LOCUS BBa_K1065310 3787 bp DNA linear 22-Nov-2024 DEFINITION Blue light circuit with inverter for the production of amilCP ACCESSION BBa_K1065310 VERSION BBa_K1065310.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..1192 /label=YF1 CDS 62..1192 /label=BBa_K592004 misc_feature 1205..1208 /label=conserved RBS 1201..1212 /label=BBa_B0034 CDS 1219..1839 /label=FixJ CDS 1219..1839 /label=BBa_K592005 misc_feature 44..1839 /label=BBa_K592016 promoter 1848..2097 /label=FixK2 promoter promoter 1848..2097 /label=BBa_K592006 misc_feature 2110..2113 /label=conserved RBS 2106..2117 /label=BBa_B0034 CDS 2127..2834 /label=cI lambda misc_feature 2835..2867 /label=LVA misc_feature 2874..2898 /label=Help:Barcodes CDS 2124..2873 /label=BBa_C0051 stem_loop 2918..2961 /label=stem_loop misc_feature 2907..2986 /label=BBa_B0010 terminator 2907..2986 /label=BBa_B0010 stem_loop 3002..3021 /label=T7 TE misc_feature 3028..3028 /label=stop polyA_site 3022..3035 /label=polya misc_feature 2995..3035 /label=BBa_B0012 terminator 2995..3035 /label=BBa_B0012 promoter 3081..3086 /label=-10 promoter 3058..3063 /label=-35 misc_feature 3068..3084 /label=OR1 misc_feature 3044..3060 /label=OR2 misc_feature 3044..3092 /label=BBa_R0051 promoter 3044..3092 /label=BBa_R0051 misc_feature 2106..3092 /label=BBa_Q04510 misc_feature 3105..3108 /label=conserved RBS 3101..3112 /label=BBa_B0034 CDS 3119..3784 /label=amilCP CDS 3119..3787 /label=BBa_K592009 misc_feature 1848..3787 /label=BBa_K592020 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggctagt tttcaatcat ttgggatacc aggacagctg gaagtcatca aaaaagcact 121 tgatcacgtg cgagtcggtg tggtaattac agatcccgca cttgaagata atcctattgt 181 ctacgtaaat caaggctttg ttcaaatgac cggctacgag accgaggaaa ttttaggaaa 241 gaactgtcgc ttcttacagg ggaaacacac agatccggca gaagtggaca acatcagaac 301 cgctttacaa aataaagaac cggtcaccgt tcagatccaa aactacaaaa aagacggaac 361 gatgttctgg aatgaattaa atattgatcc aatggaaata gaggataaaa cgtattttgt 421 cggcattcag aatgatatca ccgagcacca gcagacccag gcgcgtctcc aggaactgca 481 atccgagctc gtccacgtct ccaggctgag cgccatgggc gaaatggcgt ccgcgctcgc 541 gcacgagctc aaccagccgc tggcggcgat cagcaactac atgaagggct cgcggcggct 601 gcttgccggc agcagtgatc cgaacacacc gaaggtcgaa agcgccctgg accgcgccgc 661 cgagcaggcg ctgcgcgccg gccagatcat ccggcgcctg cgcgacttcg ttgcccgcgg 721 cgaatcggag aagcgggtcg agagtctctc caagctgatc gaggaggccg gcgcgctcgg 781 gcttgccggc gcgcgcgagc agaacgtgca gctccgcttc agtctcgatc cgggcgccga 841 tctcgttctc gccgaccggg tgcagatcca gcaggtcctg gtcaacctgt tccgcaacgc 901 gctggaagcg atggctcagt cgcagcgacg cgagctcgtc gtcaccaaca cccccgccgc 961 cgacgacatg atcgaggtcg aagtgtccga caccggcagc ggtttccagg acgacgtcat 1021 tccgaacctg tttcagactt tcttcaccac caaggacacc ggcatgggcg tgggactgtc 1081 catcagccgc tcgatcatcg aagctcacgg cgggcgcatg tgggccgaga gcaacgcatc 1141 gggcggggcg accttccgct tcaccctccc ggcagccgac gagaattaat aatactagag 1201 aaagaggaga aatactagat gacgaccaag ggacatatct acgtcatcga cgacgacgcg 1261 gcgatgcggg attcgctgaa