LOCUS BBa_K1065204 6017 bp DNA linear 12-Nov-2024 DEFINITION Efe+Bba_B0015 in BBa_K823024 (pXyl) ACCESSION BBa_K1065204 VERSION BBa_K1065204.1 FEATURES Location/Qualifiers RBS 1..6 /label=RBS misc_feature 1..1078 /label=BBa_K1065000 misc_feature 7..13 /label=Spacer CDS 14..1078 /label=CDS - Ethylene Forming Enzyme misc_feature 14..16 /label=Start misc_feature 17..22 /label=NgoMIV misc_feature 1070..1075 /label=AgeI misc_feature 1076..1078 /label=Stop misc_feature 1..1078 /label=BBa_K1065000 stem_loop 1098..1141 /label=stem_loop misc_feature 1087..1166 /label=BBa_B0010 terminator 1087..1166 /label=BBa_B0010 stem_loop 1182..1201 /label=T7 TE misc_feature 1208..1208 /label=stop polyA_site 1202..1215 /label=polya misc_feature 1175..1215 /label=BBa_B0012 terminator 1175..1215 /label=BBa_B0012 terminator 1087..1215 /label=BBa_B0015 misc_feature 1..1215 /label=BBa_K1065002 CDS 1434..2108 /label=spc misc_feature 2251..2679 /label='thrC misc_feature 2676..3086 /label=thrB CDS 3939..4799 /label=bla misc_feature 4954..5256 /label=hom misc_feature 5256..5795 /label=thrC' promoter 5762..5986 /label=Pxly region misc_feature 1224..6017 /label=BBa_K823024 ORIGIN 1 aggaggaact actatggccg gcaccaacct gcaaacattt gaactgccga cggaagtaac 61 tggctgcgct gcggacattt cgctgggacg tgccctgatc caagcatggc agaaagatgg 121 catttttcaa atcaaaaccg attcggaaca ggaccgtaaa acacaagaag ccatggcggc 181 atctaaacag ttctgcaaag aaccgctgac gtttaaaagc tcatgtgtca gcgatctgac 241 ttattcagga tacgtggcgt ctggcgaaga agttacggca ggcaaaccgg actttccgga 301 aatcttcact gtgtgcaaag atctgtcagt cggtgaccag agagtaaaag ctggttggcc 361 gtgtcatggc ccggtgccgt ggccgaacaa cacctaccaa aaaagcatga aaacattcat 421 ggaagaactg ggcctggccg gtgaacgcct gctgaaactg acggctctgg gtttcgaact 481 gccgattaat acgtttactg atctgacccg cgacggatgg catcacatgc gtgtgctgag 541 atttccgccg cagaccagta cactgtcgcg tggtattgga gcgcataccg attatggact 601 gctggtcatc gccgctcaag atgacgtagg cggtctgtac attcgtccgc cggtcgaagg 661 tgaaaaacgc aatcgtaact ggctgccggg cgaatcttcc gcaggtatgt tcgaacacga 721 tgaaccgtgg acctttgtaa cgccgactcc gggagtgtgg acagttttcc cgggcgacat 781 cctgcaattt atgacgggag gccaactgct gtccactccg cataaagtta aactgaatac 841 aagagaacgc tttgcgtgcg catatttcca cgaaccgaac tttgaagcct ccgcttaccc 901 gctgtttgaa ccgagtgcaa atgaacgcat tcattatggc gaacacttta cgaacatgtt 961 catgcgctgt tacccggatc gtattaccac acaaagaatc aacaaagaaa atagactggc 1021 acacctggaa gacctgaaaa aatactcgga cacaagagca actggttcga ccggttaata 1081 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1141 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1201 ctttctgcgt ttatatacta gagtactagt agcggccgct gcaggataaa aaatttagaa 1261 gccaatgaaa tctataaata aactaaatta agtttattta attaacaact atggatataa 1321 aataggtact aatcaaaata gtgaggagga tatatttgaa tacatacgaa caaattaata 1381 aagtgaaaaa aatacttcgg aaacatttaa aaaataacct tattggtact tacatgtttg 1441 gatcaggagt tgagagtgga ctaaaaccaa atagtgatct tgacttttta gtcgtcgtat 1501 ctgaaccatt gacagatcaa agtaaagaaa tacttataca aaaaattaga cctatttcaa 1561 aaaaaatagg agataaaagc aacttacgat atattgaatt aacaattatt attcagcaag 1621 aaatggtacc gtggaatcat cctcccaaac aagaatttat ttatggagaa tggttacaag 1681 agctttatga acaaggatac attcctcaga aggaattaaa ttcagattta accataatgc 1741 tttaccaagc aaaacgaaaa aataaaagaa tatacggaaa ttatgactta gaggaattac 1801 tacctgatat tccattttct gatgtgagaa gagccattat ggattcgtca gaggaattaa 1861 tagataatta tcaggatgat gaaaccaact ctatattaac tttatgccgt atgattttaa 1921 ctatggacac gggtaaaatc ataccaaaag atattgcggg aaatgcagtg gctgaatctt 1981 ctccattaga acatagggag agaattttgt tagcagttcg tagttatctt ggagagaata 2041 ttgaatggac taatgaaaat gtaaatttaa ctataaacta tttaaataac agattaaaaa 2101 aattataaaa aaattgaaaa aatggtggaa acactttttt caattttttt gttttattat 2161 ttaatatttg ggaaatattc attctaattg gtaatcagat tttagaaaac aataaaccct 2221 tgcatagggg gatctcgagg ccttccgaaa atgcgcggct ttgaagctga gggtgcggcg 2281 gcaatcgtgc gcaatgaagt gattgaaaat ccggaaacaa tagcgacagc cattcgtatc 2341 ggaaacccgg caagctggga caaagctgta aaggcagccg aggaatccaa tgggaaaatt 