BBa_K1065309
type: ComponentDefinition
persistent identity: BBa K1065309
display id: BBa_K1065309
version: 1
was generated by: iGEM to SBOL conversion
description: light regulated circuit producing amilGFP+EFE at dark
created: 2013-09-17T11:00:00Z
modified: 2015-05-08T01:08:59Z
mutable provenance: the part sources are: Bba_K1065302(our improved part) and BBa_K1065002 (EFE+terminators), both submitted in the registry by our team)
owned by: james
owned by: myers
mutable description: This part includes blue light sensor YF1, its RR fixJ,PfixK2,the reporter amilGFP and EFE+terminators: everything is under the regulation of Plac. under blue light fixJ is phosphorilated and inhibits the activity of Pfixk2 consisting in production of amilGFP+EFE only at dark.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1373_
m user id: 0
owner id: 16023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: this part doesn't present the inverter cassette, so transcription is activated at dark.
star: false
creator: Fabio Digiacomo
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K1065002
component: BBa_K1065302
sequence annotation: BBa_K1065302
sequence annotation: BBa_K1065002
sequence: https://synbiohub.org/public/igem/BBa_K1065309_sequence/1

BBa_K1065002
type: Component
persistent identity: component2352551
display id: component2352551
version: 1
top level: BBa_K1065309
definition: EFE + T
access: public

BBa_K1065302
type: Component
persistent identity: component2352535
display id: component2352535
version: 1
top level: BBa_K1065309
definition: BBa_K1065302
access: public

BBa_K1065302
type: SequenceAnnotation
persistent identity: annotation2352535
display id: annotation2352535
version: 1
top level: BBa_K1065309
location: https://synbiohub.org/public/igem/BBa_K1065309/annotation2352535/range2352535/1
component: BBa_K1065302

https://synbiohub.org/public/igem/BBa_K1065309/annotation2352535/range2352535/1
type: Range
persistent identity: range2352535
display id: range2352535
version: 1
top level: BBa_K1065309
direction: direction/reverse
start: 1
end: 2982
orientation: inline

BBa_K1065002
type: SequenceAnnotation
persistent identity: annotation2352551
display id: annotation2352551
version: 1
top level: BBa_K1065309
location: https://synbiohub.org/public/igem/BBa_K1065309/annotation2352551/range2352551/1
component: BBa_K1065002

https://synbiohub.org/public/igem/BBa_K1065309/annotation2352551/range2352551/1
type: Range
persistent identity: range2352551
display id: range2352551
version: 1
top level: BBa_K1065309
direction: direction/reverse
start: 2991
end: 4205
orientation: inline

EFE + T
type: ComponentDefinition
persistent identity: BBa K1065002
display id: BBa_K1065002
version: 1
was generated by: iGEM to SBOL conversion
description: EFE + Terminators
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:08:59Z
mutable provenance: c
owned by: james
owned by: myers
mutable description: BMST1 in psB1C3
discontinued: false
dominant: false
experience: experience/Works
group u list: _1373_
m user id: 0
owner id: 15968
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: c
star: false
creator: Thomas Perli
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K1065000
sequence annotation: BBa_K1065000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1065002_sequence/1

BBa_B0015
type: Component
persistent identity: component2331758
display id: component2331758
version: 1
top level: EFE + T
definition: BBa_B0015
access: public

BBa_K1065000
type: Component
persistent identity: component2331744
display id: component2331744
version: 1
top level: EFE + T
definition: EFE
access: public

BBa_K1065000
type: SequenceAnnotation
persistent identity: annotation2331744
display id: annotation2331744
version: 1
top level: EFE + T
location: https://synbiohub.org/public/igem/BBa_K1065002/annotation2331744/range2331744/1
component: BBa_K1065000

https://synbiohub.org/public/igem/BBa_K1065002/annotation2331744/range2331744/1
type: Range
persistent identity: range2331744
display id: range2331744
version: 1
top level: EFE + T
direction: direction/reverse
start: 1
end: 1078
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2331758
display id: annotation2331758
version: 1
top level: EFE + T
location: https://synbiohub.org/public/igem/BBa_K1065002/annotation2331758/range2331758/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1065002/annotation2331758/range2331758/1
type: Range
persistent identity: range2331758
display id: range2331758
version: 1
top level: EFE + T
direction: direction/reverse
start: 1087
end: 1215
orientation: inline