tttcctgctg gattctgccg gcttcggcgt cacgctgttt 1321 gacgacgcgc aagcctttct cgacgccctg ccgggtctct ccttcggctg cgtcgtctcc 1381 gacgtgcgca tgccgggcct tgacggcatc gagctgttga agcggatgaa ggcgcagcaa 1441 agcccctttc cgatcctcat catgaccggt cacggcgacg tgccgctcgc ggtcgaggcg 1501 atgaagttag gggcggtcga ctttctggaa aagcctttcg aggacgaccg cctcaccgcc 1561 atgatcgaat cggcgatccg ccaggccgag ccggccgcca agagcgaggc cgtcgcgcag 1621 gatatcgccg cccgcgtcgc ctcgttgagc cccagggagc gccaggtcat ggaagggctg 1681 atcgccggcc tttccaacaa gctgatcgcc cgcgagtacg acatcagccc gcgcaccatc 1741 gaggtgtatc gggccaacgt catgaccaag atgcaggcca acagcctttc ggagctggtt 1801 cgcctcgcga tgcgcgccgg catgctcaac gattaataat actagaggcc ggagccgatt 1861 atccgcaccc gtccttggtc ttggacacac tcgctcgcga tccgaagccg cctttcaaag 1921 atactccgca gtgaaacgca tcttttaagc gcgacttgtc gccttcgcgc agcagaacac 1981 tcgtatggca ctcaatccat gatcgcttcg ttcgctccga cgcgcgttgc ggggcccccc 2041 tcgaccggat gacaacacat tgcgtcacct caactgctgc gcgcgcactg cgtcacatac 2101 tagagaaaga ggagaaatac tagatgagca caaaaaagaa accattaaca caagagcagc 2161 ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat 2221 cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt gctttattta 2281 atggcatcaa tgcattaaat gcttataacg ccgcattgct tgcaaaaatt ctcaaagtta 2341 gcgttgaaga atttagccct tcaatcgcca gagaaatcta cgagatgtat gaagcggtta 2401 gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat gttcaggcag 2461 ggatgttctc acctgagctt agaaccttta ccaaaggtga tgcggagaga tgggtaagca 2521 caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat tccatgaccg 2581 caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt gaccctgagc 2641 aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag tttaccttca 2701 agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca cagtacccaa 2761 tgatcccatg caatgagagt tgttccgttg tggggaaagt tatcgctagt cagtggcctg 2821 aagagacgtt tggcgctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat 2881 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2941 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 3001 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtaacacc gtgcgtgttg 3061 actattttac ctctggcggt gataatggtt gctactagag aaagaggaga aatactagat 3121 gagtgtgatc gctaaacaaa tgacctacaa ggtttatatg tcaggcacgg tcaatggaca 3181 ctactttgag gtcgaaggcg atggaaaagg taagccctac gagggggagc agacggtaaa 3241 gctcactgtc accaagggcg gacctctgcc atttgcttgg gatattttat caccacagtg 3301 tcagtacgga agcataccat tcaccaagta ccctgaagac atccctgact atgtaaagca 3361 gtcattcccg gagggctata catgggagag gatcatgaac tttgaagatg gtgcagtgtg 3421 tactgtcagc aatgattcca gcatccaagg caactgtttc atctaccatg tcaagttctc 3481 tggtttgaac tttcctccca atggacctgt catgcagaag aagacacagg gctgggaacc 3541 caacactgag cgtctctttg cacgagatgg aatgctgcta ggaaacaact ttatggctct 3601 gaagttagaa ggaggcggtc actatttgtg tgaatttaaa actacttaca aggcaaagaa 3661 gcctgtgaag atgccagggt atcactatgt tgaccgcaaa ctggatgtaa ccaatcacaa 3721 caaggattac acttcggttg agcagtgtga aatttccatt gcacgcaaac ctgtggtcgc 3781 ctaataa //