LOCUS BBa_K1065203 9512 bp DNA linear 25-Nov-2024 DEFINITION Efe+Bba_B0015 in pSpac (BBa_K823026) ACCESSION BBa_K1065203 VERSION BBa_K1065203.1 FEATURES Location/Qualifiers RBS 1..6 /label=RBS misc_feature 1..1078 /label=BBa_K1065000 misc_feature 7..13 /label=Spacer CDS 14..1078 /label=CDS - Ethylene Forming Enzyme misc_feature 14..16 /label=Start misc_feature 17..22 /label=NgoMIV misc_feature 1070..1075 /label=AgeI misc_feature 1076..1078 /label=Stop misc_feature 1..1078 /label=BBa_K1065000 stem_loop 1098..1141 /label=stem_loop misc_feature 1087..1166 /label=BBa_B0010 terminator 1087..1166 /label=BBa_B0010 stem_loop 1182..1201 /label=T7 TE misc_feature 1208..1208 /label=stop polyA_site 1202..1215 /label=polya misc_feature 1175..1215 /label=BBa_B0012 terminator 1175..1215 /label=BBa_B0012 terminator 1087..1215 /label=BBa_B0015 misc_feature 1..1215 /label=BBa_K1065002 CDS 1494..2576 /label=lacI CDS 3832..4692 /label=bla misc_feature 5316..6320 /label=repU CDS 6480..7250 /label=kan CDS 7473..7871 /label=ble CDS 8129..9094 /label=lacY promoter 9416..9481 /label=Pspac misc_feature 1224..9512 /label=BBa_K823026 ORIGIN 1 aggaggaact actatggccg gcaccaacct gcaaacattt gaactgccga cggaagtaac 61 tggctgcgct gcggacattt cgctgggacg tgccctgatc caagcatggc agaaagatgg 121 catttttcaa atcaaaaccg attcggaaca ggaccgtaaa acacaagaag ccatggcggc 181 atctaaacag ttctgcaaag aaccgctgac gtttaaaagc tcatgtgtca gcgatctgac 241 ttattcagga tacgtggcgt ctggcgaaga agttacggca ggcaaaccgg actttccgga 301 aatcttcact gtgtgcaaag atctgtcagt cggtgaccag agagtaaaag ctggttggcc 361 gtgtcatggc ccggtgccgt ggccgaacaa cacctaccaa aaaagcatga aaacattcat 421 ggaagaactg ggcctggccg gtgaacgcct gctgaaactg acggctctgg gtttcgaact 481 gccgattaat acgtttactg atctgacccg cgacggatgg catcacatgc gtgtgctgag 541 atttccgccg cagaccagta cactgtcgcg tggtattgga gcgcataccg attatggact 601 gctggtcatc gccgctcaag atgacgtagg cggtctgtac attcgtccgc cggtcgaagg 661 tgaaaaacgc aatcgtaact ggctgccggg cgaatcttcc gcaggtatgt tcgaacacga 721 tgaaccgtgg acctttgtaa cgccgactcc gggagtgtgg acagttttcc cgggcgacat 781 cctgcaattt atgacgggag gccaactgct gtccactccg cataaagtta aactgaatac 841 aagagaacgc tttgcgtgcg catatttcca cgaaccgaac tttgaagcct ccgcttaccc 901 gctgtttgaa ccgagtgcaa atgaacgcat tcattatggc gaacacttta cgaacatgtt 961 catgcgctgt tacccggatc gtattaccac acaaagaatc aacaaagaaa atagactggc 1021 acacctggaa gacctgaaaa aatactcgga cacaagagca actggttcga ccggttaata 1081 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1141 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1201 ctttctgcgt ttatatacta gagtactagt agcggccgct gcaggcatgc aagctaattc 1261 cggtggaaac gaggtcatca tttccttccg aaaaaacggt tgcatttaaa tcttacatat 1321 gtaatacttt caaagactac atttgtaaga tttgatgttt gagtcggctg aaagatcgta 1381 cgtaccaatt attgtttcgt gattgttcaa gccataacac tgtagggata gtggaaagag 1441 tgcttcatct ggttacgatc aatcaaatat tcaaacggag ggagacgatt ttgatgaaac 1501 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 1561 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 1621 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 1681 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 1741 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 1801 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 1861 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 1921 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 1981 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 2041 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 2101 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 2161 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 2221 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 2281 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 2341 tcccgccgtc aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 2401 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 2461 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 2521 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 