2401 gacgaagtca ctgatgatga aatccttcac gcatatcagc tgatcgcccg tgtagaaggc 2461 gtgtttgcag aaccaggttc ttgcgcgtct atcgcaggag tgctgaaaca ggtgaaatcc 2521 ggagaaattc cgaaaggcag caaggtcgta gctgtgttaa caggaaacgg actgaaagat 2581 ccgaacacag cggtcgacat ttcagaaatc aagcctgtca cattgccgac tgatgaagac 2641 agcatccttg aatatgtaaa aggagcggcc cgtgtatgaa cgaagccgac atgctgttct 2701 ctgtcactgt tcccggaagc acagctaacc taggccccgg ctttgattca gtcggaatgg 2761 cgctcagcag atatttgaag ctgaccgtct ttgaaagcga caaatggtct tttgaggctg 2821 aaacagaaac agtcgccgga attaattcga gctcctttaa cttcctcacc agtagttgta 2881 tcggtaccat aagtagaagc agcaacccaa gtagctttac cagcatccgg ttcaaccagc 2941 atagtaagaa tcttactgga catcggcagt tcttcgaaca gtgcgccaac taccagctct 3001 ttctacagtt cattcagggc accggagaac ctgcgtgcaa tccatcttgt tcaatcatgc 3061 gaaacgatcc tcatcctgtc tcttgatcca tggattacgc gcgaccttca gcatcgccgg 3121 catgtccccc tggcggacgg gaagtatcca gctcgaggtc gggccgcgtt gctggcgttt 3181 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 3241 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 3301 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 3361 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 3421 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 3481 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 3541 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 3601 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 3661 ttcggaaaaa gagttgatag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 3721 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 3781 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 3841 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 3901 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 3961 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 4021 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 4081 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 4141 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 4201 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgccggc 4261 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 4321 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 4381 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 4441 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 4501 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg 4561 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 4621 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 4681 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 4741 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 4801 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 4861 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 4921 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 4981 atcacgaggc cctttcgtct tcaagaatta attcatgtaa aagatgaggt tggttcattc 5041 tcgaaaatta catctgtgtt ctcagagcgg ggcgtgacgt ttgaaaaaat ccttcagctg 5101 ccaattaaag gccatgatga gttagctgaa atcgtaattg tcacacatca tacatcagaa 5161 gctgatttca gtgatatcct gcaaaaccta aatgatttgg aagtcgttca agaagtcaaa 5221 agcacatatc gtgtagaagg gaacggttgg agctaatgtg gaaaggactt atccatcaat 5281 ataaagaatt tttacctgta acagatcaaa caccggcgct aactttacat gaaggaaaca 5341 cacctcttat tcacctgccg aagctgtctg agcagctcgg aattgagctt catgtcaaaa 5401 cggaaggcgt caatcctacg ggatcattta aagatcgcgg aatggttatg gctgtggcaa 5461 aggcaaaaga agaaggcaat gacacgatta tgtgcgcgtc aacaggtaac acttccgctg 5521 ctgcggcagc atatgcagcc cgtgctaaca tgaaatgcat tgtcatcatc ccgaacggaa 5581 aaattgcatt tggaaaactc gctcaagctg tcatgtacgg agccgagatt atcgcaattg 5641 acggaaactt tgacgatgcg cttaaaattg tccgttccat ctgtgagaaa tcaccgattg 5701 cccttgtcaa ctcagtcaac ccttaccgca ttgaaggcca aaaaactgct gccttcggat 5761 cagcgatatc cacttcatcc actccatttg tttaatcttt aaattaagta tcaacatagt 5821 acatagcgaa tcttcccttt attatatcta atgtgttcat aaaaaactaa aaaaaatatt 5881 gaaaatactg acgaggttat ataagatgaa aataagttag tttgtttaaa caacaaacta 5941 ataggtgatg tacttactat atgaaataaa atgcatctgg gatcccaagc ttatcgaatt 6001 cgcggccgct tctagag //