##gff-version 3
##sequence-region BBa_K1065309 1 4205
BBa_K1065309	.	engineered_region	2991	4205	.	+	0	ID=BBa_K1065002;Name=BBa_K1065002
BBa_K1065309	.	engineered_region	2991	4068	.	+	0	ID=BBa_K1065000;Name=BBa_K1065000;Parent=BBa_K1065002
BBa_K1065309	.	stop_codon	4066	4068	.	+	0	ID=annotation2331679;Name=Stop;Parent=BBa_K1065000
BBa_K1065309	.	engineered_region	2991	4068	.	+	0	ID=annotation2331673;Name=BBa_K1065000;Parent=BBa_K1065000
BBa_K1065309	.	sequence_feature	2997	3003	.	+	0	ID=annotation2331674;Name=Spacer;Parent=BBa_K1065000
BBa_K1065309	.	start_codon	3004	3006	.	+	0	ID=annotation2331676;Name=Start;Parent=BBa_K1065000
BBa_K1065309	.	sequence_feature	3007	3012	.	+	0	ID=annotation2331677;Name=NgoMIV ;Parent=BBa_K1065000
BBa_K1065309	.	CDS	3004	4068	.	+	0	ID=annotation2331675;Name=CDS - Ethylene Forming Enzyme;Parent=BBa_K1065000
BBa_K1065309	.	sequence_feature	4060	4065	.	+	0	ID=annotation2331678;Name=AgeI;Parent=BBa_K1065000
BBa_K1065309	.	ribosome_entry_site	2991	2996	.	+	0	ID=annotation2331672;Name=RBS;Parent=BBa_K1065000
BBa_K1065309	.	terminator	4077	4205	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1065002
BBa_K1065309	.	terminator	4077	4156	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1065309	.	engineered_region	4077	4156	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1065309	.	stem_loop	4088	4131	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1065309	.	terminator	4165	4205	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1065309	.	engineered_region	4165	4205	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1065309	.	stop_codon	4198	4198	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1065309	.	stem_loop	4172	4191	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1065309	.	polyA_site	4192	4205	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1065309	.	engineered_region	1	2982	.	+	0	ID=BBa_K1065302;Name=BBa_K1065302
BBa_K1065309	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1065302
BBa_K1065309	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1065309	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1065309	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1065309	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1065309	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1065309	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1065309	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1065309	.	engineered_region	209	2982	.	+	0	ID=BBa_K1065305;Name=BBa_K1065305;Parent=BBa_K1065302
BBa_K1065309	.	conserved_region	213	216	.	+	0	ID=annotation2368303;Name=conserved;Parent=BBa_K1065305
BBa_K1065309	.	engineered_region	2013	2262	.	+	0	ID=annotation2368311;Name=BBa_K592006;Parent=BBa_K1065305
BBa_K1065309	.	engineered_region	209	2004	.	+	0	ID=annotation2368301;Name=BBa_K592016;Parent=BBa_K1065305
BBa_K1065309	.	CDS	1384	2004	.	+	0	ID=annotation2368308;Name=FixJ;Parent=BBa_K1065305
BBa_K1065309	.	engineered_region	2284	2982	.	+	0	ID=annotation2368313;Name=BBa_K592010;Parent=BBa_K1065305
BBa_K1065309	.	engineered_region	1384	2004	.	+	0	ID=annotation2368309;Name=BBa_K592005;Parent=BBa_K1065305
BBa_K1065309	.	engineered_region	227	1357	.	+	0	ID=annotation2368304;Name=BBa_K592004;Parent=BBa_K1065305
BBa_K1065309	.	engineered_region	209	2982	.	+	0	ID=annotation2368302;Name=BBa_K1065305;Parent=BBa_K1065305
BBa_K1065309	.	CDS	2284	2982	.	+	0	ID=annotation2368314;Name=amilGFP;Parent=BBa_K1065305
BBa_K1065309	.	ribosome_entry_site	2269	2283	.	+	0	ID=annotation2368312;Name=RBS: gaggaggataatct;Parent=BBa_K1065305
BBa_K1065309	.	CDS	227	1357	.	+	0	ID=annotation2368305;Name=YF1;Parent=BBa_K1065305
BBa_K1065309	.	engineered_region	209	220	.	+	0	ID=annotation2368300;Name=BBa_B0034;Parent=BBa_K1065305
BBa_K1065309	.	conserved_region	1370	1373	.	+	0	ID=annotation2368307;Name=conserved;Parent=BBa_K1065305
BBa_K1065309	.	promoter	2013	2262	.	+	0	ID=annotation2368310;Name=FixK2 promoter;Parent=BBa_K1065305
BBa_K1065309	.	engineered_region	1366	1377	.	+	0	ID=annotation2368306;Name=BBa_B0034;Parent=BBa_K1065305
>BBa_K1065309
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggctagttt
tcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattaca
gatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaa
ttttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttaca
aaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgat
ccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtc
tccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacga
gctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaac
acaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcg
acttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggct
tgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtg
cagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcg
tcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcat
tccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatc
gaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccg
acgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgac
gcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcct
ttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagct
gttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtc
gaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcgg
cgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccc
cagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccg
cgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcga
tgcgcgccggcatgctcaacgattaataatactagaggccggagccgattatccgcacccgtccttggtcttggacaca
ctcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgc
gcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccg
gatgacaacacattgcgtcacctcaactgctgcgcgcgcactgcgtcacatactagagaggaggataatctatgtctta
ttcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatc
gaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaacccc
ttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcc
tgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctaca
gccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatg
gacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagagg
tgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaag
ccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggcttta
cagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaataataatactagagaggaggaactac
tatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgt
gccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaag
ccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcagg
atacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtca
gtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagca
tgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgat
taatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactg
tcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattc
gtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatga
accgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggc
caactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaact
ttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgtt
catgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaa
aaatactcggacacaagagcaactggttcgaccggttaatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata