spac+efe
type: ComponentDefinition
persistent identity: BBa K1065203
display id: BBa_K1065203
version: 1
was generated by: iGEM to SBOL conversion
description: Efe+Bba_B0015 in pSpac (BBa_K823026)
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:08:59Z
mutable provenance: This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too.
owned by: james
owned by: myers
mutable description: 2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pSpac vector that has benn sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.
discontinued: false
dominant: false
experience: experience/None
group u list: _1373_
m user id: 0
owner id: 15998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The coding sequence was taken from Pseudomonas syringae pv. phaseolicola PK2. We firstly optimized the codons for both B. subtilis and E. coli and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly.
star: false
creator: Viola Valentini
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1065002
component: BBa_K823026
sequence annotation: BBa_K1065002
sequence annotation: BBa_K823026
sequence: https://synbiohub.org/public/igem/BBa_K1065203_sequence/1

BBa_K1065002
type: Component
persistent identity: component2334235
display id: component2334235
version: 1
top level: spac+efe
definition: EFE + T
access: public

BBa_K823026
type: Component
persistent identity: component2334243
display id: component2334243
version: 1
top level: spac+efe
definition: pSBBs0K
access: public

BBa_K1065002
type: SequenceAnnotation
persistent identity: annotation2334235
display id: annotation2334235
version: 1
top level: spac+efe
location: https://synbiohub.org/public/igem/BBa_K1065203/annotation2334235/range2334235/1
component: BBa_K1065002

https://synbiohub.org/public/igem/BBa_K1065203/annotation2334235/range2334235/1
type: Range
persistent identity: range2334235
display id: range2334235
version: 1
top level: spac+efe
direction: direction/reverse
start: 1
end: 1215
orientation: inline

BBa_K823026
type: SequenceAnnotation
persistent identity: annotation2334243
display id: annotation2334243
version: 1
top level: spac+efe
location: https://synbiohub.org/public/igem/BBa_K1065203/annotation2334243/range2334243/1
component: BBa_K823026

https://synbiohub.org/public/igem/BBa_K1065203/annotation2334243/range2334243/1
type: Range
persistent identity: range2334243
display id: range2334243
version: 1
top level: spac+efe
direction: direction/reverse
start: 1224
end: 9512
orientation: inline

pSBBs0K
type: ComponentDefinition
persistent identity: BBa K823026
display id: BBa_K823026
version: 1
was generated by: iGEM to SBOL conversion
description: pSBBs0K-Pspac (replicative Bacillus subtilis expression vector; IPTG inducible
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
mutable provenance: This part is an igem compatible version of the Bacillus expression vector pDG148
owned by: james
owned by: myers
mutable description: This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has a ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis. The multiple cloning site is downstream of a Pspac promoter, that is inducible with IPTG. (Isopropyl-β-D-thiogalactopyranosid). This BioBrick is part of the LMU 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli. It can be used as an inducible expression vector in B. subtilis.
star: false
creator: Jara Radeck
type: DnaRegion
role: partType/Plasmid Backbone
role: SO:0000755
sequence annotation: lacI
sequence annotation: bla
sequence annotation: repU
sequence annotation: kan
sequence annotation: ble
sequence annotation: lacY
sequence annotation: Pspac
sequence: https://synbiohub.org/public/igem/BBa_K823026_sequence/1

lacI
type: SequenceAnnotation
persistent identity: annotation2180256
display id: annotation2180256
version: 1
top level: pSBBs0K
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180256/range2180256/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K823026/annotation2180256/range2180256/1
type: Range
persistent identity: range2180256
display id: range2180256
version: 1
top level: pSBBs0K
direction: direction/forward
start: 271
end: 1353
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180257
display id: annotation2180257
version: 1
top level: pSBBs0K
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180257/range2180257/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K823026/annotation2180257/range2180257/1
type: Range
persistent identity: range2180257
display id: range2180257
version: 1
top level: pSBBs0K
direction: direction/reverse
start: 2609
end: 3469
orientation: inline

repU
type: SequenceAnnotation
persistent identity: annotation2180258
display id: annotation2180258
version: 1
top level: pSBBs0K
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180258/range2180258/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K823026/annotation2180258/range2180258/1
type: Range
persistent identity: range2180258
display id: range2180258
version: 1
top level: pSBBs0K
direction: direction/forward
start: 4093
end: 5097
orientation: inline

kan
type: SequenceAnnotation
persistent identity: annotation2180259
display id: annotation2180259
version: 1
top level: pSBBs0K
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180259/range2180259/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K823026/annotation2180259/range2180259/1
type: Range
persistent identity: range2180259
display id: range2180259
version: 1
top level: pSBBs0K
direction: direction/forward
start: 5257
end: 6027
orientation: inline

ble
type: SequenceAnnotation
persistent identity: annotation2180260
display id: annotation2180260
version: 1
top level: pSBBs0K
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180260/range2180260/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K823026/annotation2180260/range2180260/1
type: Range
persistent identity: range2180260
display id: range2180260
version: 1
top level: pSBBs0K
direction: direction/forward
start: 6250
end: 6648
orientation: inline

lacY
type: SequenceAnnotation
persistent identity: annotation2180261
display id: annotation2180261
version: 1
top level: pSBBs0K
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180261/range2180261/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K823026/annotation2180261/range2180261/1
type: Range
persistent identity: range2180261
display id: range2180261
version: 1
top level: pSBBs0K
direction: direction/reverse
start: 6906
end: 7871
orientation: inline

Pspac
type: SequenceAnnotation
persistent identity: annotation2180262
display id: annotation2180262
version: 1
top level: pSBBs0K
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180262/range2180262/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K823026/annotation2180262/range2180262/1
type: Range
persistent identity: range2180262
display id: range2180262
version: 1
top level: pSBBs0K
direction: direction/forward
start: 8193
end: 8258
orientation: inline

