##gff-version 3
##sequence-region BBa_K1065204 1 6017
BBa_K1065204	.	engineered_region	1	1215	.	+	0	ID=BBa_K1065002;Name=BBa_K1065002
BBa_K1065204	.	terminator	1087	1215	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1065002
BBa_K1065204	.	terminator	1087	1166	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1065204	.	engineered_region	1087	1166	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1065204	.	stem_loop	1098	1141	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1065204	.	terminator	1175	1215	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1065204	.	stop_codon	1208	1208	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1065204	.	engineered_region	1175	1215	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1065204	.	polyA_site	1202	1215	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1065204	.	stem_loop	1182	1201	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1065204	.	engineered_region	1	1078	.	+	0	ID=BBa_K1065000;Name=BBa_K1065000;Parent=BBa_K1065002
BBa_K1065204	.	start_codon	14	16	.	+	0	ID=annotation2331676;Name=Start;Parent=BBa_K1065000
BBa_K1065204	.	sequence_feature	1070	1075	.	+	0	ID=annotation2331678;Name=AgeI;Parent=BBa_K1065000
BBa_K1065204	.	sequence_feature	7	13	.	+	0	ID=annotation2331674;Name=Spacer;Parent=BBa_K1065000
BBa_K1065204	.	CDS	14	1078	.	+	0	ID=annotation2331675;Name=CDS - Ethylene Forming Enzyme;Parent=BBa_K1065000
BBa_K1065204	.	stop_codon	1076	1078	.	+	0	ID=annotation2331679;Name=Stop;Parent=BBa_K1065000
BBa_K1065204	.	sequence_feature	17	22	.	+	0	ID=annotation2331677;Name=NgoMIV ;Parent=BBa_K1065000
BBa_K1065204	.	ribosome_entry_site	1	6	.	+	0	ID=annotation2331672;Name=RBS;Parent=BBa_K1065000
BBa_K1065204	.	engineered_region	1	1078	.	+	0	ID=annotation2331673;Name=BBa_K1065000;Parent=BBa_K1065000
BBa_K1065204	.	plasmid_vector	1224	6017	.	+	0	ID=BBa_K823024;Name=BBa_K823024
BBa_K1065204	.	sequence_feature	4954	5256	.	+	0	ID=annotation2180244;Name=hom;Parent=BBa_K823024
BBa_K1065204	.	CDS	1434	2108	.	+	0	ID=annotation2180240;Name=spc;Parent=BBa_K823024
BBa_K1065204	.	CDS	3939	4799	.	+	0	ID=annotation2180243;Name=bla;Parent=BBa_K823024
BBa_K1065204	.	sequence_feature	2676	3086	.	+	0	ID=annotation2180242;Name=thrB;Parent=BBa_K823024
BBa_K1065204	.	sequence_feature	5256	5795	.	+	0	ID=annotation2180245;Name=thrC';Parent=BBa_K823024
BBa_K1065204	.	sequence_feature	2251	2679	.	+	0	ID=annotation2180241;Name='thrC;Parent=BBa_K823024
BBa_K1065204	.	promoter	5762	5986	.	+	0	ID=annotation2180246;Name=Pxly region;Parent=BBa_K823024
>BBa_K1065204
aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatt
tcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgta
aaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatct
gacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgc
aaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacct
accaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggttt
cgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcag
accagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcg
gtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgtt
cgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaattt
atgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttcc
acgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactt
tacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctg
gaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtactagtagcggccgctgcaggataaaaaatttagaagcca
atgaaatctataaataaactaaattaagtttatttaattaacaactatggatataaaataggtactaatcaaaatagtg
aggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataacctta
ttggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatc
tgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagc
aacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaattta
tttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataat
gctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattt
tctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatat
taactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctga
atcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaat
gaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataaaaaaattgaaaaaatggtggaaaca
cttttttcaatttttttgttttattatttaatatttgggaaatattcattctaattggtaatcagattttagaaaacaa
taaacccttgcatagggggatctcgaggccttccgaaaatgcgcggctttgaagctgagggtgcggcggcaatcgtgcg
caatgaagtgattgaaaatccggaaacaatagcgacagccattcgtatcggaaacccggcaagctgggacaaagctgta
aaggcagccgaggaatccaatgggaaaattgacgaagtcactgatgatgaaatccttcacgcatatcagctgatcgccc
gtgtagaaggcgtgtttgcagaaccaggttcttgcgcgtctatcgcaggagtgctgaaacaggtgaaatccggagaaat
tccgaaaggcagcaaggtcgtagctgtgttaacaggaaacggactgaaagatccgaacacagcggtcgacatttcagaa
atcaagcctgtcacattgccgactgatgaagacagcatccttgaatatgtaaaaggagcggcccgtgtatgaacgaagc
cgacatgctgttctctgtcactgttcccggaagcacagctaacctaggccccggctttgattcagtcggaatggcgctc
agcagatatttgaagctgaccgtctttgaaagcgacaaatggtcttttgaggctgaaacagaaacagtcgccggaatta
attcgagctcctttaacttcctcaccagtagttgtatcggtaccataagtagaagcagcaacccaagtagctttaccag
catccggttcaaccagcatagtaagaatcttactggacatcggcagttcttcgaacagtgcgccaactaccagctcttt
ctacagttcattcagggcaccggagaacctgcgtgcaatccatcttgttcaatcatgcgaaacgatcctcatcctgtct
cttgatccatggattacgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaggtc
gggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg
gcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctg
ccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctca
gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccgg
taactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaga
gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggta
tctgcgctctgctgaagccagttaccttcggaaaaagagttgatagctcttgatccggcaaacaaaccaccgctggtag
cggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacg
gggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctaga
tccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgctt
aatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataact
acgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttat
cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaa
ttgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgccggcatcgtg
gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgt
tgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggt
tatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaag
tcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagca
gaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccag
ttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaaca
ggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatatt
attgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggt
tccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaatagg
cgtatcacgaggccctttcgtcttcaagaattaattcatgtaaaagatgaggttggttcattctcgaaaattacatctg
tgttctcagagcggggcgtgacgtttgaaaaaatccttcagctgccaattaaaggccatgatgagttagctgaaatcgt
aattgtcacacatcatacatcagaagctgatttcagtgatatcctgcaaaacctaaatgatttggaagtcgttcaagaa
gtcaaaagcacatatcgtgtagaagggaacggttggagctaatgtggaaaggacttatccatcaatataaagaattttt
acctgtaacagatcaaacaccggcgctaactttacatgaaggaaacacacctcttattcacctgccgaagctgtctgag
cagctcggaattgagcttcatgtcaaaacggaaggcgtcaatcctacgggatcatttaaagatcgcggaatggttatgg
ctgtggcaaaggcaaaagaagaaggcaatgacacgattatgtgcgcgtcaacaggtaacacttccgctgctgcggcagc
atatgcagcccgtgctaacatgaaatgcattgtcatcatcccgaacggaaaaattgcatttggaaaactcgctcaagct
gtcatgtacggagccgagattatcgcaattgacggaaactttgacgatgcgcttaaaattgtccgttccatctgtgaga
aatcaccgattgcccttgtcaactcagtcaacccttaccgcattgaaggccaaaaaactgctgccttcggatcagcgat
atccacttcatccactccatttgtttaatctttaaattaagtatcaacatagtacatagcgaatcttccctttattata
tctaatgtgttcataaaaaactaaaaaaaatattgaaaatactgacgaggttatataagatgaaaataagttagtttgt
ttaaacaacaaactaataggtgatgtacttactatatgaaataaaatgcatctgggatcccaagcttatcgaattcgcg
gccgcttctagag