2581 aacgcaatta atgtgagtta ggatcctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 2641 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 2701 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 2761 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt 2821 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 2881 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 2941 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 3001 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 3061 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 3121 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 3181 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 3241 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 3301 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 3361 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 3421 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 3481 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 3541 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 3601 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 3661 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 3721 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 3781 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 3841 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 3901 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 3961 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 4021 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 4081 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 4141 cattgctgcc ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 4201 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 4261 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 4321 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 4381 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 4441 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 4501 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 4561 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 4621 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 4681 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 4741 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 4801 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 4861 taaaaatagg cgtatcacga ggccctttcg tcttcaagag ttccttaagg aacgtacaga 4921 cggcttaaaa gcctttaaaa acgtttttaa ggggtttgta gacaaggtaa aggataaaac 4981 agcacaattc caagaaaaac acgatttaga acctaaaaag aacgaatttg aactaactca 5041 taaccgagag gtaaaaaaag aacgaagtcg agatcaggga atgagtttat aaaataaaaa 5101 aagcacctga aaaggtgtct ttttttgatg gttttgaact tgttctttct tatcttgata 5161 catatagaaa taacgtcatt tttattttag ttgctgaaag gtgcgttgaa gtgttggtat 5221 gtatgtgttt taaagtattg aaaaccctta aaattggttg cacagaaaaa ccccatctgt 5281 taaagttata agtgactaaa caaataacta aatagatggg ggtttctttt aatattatgt 5341 gtcctaatag tagcatttat tcagatgaaa aatcaagggt tttagtggac aagacaaaaa 5401 gtggaaaagt gagaccatgg agagaaaaga aaatcgctaa tgttgattac tttgaacttc 5461 tgcatattct tgaatttaaa aaggctgaaa gagtaaaaga ttgtgctgaa atattagagt 5521 ataaacaaaa tcgtgaaaca ggcgaaagaa agttgtatcg agtgtggttt tgtaaatcca 5581 ggctttgtcc aatgtgcaac tggaggagag caatgaaaca tggcattcag tcacaaaagg 5641 ttgttgctga agttattaaa caaaagccaa cagttcgttg gttgtttctc acattaacag 5701 ttaaaaatgt ttatgatggc gaagaattaa ataagagttt gtcagatatg gctcaaggat 5761 ttcgccgaat gatgcaatat aaaaaaatta ataaaaatct tgttggtttt atgcgtgcaa 5821 cggaagtgac aataaataat aaagataatt cttataatca gcacatgcat gtattggtat 5881 gtgtggaacc aacttatttt aagaatacag aaaactacgt gaatcaaaaa caatggattc 5941 aattttggaa aaaggcaatg aaattagact atgatccaaa tgtaaaagtt caaatgattc 6001 gaccgaaaaa taaatataaa tcggatatac aatcggcaat tgacgaaact gcaaaatatc 6061 ctgtaaagga tacggatttt atgaccgatg atgaagaaaa gaatttgaaa cgtttgtctg 6121 atttggagga aggtttacac cgtaaaaggt taatctccta tggtggtttg ttaaaagaaa 6181 tacataaaaa attaaacctt gatgacacag aagaaggcga