BBa_K1065302
type: ComponentDefinition
persistent identity: BBa K1065302
display id: BBa_K1065302
version: 1
was generated by: iGEM to SBOL conversion
description: Blue light sensing device without inverter for the production of amilGFP
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:59Z
mutable provenance: The part come from a BioBrick already present in the registry
owned by: james
owned by: myers
mutable description: In the absence of the blue-light the cell express amilGFP. In the presence of blue-light the cell don't produce amilGFP.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1373_
m user id: 0
owner id: 15990
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: I create this parts insert promoter lacI regulated (BBa_R0010) at the part BBa_K952003 with insertion of RBS (between FixK2 promoter and amilGFP) with mutagenesis
star: false
creator: Bruno Aor
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0010
component: BBa_K1065305
sequence annotation: BBa_R0010
sequence annotation: BBa_K1065305
sequence: https://synbiohub.org/public/igem/BBa_K1065302_sequence/1

BBa_R0010
type: Component
persistent identity: component2368261
display id: component2368261
version: 1
top level: BBa_K1065302
definition: LacI
access: public

BBa_K1065305
type: Component
persistent identity: component2368282
display id: component2368282
version: 1
top level: BBa_K1065302
definition: BBa_K1065305
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2368261
display id: annotation2368261
version: 1
top level: BBa_K1065302
location: https://synbiohub.org/public/igem/BBa_K1065302/annotation2368261/range2368261/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1065302/annotation2368261/range2368261/1
type: Range
persistent identity: range2368261
display id: range2368261
version: 1
top level: BBa_K1065302
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K1065305
type: SequenceAnnotation
persistent identity: annotation2368282
display id: annotation2368282
version: 1
top level: BBa_K1065302
location: https://synbiohub.org/public/igem/BBa_K1065302/annotation2368282/range2368282/1
component: BBa_K1065305

https://synbiohub.org/public/igem/BBa_K1065302/annotation2368282/range2368282/1
type: Range
persistent identity: range2368282
display id: range2368282
version: 1
top level: BBa_K1065302
direction: direction/reverse
start: 209
end: 2982
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

EFE
type: ComponentDefinition
persistent identity: BBa K1065000
display id: BBa_K1065000
version: 1
was generated by: iGEM to SBOL conversion
description: 2-oxoglutarate oxygenase/decarboxylase (EFE)
created: 2013-06-19T11:00:00Z
modified: 2015-05-08T01:08:59Z
mutable provenance: xxxx
owned by: james
owned by: myers
mutable description: xxx
discontinued: false
dominant: false
experience: experience/Works
group u list: _1373_
m user id: 0
owner id: 15969
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: xxx
star: false
creator: Thomas Perli
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: BBa_K1065000
sequence annotation: Spacer
sequence annotation: CDS - Ethylene Forming Enzyme
sequence annotation: Start
sequence annotation: NgoMIV
sequence annotation: AgeI
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K1065000_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2331672
display id: annotation2331672
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331672/range2331672/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331672/range2331672/1
type: Range
persistent identity: range2331672
display id: range2331672
version: 1
top level: EFE
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1065000
type: SequenceAnnotation
persistent identity: annotation2331673
display id: annotation2331673
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331673/range2331673/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331673/range2331673/1
type: Range
persistent identity: range2331673
display id: range2331673
version: 1
top level: EFE
direction: direction/reverse
start: 1
end: 1078
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2331674
display id: annotation2331674
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331674/range2331674/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331674/range2331674/1
type: Range
persistent identity: range2331674
display id: range2331674
version: 1
top level: EFE
direction: direction/forward
start: 7
end: 13
orientation: inline

CDS - Ethylene Forming Enzyme
type: SequenceAnnotation
persistent identity: annotation2331675
display id: annotation2331675
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331675/range2331675/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331675/range2331675/1
type: Range
persistent identity: range2331675
display id: range2331675
version: 1
top level: EFE
direction: direction/forward
start: 14
end: 1078
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2331676
display id: annotation2331676
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331676/range2331676/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331676/range2331676/1
type: Range
persistent identity: range2331676
display id: range2331676
version: 1
top level: EFE
direction: direction/forward
start: 14
end: 16
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2331677
display id: annotation2331677
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331677/range2331677/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331677/range2331677/1
type: Range
persistent identity: range2331677
display id: range2331677
version: 1
top level: EFE
direction: direction/forward
start: 17
end: 22
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2331678
display id: annotation2331678
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331678/range2331678/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331678/range2331678/1
type: Range
persistent identity: range2331678
display id: range2331678
version: 1
top level: EFE
direction: direction/forward
start: 1070
end: 1075
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2331679
display id: annotation2331679
version: 1
top level: EFE
location: https://synbiohub.org/public/igem/BBa_K1065000/annotation2331679/range2331679/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1065000/annotation2331679/range2331679/1
type: Range
persistent identity: range2331679
display id: range2331679
version: 1
top level: EFE
direction: direction/forward
start: 1076
end: 1078
orientation: inline

