BBa_K1065204
1
xyl+efe
Efe+Bba_B0015 in BBa_K823024 (pXyl)
2013-08-31T11:00:00Z
2015-05-08T01:08:59Z
This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too.
2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pXyl vector that has been sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.
false
false
_1373_
0
15998
9
Not in stock
false
===Design Notes===
<html>
The coding sequence was taken from <I>Pseudomonas syringae</I> pv. <I>phaseolicola</I> PK2. We firstly optimized the codons for both <I>B. subtilis</I> and <I>E. coli</I> and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly.<br />
<br />
<span style= "color:brown" title="Prefix"><B>GAATTCGCGGCCGCTTCTAGA</B></span>TA<span style="color:purple; font-weight:bold" title="RBS">AGGAGG</span>AACTACT<b title="Start">ATG</b><span style="color:green" title="NgoMIV"><B>GCCGGC</B></span>ACC...<br/>
<br/>
...TCG<span style="color:red; font-weight:bold" title="AgeI">ACCGGT</span><b title="Stop">TAA</b><span style="color:blue; font-weight:bold" title="suffix">TACTAGTAGCGGCCGCTGCAG</span>
</html>
false
Viola Valentini
component2334267
1
BBa_K823024
component2334259
1
BBa_K1065002
annotation2334267
1
BBa_K823024
range2334267
1
1224
6017
annotation2334259
1
BBa_K1065002
range2334259
1
1
1215
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1065000
1
EFE
<html>2-oxoglutarate oxygenase/decarboxylase (EFE)</html>
2013-06-19T11:00:00Z
2015-05-08T01:08:59Z
xxxx
xxx
false
false
_1373_
0
15969
9
Not in stock
false
xxx
false
Thomas Perli
annotation2331673
1
BBa_K1065000
range2331673
1
1
1078
annotation2331676
1
Start
range2331676
1
14
16
annotation2331677
1
NgoMIV
range2331677
1
17
22
annotation2331679
1
Stop
range2331679
1
1076
1078
annotation2331675
1
CDS - Ethylene Forming Enzyme
range2331675
1
14
1078
annotation2331678
1
AgeI
range2331678
1
1070
1075
annotation2331672
1
RBS
range2331672
1
1
6
annotation2331674
1
Spacer
range2331674
1
7
13
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K823024
1
pSBBs4S
pSB<sub>Bs</sub>4S-P<i><sub>xyl</i></sub>: Integrative expression vector for <i>Bacillus subtilis</i
2012-08-20T11:00:00Z
2015-05-08T01:13:30Z
This part is an igem compatible version of the Bacillus expression vector pXT
This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose.
This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
false
false
_1081_
0
11555
9
It's complicated
false
We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
false
Jara Radeck
annotation2180243
1
bla
range2180243
1
2716
3576
annotation2180242
1
thrB
range2180242
1
1453
1863
annotation2180244
1
hom
range2180244
1
3731
4033
annotation2180245
1
thrC'
range2180245
1
4033
4572
annotation2180241
1
'thrC
range2180241
1
1028
1456
annotation2180240
1
spc
range2180240
1
211
885
annotation2180246
1
Pxly region
range2180246
1
4539
4763
BBa_K1065002
1
EFE + T
EFE + Terminators
2013-06-20T11:00:00Z
2015-05-08T01:08:59Z
c
BMST1 in psB1C3
false
false
_1373_
0
15968
9
In stock
true
c
false
Thomas Perli
component2331744
1
BBa_K1065000
component2331758
1
BBa_B0015
annotation2331758
1
BBa_B0015
range2331758
1
1087
1215
annotation2331744
1
BBa_K1065000
range2331744
1
1
1078
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1065000_sequence
1
aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaa
BBa_K1065002_sequence
1
aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K823024_sequence
1
tactagtagcggccgctgcaggataaaaaatttagaagccaatgaaatctataaataaactaaattaagtttatttaattaacaactatggatataaaataggtactaatcaaaatagtgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataaaaaaattgaaaaaatggtggaaacacttttttcaatttttttgttttattatttaatatttgggaaatattcattctaattggtaatcagattttagaaaacaataaacccttgcatagggggatctcgaggccttccgaaaatgcgcggctttgaagctgagggtgcggcggcaatcgtgcgcaatgaagtgattgaaaatccggaaacaatagcgacagccattcgtatcggaaacccggcaagctgggacaaagctgtaaaggcagccgaggaatccaatgggaaaattgacgaagtcactgatgatgaaatccttcacgcatatcagctgatcgcccgtgtagaaggcgtgtttgcagaaccaggttcttgcgcgtctatcgcaggagtgctgaaacaggtgaaatccggagaaattccgaaaggcagcaaggtcgtagctgtgttaacaggaaacggactgaaagatccgaacacagcggtcgacatttcagaaatcaagcctgtcacattgccgactgatgaagacagcatccttgaatatgtaaaaggagcggcccgtgtatgaacgaagccgacatgctgttctctgtcactgttcccggaagcacagctaacctaggccccggctttgattcagtcggaatggcgctcagcagatatttgaagctgaccgtctttgaaagcgacaaatggtcttttgaggctgaaacagaaacagtcgccggaattaattcgagctcctttaacttcctcaccagtagttgtatcggtaccataagtagaagcagcaacccaagtagctttaccagcatccggttcaaccagcatagtaagaatcttactggacatcggcagttcttcgaacagtgcgccaactaccagctctttctacagttcattcagggcaccggagaacctgcgtgcaatccatcttgttcaatcatgcgaaacgatcctcatcctgtctcttgatccatggattacgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaggtcgggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttgatagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgccggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattaattcatgtaaaagatgaggttggttcattctcgaaaattacatctgtgttctcagagcggggcgtgacgtttgaaaaaatccttcagctgccaattaaaggccatgatgagttagctgaaatcgtaattgtcacacatcatacatcagaagctgatttcagtgatatcctgcaaaacctaaatgatttggaagtcgttcaagaagtcaaaagcacatatcgtgtagaagggaacggttggagctaatgtggaaaggacttatccatcaatataaagaatttttacctgtaacagatcaaacaccggcgctaactttacatgaaggaaacacacctcttattcacctgccgaagctgtctgagcagctcggaattgagcttcatgtcaaaacggaaggcgtcaatcctacgggatcatttaaagatcgcggaatggttatggctgtggcaaaggcaaaagaagaaggcaatgacacgattatgtgcgcgtcaacaggtaacacttccgctgctgcggcagcatatgcagcccgtgctaacatgaaatgcattgtcatcatcccgaacggaaaaattgcatttggaaaactcgctcaagctgtcatgtacggagccgagattatcgcaattgacggaaactttgacgatgcgcttaaaattgtccgttccatctgtgagaaatcaccgattgcccttgtcaactcagtcaacccttaccgcattgaaggccaaaaaactgctgccttcggatcagcgatatccacttcatccactccatttgtttaatctttaaattaagtatcaacatagtacatagcgaatcttccctttattatatctaatgtgttcataaaaaactaaaaaaaatattgaaaatactgacgaggttatataagatgaaaataagttagtttgtttaaacaacaaactaataggtgatgtacttactatatgaaataaaatgcatctgggatcccaagcttatcgaattcgcggccgcttctagag
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1065204_sequence
1
aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtactagtagcggccgctgcaggataaaaaatttagaagccaatgaaatctataaataaactaaattaagtttatttaattaacaactatggatataaaataggtactaatcaaaatagtgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataaaaaaattgaaaaaatggtggaaacacttttttcaatttttttgttttattatttaatatttgggaaatattcattctaattggtaatcagattttagaaaacaataaacccttgcatagggggatctcgaggccttccgaaaatgcgcggctttgaagctgagggtgcggcggcaatcgtgcgcaatgaagtgattgaaaatccggaaacaatagcgacagccattcgtatcggaaacccggcaagctgggacaaagctgtaaaggcagccgaggaatccaatgggaaaattgacgaagtcactgatgatgaaatccttcacgcatatcagctgatcgcccgtgtagaaggcgtgtttgcagaaccaggttcttgcgcgtctatcgcaggagtgctgaaacaggtgaaatccggagaaattccgaaaggcagcaaggtcgtagctgtgttaacaggaaacggactgaaagatccgaacacagcggtcgacatttcagaaatcaagcctgtcacattgccgactgatgaagacagcatccttgaatatgtaaaaggagcggcccgtgtatgaacgaagccgacatgctgttctctgtcactgttcccggaagcacagctaacctaggccccggctttgattcagtcggaatggcgctcagcagatatttgaagctgaccgtctttgaaagcgacaaatggtcttttgaggctgaaacagaaacagtcgccggaattaattcgagctcctttaacttcctcaccagtagttgtatcggtaccataagtagaagcagcaacccaagtagctttaccagcatccggttcaaccagcatagtaagaatcttactggacatcggcagttcttcgaacagtgcgccaactaccagctctttctacagttcattcagggcaccggagaacctgcgtgcaatccatcttgttcaatcatgcgaaacgatcctcatcctgtctcttgatccatggattacgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaggtcgggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttgatagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgccggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattaattcatgtaaaagatgaggttggttcattctcgaaaattacatctgtgttctcagagcggggcgtgacgtttgaaaaaatccttcagctgccaattaaaggccatgatgagttagctgaaatcgtaattgtcacacatcatacatcagaagctgatttcagtgatatcctgcaaaacctaaatgatttggaagtcgttcaagaagtcaaaagcacatatcgtgtagaagggaacggttggagctaatgtggaaaggacttatccatcaatataaagaatttttacctgtaacagatcaaacaccggcgctaactttacatgaaggaaacacacctcttattcacctgccgaagctgtctgagcagctcggaattgagcttcatgtcaaaacggaaggcgtcaatcctacgggatcatttaaagatcgcggaatggttatggctgtggcaaaggcaaaagaagaaggcaatgacacgattatgtgcgcgtcaacaggtaacacttccgctgctgcggcagcatatgcagcccgtgctaacatgaaatgcattgtcatcatcccgaacggaaaaattgcatttggaaaactcgctcaagctgtcatgtacggagccgagattatcgcaattgacggaaactttgacgatgcgcttaaaattgtccgttccatctgtgagaaatcaccgattgcccttgtcaactcagtcaacccttaccgcattgaaggccaaaaaactgctgccttcggatcagcgatatccacttcatccactccatttgtttaatctttaaattaagtatcaacatagtacatagcgaatcttccctttattatatctaatgtgttcataaaaaactaaaaaaaatattgaaaatactgacgaggttatataagatgaaaataagttagtttgtttaaacaacaaactaataggtgatgtacttactatatgaaataaaatgcatctgggatcccaagcttatcgaattcgcggccgcttctagag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z