LOCUS       BBa_K1065300            2967 bp    DNA     linear       05-Oct-2024
DEFINITION  R0010-YF1-FixJ-PfixK2-amilGFP
ACCESSION   BBa_K1065300
VERSION     BBa_K1065300.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1357
                     /label=YF1
     CDS             227..1357
                     /label=BBa_K592004
     misc_feature    1370..1373
                     /label=conserved
     RBS             1366..1377
                     /label=BBa_B0034
     CDS             1384..2004
                     /label=FixJ
     CDS             1384..2004
                     /label=BBa_K592005
     misc_feature    209..2004
                     /label=BBa_K592016
     promoter        2013..2262
                     /label=FixK2 promoter
     promoter        2013..2262
                     /label=BBa_K592006
     CDS             2269..2964
                     /label=amilGFP
     CDS             2269..2967
                     /label=BBa_K592010
     misc_feature    209..2967
                     /label=BBa_K952003
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctagttttca
      241 atcatttggg ataccaggac agctggaagt catcaaaaaa gcacttgatc acgtgcgagt
      301 cggtgtggta attacagatc ccgcacttga agataatcct attgtctacg taaatcaagg
      361 ctttgttcaa atgaccggct acgagaccga ggaaatttta ggaaagaact gtcgcttctt
      421 acaggggaaa cacacagatc cggcagaagt ggacaacatc agaaccgctt tacaaaataa
      481 agaaccggtc accgttcaga tccaaaacta caaaaaagac ggaacgatgt tctggaatga
      541 attaaatatt gatccaatgg aaatagagga taaaacgtat tttgtcggca ttcagaatga
      601 tatcaccgag caccagcaga cccaggcgcg tctccaggaa ctgcaatccg agctcgtcca
      661 cgtctccagg ctgagcgcca tgggcgaaat ggcgtccgcg ctcgcgcacg agctcaacca
      721 gccgctggcg gcgatcagca actacatgaa gggctcgcgg cggctgcttg ccggcagcag
      781 tgatccgaac acaccgaagg tcgaaagcgc cctggaccgc gccgccgagc aggcgctgcg
      841 cgccggccag atcatccggc gcctgcgcga cttcgttgcc cgcggcgaat cggagaagcg
      901 ggtcgagagt ctctccaagc tgatcgagga ggccggcgcg ctcgggcttg ccggcgcgcg
      961 cgagcagaac gtgcagctcc gcttcagtct cgatccgggc gccgatctcg ttctcgccga
     1021 ccgggtgcag atccagcagg tcctggtcaa cctgttccgc aacgcgctgg aagcgatggc
     1081 tcagtcgcag cgacgcgagc tcgtcgtcac caacaccccc gccgccgacg acatgatcga
     1141 ggtcgaagtg tccgacaccg gcagcggttt ccaggacgac gtcattccga acctgtttca
     1201 gactttcttc accaccaagg acaccggcat gggcgtggga ctgtccatca gccgctcgat
     1261 catcgaagct cacggcgggc gcatgtgggc cgagagcaac gcatcgggcg gggcgacctt
     1321 ccgcttcacc ctcccggcag ccgacgagaa ttaataatac tagagaaaga ggagaaatac
     1381 tagatgacga ccaagggaca tatctacgtc atcgacgacg acgcggcgat gcgggattcg
     1441 ctgaatttcc tgctggattc tgccggcttc ggcgtcacgc tgtttgacga cgcgcaagcc
     1501 tttctcgacg ccctgccggg tctctccttc ggctgcgtcg tctccgacgt gcgcatgccg
     1561 ggccttgacg gcatcgagct gttgaagcgg atgaaggcgc agcaaagccc ctttccgatc
     1621 ctcatcatga ccggtcacgg cgacgtgccg ctcgcggtcg aggcgatgaa gttaggggcg
     1681 gtcgactttc tggaaaagcc tttcgaggac gaccgcctca ccgccatgat cgaatcggcg
     1741 atccgccagg ccgagccggc cgccaagagc gaggccgtcg cgcaggatat cgccgcccgc
     1801 gtcgcctcgt tgagccccag ggagcgccag gtcatggaag ggctgatcgc cggcctttcc
     1861 aacaagctga tcgcccgcga gtacgacatc agcccgcgca ccatcgaggt gtatcgggcc
     1921 aacgtcatga ccaagatgca ggccaacagc ctttcggagc tggttcgcct cgcgatgcgc
     1981 gccggcatgc tcaacgatta ataatactag aggccggagc cgattatccg cacccgtcct
     2041 tggtcttgga cacactcgct cgcgatccga agccgccttt caaagatact ccgcagtgaa
     2101 acgcatcttt taagcgcgac ttgtcgcctt cgcgcagcag aacactcgta tggcactcaa
     2161 tccatgatcg cttcgttcgc tccgacgcgc gttgcggggc ccccctcgac cggatgacaa
     2221 cacattgcgt cacctcaact gctgcgcgcg cactgcgtca catactagat gtcttattca
     2281 aagcatggca tcgtacaaga aatgaagacg aaataccata tggaaggcag tgtcaatggc
     2341 catgaattta cgatcgaagg tgtaggaact gggtaccctt acgaagggaa acagatgtcc
     2401 gaattagtga tcatcaagcc tgcgggaaaa ccccttccat tctcctttga catactgtca
     2461 tcagtctttc aatatggaaa ccgttgcttc acaaagtacc cggcagacat gcctgactat
     2521 ttcaagcaag cattcccaga tggaatgtca tatgaaaggt catttctatt tgaggatgga
     2581 gcagttgcta cagccagctg gaacattcgt ctcgaaggaa attgcttcat ccacaaatcc
     2641 atctttcatg gcgtaaactt tcccgctgat ggacccgtaa tgaaaaagaa gacaattgac
     2701 tgggataagt ccttcgaaaa aatgactgtg tctaaagagg tgctaagagg tgacgtgact
     2761 atgtttctta tgctcgaagg aggtggttct cacagatgcc aatttcactc cacttacaaa
     2821 acagagaagc cggtcacact gcccccgaat catgtcgtag aacatcaaat tgtgaggacc
     2881 gaccttggcc aaagtgcaaa aggctttaca gtcaagctgg aagcacatgc cgcggctcat
     2941 gttaaccctt tgaaggttaa ataataa
//