BBa_K1065305
type: ComponentDefinition
persistent identity: BBa K1065305
display id: BBa_K1065305
version: 1
was generated by: iGEM to SBOL conversion
description: Blue light sensor without inverter for the production of amilGFP
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:59Z
mutable provenance: Igem registry Kit 2013
owned by: james
owned by: myers
mutable description: The part potentially produce amilGFP in the dark (without the blue-light) when assembly with a promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1373_
m user id: 0
owner id: 15990
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Modify the already exist sequence, inserting a RBS between PfixK2 and amilGFP to produce the protein
star: false
creator: Bruno Aor
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0034
sequence annotation: BBa_K592016
sequence annotation: BBa_K1065305
sequence annotation: conserved
sequence annotation: BBa_K592004
sequence annotation: YF1
sequence annotation: FixJ
sequence annotation: BBa_K592005
sequence annotation: BBa_K592006
sequence annotation: FixK2 promoter
sequence annotation: RBS: gaggaggataatct
sequence annotation: BBa_K592010
sequence annotation: amilGFP
sequence: https://synbiohub.org/public/igem/BBa_K1065305_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2368300
display id: annotation2368300
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368300/range2368300/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368300/range2368300/1
type: Range
persistent identity: range2368300
display id: range2368300
version: 1
top level: BBa_K1065305
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K592016
type: SequenceAnnotation
persistent identity: annotation2368301
display id: annotation2368301
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368301/range2368301/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368301/range2368301/1
type: Range
persistent identity: range2368301
display id: range2368301
version: 1
top level: BBa_K1065305
direction: direction/reverse
start: 1
end: 1796
orientation: inline

BBa_K1065305
type: SequenceAnnotation
persistent identity: annotation2368302
display id: annotation2368302
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368302/range2368302/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368302/range2368302/1
type: Range
persistent identity: range2368302
display id: range2368302
version: 1
top level: BBa_K1065305
direction: direction/reverse
start: 1
end: 2774
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2368303
display id: annotation2368303
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368303/range2368303/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368303/range2368303/1
type: Range
persistent identity: range2368303
display id: range2368303
version: 1
top level: BBa_K1065305
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K592004
type: SequenceAnnotation
persistent identity: annotation2368304
display id: annotation2368304
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368304/range2368304/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368304/range2368304/1
type: Range
persistent identity: range2368304
display id: range2368304
version: 1
top level: BBa_K1065305
direction: direction/reverse
start: 19
end: 1149
orientation: inline

YF1
type: SequenceAnnotation
persistent identity: annotation2368305
display id: annotation2368305
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368305/range2368305/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368305/range2368305/1
type: Range
persistent identity: range2368305
display id: range2368305
version: 1
top level: BBa_K1065305
direction: direction/forward
start: 19
end: 1149
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2368306
display id: annotation2368306
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368306/range2368306/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368306/range2368306/1
type: Range
persistent identity: range2368306
display id: range2368306
version: 1
top level: BBa_K1065305
direction: direction/reverse
start: 1158
end: 1169
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2368307
display id: annotation2368307
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368307/range2368307/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368307/range2368307/1
type: Range
persistent identity: range2368307
display id: range2368307
version: 1
top level: BBa_K1065305
direction: direction/forward
start: 1162
end: 1165
orientation: inline

FixJ
type: SequenceAnnotation
persistent identity: annotation2368308
display id: annotation2368308
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368308/range2368308/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368308/range2368308/1
type: Range
persistent identity: range2368308
display id: range2368308
version: 1
top level: BBa_K1065305
direction: direction/forward
start: 1176
end: 1796
orientation: inline

