BBa_K1065204 1 xyl+efe Efe+Bba_B0015 in BBa_K823024 (pXyl) 2013-08-31T11:00:00Z 2015-05-08T01:08:59Z This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too. 2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pXyl vector that has been sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki. false false _1373_ 0 15998 9 Not in stock false ===Design Notes=== <html> The coding sequence was taken from <I>Pseudomonas syringae</I> pv. <I>phaseolicola</I> PK2. We firstly optimized the codons for both <I>B. subtilis</I> and <I>E. coli</I> and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly.<br /> <br /> <span style= "color:brown" title="Prefix"><B>GAATTCGCGGCCGCTTCTAGA</B></span>TA<span style="color:purple; font-weight:bold" title="RBS">AGGAGG</span>AACTACT<b title="Start">ATG</b><span style="color:green" title="NgoMIV"><B>GCCGGC</B></span>ACC...<br/> <br/> ...TCG<span style="color:red; font-weight:bold" title="AgeI">ACCGGT</span><b title="Stop">TAA</b><span style="color:blue; font-weight:bold" title="suffix">TACTAGTAGCGGCCGCTGCAG</span> </html> false Viola Valentini component2334267 1 BBa_K823024 component2334259 1 BBa_K1065002 annotation2334267 1 BBa_K823024 range2334267 1 1224 6017 annotation2334259 1 BBa_K1065002 range2334259 1 1 1215 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1065000 1 EFE <html>2-oxoglutarate oxygenase/decarboxylase (EFE)</html> 2013-06-19T11:00:00Z 2015-05-08T01:08:59Z xxxx xxx false false _1373_ 0 15969 9 Not in stock false xxx false Thomas Perli annotation2331673 1 BBa_K1065000 range2331673 1 1 1078 annotation2331676 1 Start range2331676 1 14 16 annotation2331677 1 NgoMIV range2331677 1 17 22 annotation2331679 1 Stop range2331679 1 1076 1078 annotation2331675 1 CDS - Ethylene Forming Enzyme range2331675 1 14 1078 annotation2331678 1 AgeI range2331678 1 1070 1075 annotation2331672 1 RBS range2331672 1 1 6 annotation2331674 1 Spacer range2331674 1 7 13 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K823024 1 pSBBs4S pSB<sub>Bs</sub>4S-P<i><sub>xyl</i></sub>: Integrative expression vector for <i>Bacillus subtilis</i 2012-08-20T11:00:00Z 2015-05-08T01:13:30Z This part is an igem compatible version of the Bacillus expression vector pXT This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks false false _1081_ 0 11555 9 It's complicated false We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli. false Jara Radeck annotation2180243 1 bla range2180243 1 2716 3576 annotation2180242 1 thrB range2180242 1 1453 1863 annotation2180244 1 hom range2180244 1 3731 4033 annotation2180245 1 thrC' range2180245 1 4033 4572 annotation2180241 1 'thrC range2180241 1 1028 1456 annotation2180240 1 spc range2180240 1 211 885 annotation2180246 1 Pxly region range2180246 1 4539 4763 BBa_K1065002 1 EFE + T EFE + Terminators 2013-06-20T11:00:00Z 2015-05-08T01:08:59Z c BMST1 in psB1C3 false false _1373_ 0 15968 9 In stock true c false Thomas Perli component2331744 1 BBa_K1065000 component2331758 1 BBa_B0015 annotation2331758 1 BBa_B0015 range2331758 1 1087 1215 annotation2331744 1 BBa_K1065000 range2331744 1 1 1078 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1065000_sequence 1 aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaa BBa_K1065002_sequence 1 aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K823024_sequence 1 tactagtagcggccgctgcaggataaaaaatttagaagccaatgaaatctataaataaactaaattaagtttatttaattaacaactatggatataaaataggtactaatcaaaatagtgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataaaaaaattgaaaaaatggtggaaacacttttttcaatttttttgttttattatttaatatttgggaaatattcattctaattggtaatcagattttagaaaacaataaacccttgcatagggggatctcgaggccttccgaaaatgcgcggctttgaagctgagggtgcggcggcaatcgtgcgcaatgaagtgattgaaaatccggaaacaatagcgacagccattcgtatcggaaacccggcaagctgggacaaagctgtaaaggcagccgaggaatccaatgggaaaattgacgaagtcactgatgatgaaatccttcacgcatatcagctgatcgcccgtgtagaaggcgtgtttgcagaaccaggttcttgcgcgtctatcgcaggagtgctgaaacaggtgaaatccggagaaattccgaaaggcagcaaggtcgtagctgtgttaacaggaaacggactgaaagatccgaacacagcggtcgacatttcagaaatcaagcctgtcacattgccgactgatgaagacagcatccttgaatatgtaaaaggagcggcccgtgtatgaacgaagccgacatgctgttctctgtcactgttcccggaagcacagctaacctaggccccggctttgattcagtcggaatggcgctcagcagatatttgaagctgaccgtctttgaaagcgacaaatggtcttttgaggctgaaacagaaacagtcgccggaattaattcgagctcctttaacttcctcaccagtagttgtatcggtaccataagtagaagcagcaacccaagtagctttaccagcatccggttcaaccagcatagtaagaatcttactggacatcggcagttcttcgaacagtgcgccaactaccagctctttctacagttcattcagggcaccggagaacctgcgtgcaatccatcttgttcaatcatgcgaaacgatcctcatcctgtctcttgatccatggattacgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaggtcgggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttgatagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgccggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattaattcatgtaaaagatgaggttggttcattctcgaaaattacatctgtgttctcagagcggggcgtgacgtttgaaaaaatccttcagctgccaattaaaggccatgatgagttagctgaaatcgtaattgtcacacatcatacatcagaagctgatttcagtgatatcctgcaaaacctaaatgatttggaagtcgttcaagaagtcaaaagcacatatcgtgtagaagggaacggttggagctaatgtggaaaggacttatccatcaatataaagaatttttacctgtaacagatcaaacaccggcgctaactttacatgaaggaaacacacctcttattcacctgccgaagctgtctgagcagctcggaattgagcttcatgtcaaaacggaaggcgtcaatcctacgggatcatttaaagatcgcggaatggttatggctgtggcaaaggcaaaagaagaaggcaatgacacgattatgtgcgcgtcaacaggtaacacttccgctgctgcggcagcatatgcagcccgtgctaacatgaaatgcattgtcatcatcccgaacggaaaaattgcatttggaaaactcgctcaagctgtcatgtacggagccgagattatcgcaattgacggaaactttgacgatgcgcttaaaattgtccgttccatctgtgagaaatcaccgattgcccttgtcaactcagtcaacccttaccgcattgaaggccaaaaaactgctgccttcggatcagcgatatccacttcatccactccatttgtttaatctttaaattaagtatcaacatagtacatagcgaatcttccctttattatatctaatgtgttcataaaaaactaaaaaaaatattgaaaatactgacgaggttatataagatgaaaataagttagtttgtttaaacaacaaactaataggtgatgtacttactatatgaaataaaatgcatctgggatcccaagcttatcgaattcgcggccgcttctagag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1065204_sequence 1 aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtactagtagcggccgctgcaggataaaaaatttagaagccaatgaaatctataaataaactaaattaagtttatttaattaacaactatggatataaaataggtactaatcaaaatagtgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataaaaaaattgaaaaaatggtggaaacacttttttcaatttttttgttttattatttaatatttgggaaatattcattctaattggtaatcagattttagaaaacaataaacccttgcatagggggatctcgaggccttccgaaaatgcgcggctttgaagctgagggtgcggcggcaatcgtgcgcaatgaagtgattgaaaatccggaaacaatagcgacagccattcgtatcggaaacccggcaagctgggacaaagctgtaaaggcagccgaggaatccaatgggaaaattgacgaagtcactgatgatgaaatccttcacgcatatcagctgatcgcccgtgtagaaggcgtgtttgcagaaccaggttcttgcgcgtctatcgcaggagtgctgaaacaggtgaaatccggagaaattccgaaaggcagcaaggtcgtagctgtgttaacaggaaacggactgaaagatccgaacacagcggtcgacatttcagaaatcaagcctgtcacattgccgactgatgaagacagcatccttgaatatgtaaaaggagcggcccgtgtatgaacgaagccgacatgctgttctctgtcactgttcccggaagcacagctaacctaggccccggctttgattcagtcggaatggcgctcagcagatatttgaagctgaccgtctttgaaagcgacaaatggtcttttgaggctgaaacagaaacagtcgccggaattaattcgagctcctttaacttcctcaccagtagttgtatcggtaccataagtagaagcagcaacccaagtagctttaccagcatccggttcaaccagcatagtaagaatcttactggacatcggcagttcttcgaacagtgcgccaactaccagctctttctacagttcattcagggcaccggagaacctgcgtgcaatccatcttgttcaatcatgcgaaacgatcctcatcctgtctcttgatccatggattacgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaggtcgggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttgatagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgccggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattaattcatgtaaaagatgaggttggttcattctcgaaaattacatctgtgttctcagagcggggcgtgacgtttgaaaaaatccttcagctgccaattaaaggccatgatgagttagctgaaatcgtaattgtcacacatcatacatcagaagctgatttcagtgatatcctgcaaaacctaaatgatttggaagtcgttcaagaagtcaaaagcacatatcgtgtagaagggaacggttggagctaatgtggaaaggacttatccatcaatataaagaatttttacctgtaacagatcaaacaccggcgctaactttacatgaaggaaacacacctcttattcacctgccgaagctgtctgagcagctcggaattgagcttcatgtcaaaacggaaggcgtcaatcctacgggatcatttaaagatcgcggaatggttatggctgtggcaaaggcaaaagaagaaggcaatgacacgattatgtgcgcgtcaacaggtaacacttccgctgctgcggcagcatatgcagcccgtgctaacatgaaatgcattgtcatcatcccgaacggaaaaattgcatttggaaaactcgctcaagctgtcatgtacggagccgagattatcgcaattgacggaaactttgacgatgcgcttaaaattgtccgttccatctgtgagaaatcaccgattgcccttgtcaactcagtcaacccttaccgcattgaaggccaaaaaactgctgccttcggatcagcgatatccacttcatccactccatttgtttaatctttaaattaagtatcaacatagtacatagcgaatcttccctttattatatctaatgtgttcataaaaaactaaaaaaaatattgaaaatactgacgaggttatataagatgaaaataagttagtttgtttaaacaacaaactaataggtgatgtacttactatatgaaataaaatgcatctgggatcccaagcttatcgaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z