LOCUS       BBa_K1065307            1976 bp    DNA     linear       15-Dec-2024
DEFINITION  J23100+YF1+FixJ+terminator
ACCESSION   BBa_K1065307
VERSION     BBa_K1065307.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1192
                     /label=YF1
     CDS             62..1192
                     /label=BBa_K592004
     misc_feature    1205..1208
                     /label=conserved
     RBS             1201..1212
                     /label=BBa_B0034
     CDS             1219..1839
                     /label=FixJ
     CDS             1219..1839
                     /label=BBa_K592005
     misc_feature    44..1839
                     /label=BBa_K592016
     misc_feature    1..1839
                     /label=BBa_K1065306
     stem_loop       1859..1902
                     /label=stem_loop
     misc_feature    1848..1927
                     /label=BBa_B0010
     terminator      1848..1927
                     /label=BBa_B0010
     stem_loop       1943..1962
                     /label=T7 TE
     misc_feature    1969..1969
                     /label=stop
     polyA_site      1963..1976
                     /label=polya
     misc_feature    1936..1976
                     /label=BBa_B0012
     terminator      1936..1976
                     /label=BBa_B0012
     terminator      1848..1976
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggctagt tttcaatcat ttgggatacc aggacagctg gaagtcatca aaaaagcact
      121 tgatcacgtg cgagtcggtg tggtaattac agatcccgca cttgaagata atcctattgt
      181 ctacgtaaat caaggctttg ttcaaatgac cggctacgag accgaggaaa ttttaggaaa
      241 gaactgtcgc ttcttacagg ggaaacacac agatccggca gaagtggaca acatcagaac
      301 cgctttacaa aataaagaac cggtcaccgt tcagatccaa aactacaaaa aagacggaac
      361 gatgttctgg aatgaattaa atattgatcc aatggaaata gaggataaaa cgtattttgt
      421 cggcattcag aatgatatca ccgagcacca gcagacccag gcgcgtctcc aggaactgca
      481 atccgagctc gtccacgtct ccaggctgag cgccatgggc gaaatggcgt ccgcgctcgc
      541 gcacgagctc aaccagccgc tggcggcgat cagcaactac atgaagggct cgcggcggct
      601 gcttgccggc agcagtgatc cgaacacacc gaaggtcgaa agcgccctgg accgcgccgc
      661 cgagcaggcg ctgcgcgccg gccagatcat ccggcgcctg cgcgacttcg ttgcccgcgg
      721 cgaatcggag aagcgggtcg agagtctctc caagctgatc gaggaggccg gcgcgctcgg
      781 gcttgccggc gcgcgcgagc agaacgtgca gctccgcttc agtctcgatc cgggcgccga
      841 tctcgttctc gccgaccggg tgcagatcca gcaggtcctg gtcaacctgt tccgcaacgc
      901 gctggaagcg atggctcagt cgcagcgacg cgagctcgtc gtcaccaaca cccccgccgc
      961 cgacgacatg atcgaggtcg aagtgtccga caccggcagc ggtttccagg acgacgtcat
     1021 tccgaacctg tttcagactt tcttcaccac caaggacacc ggcatgggcg tgggactgtc
     1081 catcagccgc tcgatcatcg aagctcacgg cgggcgcatg tgggccgaga gcaacgcatc
     1141 gggcggggcg accttccgct tcaccctccc ggcagccgac gagaattaat aatactagag
     1201 aaagaggaga aatactagat gacgaccaag ggacatatct acgtcatcga cgacgacgcg
     1261 gcgatgcggg attcgctgaa tttcctgctg gattctgccg gcttcggcgt cacgctgttt
     1321 gacgacgcgc aagcctttct cgacgccctg ccgggtctct ccttcggctg cgtcgtctcc
     1381 gacgtgcgca tgccgggcct tgacggcatc gagctgttga agcggatgaa ggcgcagcaa
     1441 agcccctttc cgatcctcat catgaccggt cacggcgacg tgccgctcgc ggtcgaggcg
     1501 atgaagttag gggcggtcga ctttctggaa aagcctttcg aggacgaccg cctcaccgcc
     1561 atgatcgaat cggcgatccg ccaggccgag ccggccgcca agagcgaggc cgtcgcgcag
     1621 gatatcgccg cccgcgtcgc ctcgttgagc cccagggagc gccaggtcat ggaagggctg
     1681 atcgccggcc tttccaacaa gctgatcgcc cgcgagtacg acatcagccc gcgcaccatc
     1741 gaggtgtatc gggccaacgt catgaccaag atgcaggcca acagcctttc ggagctggtt
     1801 cgcctcgcga tgcgcgccgg catgctcaac gattaataat actagagcca ggcatcaaat
     1861 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1921 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//