BBa_K592005
type: SequenceAnnotation
persistent identity: annotation2368309
display id: annotation2368309
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368309/range2368309/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368309/range2368309/1
type: Range
persistent identity: range2368309
display id: range2368309
version: 1
top level: BBa_K1065305
direction: direction/reverse
start: 1176
end: 1796
orientation: inline

BBa_K592006
type: SequenceAnnotation
persistent identity: annotation2368311
display id: annotation2368311
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368311/range2368311/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368311/range2368311/1
type: Range
persistent identity: range2368311
display id: range2368311
version: 1
top level: BBa_K1065305
direction: direction/reverse
start: 1805
end: 2054
orientation: inline

FixK2 promoter
type: SequenceAnnotation
persistent identity: annotation2368310
display id: annotation2368310
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368310/range2368310/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368310/range2368310/1
type: Range
persistent identity: range2368310
display id: range2368310
version: 1
top level: BBa_K1065305
direction: direction/forward
start: 1805
end: 2054
orientation: inline

RBS: gaggaggataatct
type: SequenceAnnotation
persistent identity: annotation2368312
display id: annotation2368312
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368312/range2368312/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368312/range2368312/1
type: Range
persistent identity: range2368312
display id: range2368312
version: 1
top level: BBa_K1065305
direction: direction/forward
start: 2061
end: 2075
orientation: inline

BBa_K592010
type: SequenceAnnotation
persistent identity: annotation2368313
display id: annotation2368313
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368313/range2368313/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368313/range2368313/1
type: Range
persistent identity: range2368313
display id: range2368313
version: 1
top level: BBa_K1065305
direction: direction/reverse
start: 2076
end: 2774
orientation: inline

amilGFP
type: SequenceAnnotation
persistent identity: annotation2368314
display id: annotation2368314
version: 1
top level: BBa_K1065305
location: https://synbiohub.org/public/igem/BBa_K1065305/annotation2368314/range2368314/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1065305/annotation2368314/range2368314/1
type: Range
persistent identity: range2368314
display id: range2368314
version: 1
top level: BBa_K1065305
direction: direction/forward
start: 2076
end: 2774
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1065309_sequence/1
type: Sequence
persistent identity: BBa K1065309 sequence
display id: BBa_K1065309_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgatta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1065002_sequence/1
type: Sequence
persistent identity: BBa K1065002 sequence
display id: BBa_K1065002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1065302_sequence/1
type: Sequence
persistent identity: BBa K1065302 sequence
display id: BBa_K1065302_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgatta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1065000_sequence/1
type: Sequence
persistent identity: BBa K1065000 sequence
display id: BBa_K1065000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggaactactatggccggcaccaacctgcaaacatttgaactgccgacggaagtaactggctgcgctgcggacatttcgctgggacgtgccctgatccaagcatggcagaaagatggcatttttcaaatcaaaaccgattcggaacaggaccgtaaaacacaagaagccatggcggcatctaaacagttctgcaaagaaccgctgacgtttaaaagctcatgtgtcagcgatctgacttattcaggatacgtggcgtctggcgaagaagttacggcaggcaaaccggactttccggaaatcttcactgtgtgcaaagatctgtcagtcggtgaccagagagtaaaagctggttggccgtgtcatggcccggtgccgtggccgaacaacacctaccaaaaaagcatgaaaacattcatggaagaactgggcctggccggtgaacgcctgctgaaactgacggctctgggtttcgaactgccgattaatacgtttactgatctgacccgcgacggatggcatcacatgcgtgtgctgagatttccgccgcagaccagtacactgtcgcgtggtattggagcgcataccgattatggactgctggtcatcgccgctcaagatgacgtaggcggtctgtacattcgtccgccggtcgaaggtgaaaaacgcaatcgtaactggctgccgggcgaatcttccgcaggtatgttcgaacacgatgaaccgtggacctttgtaacgccgactccgggagtgtggacagttttcccgggcgacatcctgcaatttatgacgggaggccaactgctgtccactccgcataaagttaaactgaatacaagagaacgctttgcgtgcgcatatttccacgaaccgaactttgaagcctccgcttacccgctgtttgaaccgagtgcaaatgaacgcattcattatggcgaacactttacgaacatgttcatgcgctgttacccggatcgtattaccacacaaagaatcaacaaagaaaatagactggcacacctggaagacctgaaaaaatactcggacacaagagcaactggttcgaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1065305_sequence/1
type: Sequence
persistent identity: BBa K1065305 sequence
display id: BBa_K1065305_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataatactagaggccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z