EFE + T
type: ComponentDefinition
persistent identity: BBa K1065002
display id: BBa_K1065002
version: 1
was generated by: iGEM to SBOL conversion
description: EFE + Terminators
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:08:59Z
mutable provenance: c
owned by: james
owned by: myers
mutable description: BMST1 in psB1C3
discontinued: false
dominant: false
experience: experience/Works
group u list: _1373_
m user id: 0
owner id: 15968
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: c
star: false
creator: Thomas Perli
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K1065000
sequence annotation: BBa_K1065000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1065002_sequence/1

BBa_B0015
type: Component
persistent identity: component2331758
display id: component2331758
version: 1
top level: EFE + T
definition: BBa_B0015
access: public

BBa_K1065000
type: Component
persistent identity: component2331744
display id: component2331744
version: 1
top level: EFE + T
definition: EFE
access: public

BBa_K1065000
type: SequenceAnnotation
persistent identity: annotation2331744
display id: annotation2331744
version: 1
top level: EFE + T
location: https://synbiohub.org/public/igem/BBa_K1065002/annotation2331744/range2331744/1
component: BBa_K1065000

https://synbiohub.org/public/igem/BBa_K1065002/annotation2331744/range2331744/1
type: Range
persistent identity: range2331744
display id: range2331744
version: 1
top level: EFE + T
direction: direction/reverse
start: 1
end: 1078
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2331758
display id: annotation2331758
version: 1
top level: EFE + T
location: https://synbiohub.org/public/igem/BBa_K1065002/annotation2331758/range2331758/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1065002/annotation2331758/range2331758/1
type: Range
persistent identity: range2331758
display id: range2331758
version: 1
top level: EFE + T
direction: direction/reverse
start: 1087
end: 1215
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

EFE
type: ComponentDefinition
persistent identity: BBa K1065000
display id: BBa_K1065000
version: 1
was generated by: iGEM to SBOL conversion
description: 2-oxoglutarate oxygenase/decarboxylase (EFE)
created: 2013-06-19T11:00:00Z
modified: 2015-05-08T01:08:59Z
mutable provenance: xxxx
owned by: james
owned by: myers
mutable description: xxx
discontinued: false
dominant: false
experience: experience/Works
group u list: _1373_
m user id: 0
owner id: 15969
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: xxx
star: false
creator: Thomas Perli
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: BBa_K1065000
sequence annotation: Spacer
sequence annotation: CDS - Ethylene Forming Enzyme
sequence annotation: Start
sequence annotation: NgoMIV
sequence annotation: AgeI
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K1065000_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2331672
display id: annotation2331672
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331672/range2331672/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331672/range2331672/1
type: Range
persistent identity: range2331672
display id: range2331672
version: 1
top level: EFE
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1065000
type: SequenceAnnotation
persistent identity: annotation2331673
display id: annotation2331673
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331673/range2331673/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331673/range2331673/1
type: Range
persistent identity: range2331673
display id: range2331673
version: 1
top level: EFE
direction: direction/reverse
start: 1
end: 1078
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2331674
display id: annotation2331674
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331674/range2331674/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331674/range2331674/1
type: Range
persistent identity: range2331674
display id: range2331674
version: 1
top level: EFE
direction: direction/forward
start: 7
end: 13
orientation: inline

CDS - Ethylene Forming Enzyme
type: SequenceAnnotation
persistent identity: annotation2331675
display id: annotation2331675
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331675/range2331675/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331675/range2331675/1
type: Range
persistent identity: range2331675
display id: range2331675
version: 1
top level: EFE
direction: direction/forward
start: 14
end: 1078
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2331676
display id: annotation2331676
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331676/range2331676/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331676/range2331676/1
type: Range
persistent identity: range2331676
display id: range2331676
version: 1
top level: EFE
direction: direction/forward
start: 14
end: 16
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2331677
display id: annotation2331677
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331677/range2331677/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331677/range2331677/1
type: Range
persistent identity: range2331677
display id: range2331677
version: 1
top level: EFE
direction: direction/forward
start: 17
end: 22
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2331678
display id: annotation2331678
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331678/range2331678/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331678/range2331678/1
type: Range
persistent identity: range2331678
display id: range2331678
version: 1
top level: EFE
direction: direction/forward
start: 1070
end: 1075
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2331679
display id: annotation2331679
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331679/range2331679/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331679/range2331679/1
type: Range
persistent identity: range2331679
display id: range2331679
version: 1
top level: EFE
direction: direction/forward
start: 1076
end: 1078
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1065203_sequence/1
type: Sequence
persistent identity: BBa K1065203 sequence
display id: BBa_K1065203_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtactagtagcggccgctgcaggcatgcaagctaattccggtggaaacgaggtcatcatttccttccgaaaaaacggttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttgatgtttgagtcggctgaaagatcgtacgtaccaattattgtttcgtgattgttcaagccataacactgtagggatagtggaaagagtgcttcatctggttacgatcaatcaaatattcaaacggagggagacgattttgatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823026_sequence/1
type: Sequence
persistent identity: BBa K823026 sequence
display id: BBa_K823026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcaggcatgcaagctaattccggtggaaacgaggtcatcatttccttccgaaaaaacggttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttgatgtttgagtcggctgaaagatcgtacgtaccaattattgtttcgtgattgttcaagccataacactgtagggatagtggaaagagtgcttcatctggttacgatcaatcaaatattcaaacggagggagacgattttgatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttaggatcctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1065002_sequence/1
type: Sequence
persistent identity: BBa K1065002 sequence
display id: BBa_K1065002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1065000_sequence/1
type: Sequence
persistent identity: BBa K1065000 sequence
display id: BBa_K1065000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z