LOCUS       BBa_K1065308            3092 bp    DNA     linear       15-Nov-2024
DEFINITION  J23100+YF1+FixJ+FixK2+inverter
ACCESSION   BBa_K1065308
VERSION     BBa_K1065308.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1192
                     /label=YF1
     CDS             62..1192
                     /label=BBa_K592004
     misc_feature    1205..1208
                     /label=conserved
     RBS             1201..1212
                     /label=BBa_B0034
     CDS             1219..1839
                     /label=FixJ
     CDS             1219..1839
                     /label=BBa_K592005
     misc_feature    44..1839
                     /label=BBa_K592016
     misc_feature    1..1839
                     /label=BBa_K1065306
     promoter        1848..2097
                     /label=FixK2 promoter
     promoter        1848..2097
                     /label=BBa_K592006
     misc_feature    2110..2113
                     /label=conserved
     RBS             2106..2117
                     /label=BBa_B0034
     CDS             2127..2834
                     /label=cI lambda
     misc_feature    2835..2867
                     /label=LVA
     misc_feature    2874..2898
                     /label=Help:Barcodes
     CDS             2124..2873
                     /label=BBa_C0051
     stem_loop       2918..2961
                     /label=stem_loop
     misc_feature    2907..2986
                     /label=BBa_B0010
     terminator      2907..2986
                     /label=BBa_B0010
     stem_loop       3002..3021
                     /label=T7 TE
     misc_feature    3028..3028
                     /label=stop
     polyA_site      3022..3035
                     /label=polya
     misc_feature    2995..3035
                     /label=BBa_B0012
     terminator      2995..3035
                     /label=BBa_B0012
     promoter        3081..3086
                     /label=-10
     promoter        3058..3063
                     /label=-35
     misc_feature    3068..3084
                     /label=OR1
     misc_feature    3044..3060
                     /label=OR2
     misc_feature    3044..3092
                     /label=BBa_R0051
     promoter        3044..3092
                     /label=BBa_R0051
     misc_feature    2106..3092
                     /label=BBa_Q04510
     misc_feature    1848..3092
                     /label=BBa_S04617
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggctagt tttcaatcat ttgggatacc aggacagctg gaagtcatca aaaaagcact
      121 tgatcacgtg cgagtcggtg tggtaattac agatcccgca cttgaagata atcctattgt
      181 ctacgtaaat caaggctttg ttcaaatgac cggctacgag accgaggaaa ttttaggaaa
      241 gaactgtcgc ttcttacagg ggaaacacac agatccggca gaagtggaca acatcagaac
      301 cgctttacaa aataaagaac cggtcaccgt tcagatccaa aactacaaaa aagacggaac
      361 gatgttctgg aatgaattaa atattgatcc aatggaaata gaggataaaa cgtattttgt
      421 cggcattcag aatgatatca ccgagcacca gcagacccag gcgcgtctcc aggaactgca
      481 atccgagctc gtccacgtct ccaggctgag cgccatgggc gaaatggcgt ccgcgctcgc
      541 gcacgagctc aaccagccgc tggcggcgat cagcaactac atgaagggct cgcggcggct
      601 gcttgccggc agcagtgatc cgaacacacc gaaggtcgaa agcgccctgg accgcgccgc
      661 cgagcaggcg ctgcgcgccg gccagatcat ccggcgcctg cgcgacttcg ttgcccgcgg
      721 cgaatcggag aagcgggtcg agagtctctc caagctgatc gaggaggccg gcgcgctcgg
      781 gcttgccggc gcgcgcgagc agaacgtgca gctccgcttc agtctcgatc cgggcgccga
      841 tctcgttctc gccgaccggg tgcagatcca gcaggtcctg gtcaacctgt tccgcaacgc
      901 gctggaagcg atggctcagt cgcagcgacg cgagctcgtc gtcaccaaca cccccgccgc
      961 cgacgacatg atcgaggtcg aagtgtccga caccggcagc ggtttccagg acgacgtcat
     1021 tccgaacctg tttcagactt tcttcaccac caaggacacc ggcatgggcg tgggactgtc
     1081 catcagccgc tcgatcatcg aagctcacgg cgggcgcatg tgggccgaga gcaacgcatc
     1141 gggcggggcg accttccgct tcaccctccc ggcagccgac gagaattaat aatactagag
     1201 aaagaggaga aatactagat gacgaccaag ggacatatct acgtcatcga cgacgacgcg
     1261 gcgatgcggg attcgctgaa tttcctgctg gattctgccg gcttcggcgt cacgctgttt
     1321 gacgacgcgc aagcctttct cgacgccctg ccgggtctct ccttcggctg cgtcgtctcc
     1381 gacgtgcgca tgccgggcct tgacggcatc gagctgttga agcggatgaa ggcgcagcaa
     1441 agcccctttc cgatcctcat catgaccggt cacggcgacg tgccgctcgc ggtcgaggcg
     1501 atgaagttag gggcggtcga ctttctggaa aagcctttcg aggacgaccg cctcaccgcc
     1561 atgatcgaat cggcgatccg ccaggccgag ccggccgcca agagcgaggc cgtcgcgcag
     1621 gatatcgccg cccgcgtcgc ctcgttgagc cccagggagc gccaggtcat ggaagggctg
     1681 atcgccggcc tttccaacaa gctgatcgcc cgcgagtacg acatcagccc gcgcaccatc
     1741 gaggtgtatc gggccaacgt catgaccaag atgcaggcca acagcctttc ggagctggtt
     1801 cgcctcgcga tgcgcgccgg catgctcaac gattaataat actagaggcc ggagccgatt
     1861 atccgcaccc gtccttggtc ttggacacac tcgctcgcga tccgaagccg cctttcaaag
     1921 atactccgca gtgaaacgca tcttttaagc gcgacttgtc gccttcgcgc agcagaacac
     1981 tcgtatggca ctcaatccat gatcgcttcg ttcgctccga cgcgcgttgc ggggcccccc
     2041 tcgaccggat gacaacacat tgcgtcacct caactgctgc gcgcgcactg cgtcacatac
     2101 tagagaaaga ggagaaatac tagatgagca caaaaaagaa accattaaca caagagcagc
     2161 ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat
     2221 cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt gctttattta
     2281 atggcatcaa tgcattaaat gcttataacg ccgcattgct tgcaaaaatt ctcaaagtta
     2341 gcgttgaaga atttagccct tcaatcgcca gagaaatcta cgagatgtat gaagcggtta
     2401 gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat gttcaggcag
     2461 ggatgttctc acctgagctt agaaccttta ccaaaggtga tgcggagaga tgggtaagca
     2521 caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat tccatgaccg
     2581 caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt gaccctgagc
     2641 aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag tttaccttca
     2701 agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca cagtacccaa
     2761 tgatcccatg caatgagagt tgttccgttg tggggaaagt tatcgctagt cagtggcctg
     2821 aagagacgtt tggcgctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat
     2881 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     2941 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     3001 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtaacacc gtgcgtgttg
     3061 actattttac ctctggcggt gataatggtt gc
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