggcaaaacacctttttacgtccgag
tccgtctctgaagggcatcctgacaaaattgctgaccaaatttctgatgccgttttagacgcgatcctcgaacaggatc
cgaaagcacgcgttgcttgcgaaacctacgtaaaaaccggcatggttttagttggcggcgaaatcaccaccagcgcctg
ggtagacatcgaagagatcacccgtaacaccgttcgcgaaattggctatgtgcattccgacatgggctttgacgctaac
tcctgtgcggttctgagcgctatcggcaaacagtctcctgacatcaaccagggcgttgaccgtgccgatccgctggaac
agggcgcgggtgaccagggtctgatgtttggctacgcaactaatgaaaccgacgtgctgatgccagcacctatcaccta
tgcacaccgtctggtacagcgtcaggctgaagtgcgtaaaaacggcactctgccgtggctgcgcccggacgcgaaaagc
caggtgacttttcagtatgacgacggcaaaatcgttggtatcgatgctgtcgtgctttccactcagcactctgaagaga
tcgaccagaaatcgctgcaagaagcggtaatggaagagatcatcaagccaattctgcccgctgaatggctgacttctgc
caccaaattcttcatcaacccgaccggtcgtttcgttatcggtggcccaatgggtgactgcggtctgactggtcgtaaa
attatcgttgatacctacggcggcatggcgcgtcacggtggcggtgcattctctggtaaagatccatcaaaagtggacc
gttccgcagcctacgcagcacgttatgtcgcgaaaaacatcgttgctgctggcctggccgatcgttgtgaaattcaggt
ttcctacgcaatcggcgtggctgaaccgacctccatcatggtagaaactttcggtactgagaaagtgccttctgaacaa
ctgaccctgctggtacgtgagttcttcgacctgcgcccatacggtctgattcagatgctggatctgctgcacccgatct
acaaagaaaccgcagcatacggtcactttggtcgtgaacatttcccgtgggaaaaaaccgacaaagcgcagctgctgcg
cgatgctgccggtctgaagtaa