tttgattcat acagatgatg 6241 acgaaaaagc cgatgaagat ggattttcta ttattgcaat gtggaattgg gaacggaaaa 6301 attattttat taaagagtag ttcaacaaac gggccagttt gttgaagatt agatgctata 6361 attgttatta aaaggattga aggatgctta ggaagacgag ttattaatag ctgaataaga 6421 acggtgctct ccaaatattc ttatttagaa aagcaaatct aaaattatct gaaaagggaa 6481 tgagaatagt gaatggacca ataataatga ctagagaaga aagaatgaag attgttcatg 6541 aaattaagga acgaatattg gataaatatg gggatgatgt taaggctatt ggtgtttatg 6601 gctctcttgg tcgtcagact gatgggccct attcggatat tgagatgatg tgtgtcatgt 6661 caacagagga agcagagttc agccatgaat ggacaaccgg tgagtggaag gtggaagtga 6721 attttgatag cgaagagatt ctactagatt atgcatctca ggtggaatca gattggccgc 6781 ttacacatgg tcaatttttc tctattttgc cgatttatga ttcaggtgga tacttagaga 6841 aagtgtatca aactgctaaa tcggtagaag cccaaacgtt ccacgatgcg atttgtgccc 6901 ttatcgtaga agagctgttt gaatatgcag gcaaatggcg taatattcgt gtgcaaggac 6961 cgacaacatt tctaccatcc ttgactgtac aggtagcaat ggcaggtgcc atgttgattg 7021 gtctgcatca tcgcatctgt tatacgacga gcgcttcggt cttaactgaa gcagttaagc 7081 aatcagatct tccttcaggt tatgaccatc tgtgccagtt cgtaatgtct ggtcaacttt 7141 ccgactctga gaaacttctg gaatcgctag agaatttctg gaatgggatt caggagtgga 7201 cagaacgaca cggatatata gtggatgtgt caaaacgcat accattttga acgatgacct 7261 ctaataattg ttaatcatgt tggttacgta tttattaact tctcctagta ttagtaatta 7321 tcatggctgt catggcgcat taacggaata aagggtgtgc ttaaatcggg ccattttgcg 7381 taataagaaa aaggattaat tatgagcgaa ttgaattaat aataaggtaa tagatttaca 7441 ttagaaaatg aaaggggatt ttatgcgtga gaatgttaca gtctatcccg gcattgccag 7501 tcggggatat taaaaagagt ataggttttt attgcgataa actaggtttc actttggttc 7561 accatgaaga tggattcgca gttctaatgt gtaatgaggt tcggattcat ctatgggagg 7621 caagtgatga aggctggcgc tctcgtagta atgattcacc ggtttgtaca ggtgcggagt 7681 cgtttattgc tggtactgct agttgccgca ttgaagtaga gggaattgat gaattatatc 7741 aacatattaa gcctttgggc attttgcacc ccaatacatc attaaaagat cagtggtggg 7801 atgaacgaga ctttgcagta attgatcccg acaacaattt gattagcttt tttcaacaaa 7861 taaaaagcta aaatctatta ttaatctgtt cagcaatcgg gcgcgattgc tgaataaaag 7921 atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgtccgt tacactagaa 7981 aaccgaaaga caataaaaat tttattcttg ctgagtctgg ctttcggtaa gctagacaaa 8041 acggacaaaa taaaaattgg caagggttta aaggtggaga ttttttgagt gatcttctca 8101 aaaaatacta cctgtccctt gctgattttt aaacgagcac gagagcaaaa cccccctttg 8161 ctgaggtggc gaacgatgag ccaataatac gtacagacat aatagtgcca gccagcagca 8221 gggcgttttt cccaccgatg cgattaatga tcagtggcgc aaagaacata atcgaggcgt 8281 taagtaattc gcccattgtc gttacgtagc caaatacccg cgtaccctgt tcaccggtag 8341 caaagaacga agtaaagaaa ttagcaaact gttggtcaaa aacatcgtag gtgcaggaaa 8401 cgccaataac atacagtgac aaaaaccaca gttttggctg tctgaacagt tccagtgcca 8461 gcttaaggct aaatgccgaa tggttggcac ctaccgcatt ggcaaccgtg gcagaagagg 8521 gcgcatccgt tttggcgaaa aagagtaaaa cggcgaggat gagtgcacag ccagagccca 8581 gccagaaaac aaactgatta ttgatggtga acatgatgcc gacaatcgag gcacacagcg 8641 cccagccaac acagccaaac atccgcgcgc gaccaaattc gaaattactg cgacggctga 8701 ctttctcaat aaatgcctct actgctggcg caccggcgtt aaaacaaaag cctagataaa 8761 taccaccaac aatcgatcct actaaaatgt tgtattgtaa cagtggcccg aagataaaaa 8821 taaagaacgg cgcaaacatc actaacatgc cggtaataat ccacagcagg tatttgcgca 8881 gcccgagttt gtcagaaagc agaccaaaca gcggttggaa taatagcgag aacagagaaa 8941 tagcggcaaa aataataccc gtatcacttt tgctgatatg gttgatgtca tgtagccaaa 9001 tcgggaaaaa cgggaagtag gctcccatga taaaaaagta aaagaaaaag aataaaccga 9061 acatccaaaa gtttgtgttt tttaaatagt acataatgga tttccttacg cgaaatacgg 9121 gcagacatgg cctgcccggt tattattatt tttgacacca gaccaactgg taatggtagc 9181 gaccggcgct cagctggagt tctacacagc ccagtccaga ctattcggca ctgaaattat 9241 gggtgaagtg gtcaagacct cactaggcac cttaaaaata gcgcaccctg aagaagattt 9301 atttgaggta gcccttgcct acctagcttc caagaaagat atcctaacag cacaagagcg 9361 gaaagatgtt ttgttctaca tccagaacaa cctctgctaa aattcctgaa aaattttgca 9421 aaaagttgtt gactttatct acaaggtgtg gcataatgtg tggaattgtg agcggataac 9481 aattaagctt gaattcgcgg ccgcttctag ag //