R0010 derived from the registry, at the same K952003 derived from the registry
This part is composed by a pLacI and K952003. The part don't work because is laking RBS between PfixK2 and amilGFP.
I created the part inserting R0010 in the front of K952003 device.
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
BBa_K592016, BBa_K592006, and BBa_K592010 are available in the parts registry. They were obtained directly from the Uppsala-Sweden 2011 team.
This part is a composite of BBa_K592016, BBa_K592006, and BBa_K592010 in that order. When the cells are exposed to blue-light, they will express amilGFP, a yellow chromoprotein. This protein causes the cells to have a strong yellow color.
FixK2 promoter omes from the genome of Bradyrhizobium japonicum.
FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.
The sequence information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
This chromoprotein, amilGFP, naturally exhibits very strong yellow color when expressed. The color is strong and readily visible to naked eye both in LB-culture and on agar plates. The DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of two illegal internal restriction sites (EcoRI and PstI).
Two illegal internal restriction sites (EcoRI and PstI) was removed.
B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS
The components that made up of YF1 fusion protein comes from Bradyrhizobium japonicum and Bacillus subtilis. FixJ comes from the genome of Bradyrhizobium japonicum.
YF1 works in conjunction with its response regulator, FixJ. This part consists of RBS-coupled YF1 and FixJ without promoter.
This constructs Strong RBS to ensure good expression.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Ytva comes from the genome of Bacillus subtilis. FixL derives from the genome of Bradyrhizobium japonicum.
YF1 is a fusion protein of YtvA (B.subtilis) and FixL(Br.japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. As shown in previous research, Illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo.
The sequence information was provided in Moglich and Moffat's article. The physical DNA was synthesized by GenScript. Codon-optimization may enhance its expression.
FixJ comes from the genome of Bradyrhizobium japonicum.
FixJ comes from the genome of Bradyrhizobium japonicum. It's the wild-type response regulator to YF1, the blue light-sensing fusion protein.
The sequence was information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
Conversion of the iGEM parts registry to SBOL2.1