light regulated circuit producing amilGFP+EFE at dark
the part sources are: Bba_K1065302(our improved part) and BBa_K1065002 (EFE+terminators), both submitted in the registry by our team)
This part includes blue light sensor YF1, its RR fixJ,PfixK2,the reporter amilGFP and EFE+terminators: everything is under the regulation of Plac. under blue light fixJ is phosphorilated and inhibits the activity of Pfixk2 consisting in production of amilGFP+EFE only at dark.
this part doesn't present the inverter cassette, so transcription is activated at dark.
Blue light sensing device without inverter for the production of amilGFP
The part come from a BioBrick already present in the registry
In the absence of the blue-light the cell express amilGFP. In the presence of blue-light the cell don't produce amilGFP.
I create this parts insert promoter lacI regulated (BBa_R0010) at the part BBa_K952003 with insertion of RBS (between FixK2 promoter and amilGFP) with mutagenesis
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
Double terminator consisting of BBa_B0010 and BBa_B0012
2-oxoglutarate oxygenase/decarboxylase (EFE)
Blue light sensor without inverter for the production of amilGFP
The part potentially produce amilGFP in the dark (without the blue-light) when assembly with a promoter
Modify the already exist sequence, inserting a RBS between PfixK2 and amilGFP to produce the protein
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1