LOCUS       BBa_K1065302            2982 bp    DNA     linear       22-Nov-2024
DEFINITION  Blue light sensing device without inverter for the production of 
            amilGFP
ACCESSION   BBa_K1065302
VERSION     BBa_K1065302.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..220
                     /label=BBa_B0034
     misc_feature    209..2004
                     /label=BBa_K592016
     misc_feature    209..2982
                     /label=BBa_K1065305
     misc_feature    213..216
                     /label=conserved
     misc_feature    227..1357
                     /label=BBa_K592004
     CDS             227..1357
                     /label=YF1
     misc_feature    1366..1377
                     /label=BBa_B0034
     misc_feature    1370..1373
                     /label=conserved
     CDS             1384..2004
                     /label=FixJ
     misc_feature    1384..2004
                     /label=BBa_K592005
     promoter        2013..2262
                     /label=FixK2 promoter
     misc_feature    2013..2262
                     /label=BBa_K592006
     RBS             2269..2283
                     /label=RBS: gaggaggataatct
     misc_feature    2284..2982
                     /label=BBa_K592010
     CDS             2284..2982
                     /label=amilGFP
     misc_feature    209..2982
                     /label=BBa_K1065305
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctagttttca
      241 atcatttggg ataccaggac agctggaagt catcaaaaaa gcacttgatc acgtgcgagt
      301 cggtgtggta attacagatc ccgcacttga agataatcct attgtctacg taaatcaagg
      361 ctttgttcaa atgaccggct acgagaccga ggaaatttta ggaaagaact gtcgcttctt
      421 acaggggaaa cacacagatc cggcagaagt ggacaacatc agaaccgctt tacaaaataa
      481 agaaccggtc accgttcaga tccaaaacta caaaaaagac ggaacgatgt tctggaatga
      541 attaaatatt gatccaatgg aaatagagga taaaacgtat tttgtcggca ttcagaatga
      601 tatcaccgag caccagcaga cccaggcgcg tctccaggaa ctgcaatccg agctcgtcca
      661 cgtctccagg ctgagcgcca tgggcgaaat ggcgtccgcg ctcgcgcacg agctcaacca
      721 gccgctggcg gcgatcagca actacatgaa gggctcgcgg cggctgcttg ccggcagcag
      781 tgatccgaac acaccgaagg tcgaaagcgc cctggaccgc gccgccgagc aggcgctgcg
      841 cgccggccag atcatccggc gcctgcgcga cttcgttgcc cgcggcgaat cggagaagcg
      901 ggtcgagagt ctctccaagc tgatcgagga ggccggcgcg ctcgggcttg ccggcgcgcg
      961 cgagcagaac gtgcagctcc gcttcagtct cgatccgggc gccgatctcg ttctcgccga
     1021 ccgggtgcag atccagcagg tcctggtcaa cctgttccgc aacgcgctgg aagcgatggc
     1081 tcagtcgcag cgacgcgagc tcgtcgtcac caacaccccc gccgccgacg acatgatcga
     1141 ggtcgaagtg tccgacaccg gcagcggttt ccaggacgac gtcattccga acctgtttca
     1201 gactttcttc accaccaagg acaccggcat gggcgtggga ctgtccatca gccgctcgat
     1261 catcgaagct cacggcgggc gcatgtgggc cgagagcaac gcatcgggcg gggcgacctt
     1321 ccgcttcacc ctcccggcag ccgacgagaa ttaataatac tagagaaaga ggagaaatac
     1381 tagatgacga ccaagggaca tatctacgtc atcgacgacg acgcggcgat gcgggattcg
     1441 ctgaatttcc tgctggattc tgccggcttc ggcgtcacgc tgtttgacga cgcgcaagcc
     1501 tttctcgacg ccctgccggg tctctccttc ggctgcgtcg tctccgacgt gcgcatgccg
     1561 ggccttgacg gcatcgagct gttgaagcgg atgaaggcgc agcaaagccc ctttccgatc
     1621 ctcatcatga ccggtcacgg cgacgtgccg ctcgcggtcg aggcgatgaa gttaggggcg
     1681 gtcgactttc tggaaaagcc tttcgaggac gaccgcctca ccgccatgat cgaatcggcg
     1741 atccgccagg ccgagccggc cgccaagagc gaggccgtcg cgcaggatat cgccgcccgc
     1801 gtcgcctcgt tgagccccag ggagcgccag gtcatggaag ggctgatcgc cggcctttcc
     1861 aacaagctga tcgcccgcga gtacgacatc agcccgcgca ccatcgaggt gtatcgggcc
     1921 aacgtcatga ccaagatgca ggccaacagc ctttcggagc tggttcgcct cgcgatgcgc
     1981 gccggcatgc tcaacgatta ataatactag aggccggagc cgattatccg cacccgtcct
     2041 tggtcttgga cacactcgct cgcgatccga agccgccttt caaagatact ccgcagtgaa
     2101 acgcatcttt taagcgcgac ttgtcgcctt cgcgcagcag aacactcgta tggcactcaa
     2161 tccatgatcg cttcgttcgc tccgacgcgc gttgcggggc ccccctcgac cggatgacaa
     2221 cacattgcgt cacctcaact gctgcgcgcg cactgcgtca catactagag aggaggataa
     2281 tctatgtctt attcaaagca tggcatcgta caagaaatga agacgaaata ccatatggaa
     2341 ggcagtgtca atggccatga atttacgatc gaaggtgtag gaactgggta cccttacgaa
     2401 gggaaacaga tgtccgaatt agtgatcatc aagcctgcgg gaaaacccct tccattctcc
     2461 tttgacatac tgtcatcagt ctttcaatat ggaaaccgtt gcttcacaaa gtacccggca
     2521 gacatgcctg actatttcaa gcaagcattc ccagatggaa tgtcatatga aaggtcattt
     2581 ctatttgagg atggagcagt tgctacagcc agctggaaca ttcgtctcga aggaaattgc
     2641 ttcatccaca aatccatctt tcatggcgta aactttcccg ctgatggacc cgtaatgaaa
     2701 aagaagacaa ttgactggga taagtccttc gaaaaaatga ctgtgtctaa agaggtgcta
     2761 agaggtgacg tgactatgtt tcttatgctc gaaggaggtg gttctcacag atgccaattt
     2821 cactccactt acaaaacaga gaagccggtc acactgcccc cgaatcatgt cgtagaacat
     2881 caaattgtga ggaccgacct tggccaaagt gcaaaaggct ttacagtcaa gctggaagca
     2941 catgccgcgg ctcatgttaa ccctttgaag gttaaataat aa
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