LOCUS BBa_K106672 6638 bp DNA linear 01-Jun-2024 DEFINITION AarI AD acceptor vector (pRS305, Gal1P, Adh1t) ACCESSION BBa_K106672 VERSION BBa_K106672.1 FEATURES Location/Qualifiers CDS 1570..2676 /label=LEU2 marker CDS 3539..4399 /label=ampicillin resistance marker promoter 5618..6634 /label=Gal1 Promoter stem_loop 30..215 /label=Adh1 terminator misc_feature 1..4 /label=D overhang promoter 224..242 /label=T7 promoter misc_feature 246..405 /label=lacZalpha reporter misc_feature 427..733 /label=f1 origin promoter 3469..3497 /label=ampicillin resistance promoter misc_feature 4554..5173 /label=pBR322 origin promoter 5482..5511 /label=lac promoter promoter 5581..5600 /label=T3 promoter promoter 6172..6621 /label=Gal1 promoter misc_feature 11..21 /label=Stop codons in all 3 frames misc_feature 6626..6628 /label=Start codon misc_feature 6635..6638 /label=A overhang ORIGIN 1 tgcgggatcc tgagtaaata agcggccgcg cgaatttctt atgatttatg atttttatta 61 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa 121 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat 181 agcatgaggt cgctcttatt gaccacaccg agctccaatt cgccctatag tgagtcgtat 241 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 301 cttaatcgcc ttgcagcaca tccccccttc gccagctggc gtaatagcga agaggcccgc 361 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt 421 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 481 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 541 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 601 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 661 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 721 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 781 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 841 aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg 901 gtatttcaca ccgcatatcg accctcgagg agaacttcta gtatatccac atacctaata 961 ttattgcctt attaaaaatg gaatcccaac aattacatca aaatccacat tctcttcaaa 1021 atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt tataggataa 1081 ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa ggcgcctgat 1141 tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta agatgcaaga 1201 gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca cacaggggcg 1261 ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag aggtcgcctg 1321 acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag aggccggaac 1381 cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga 1441 agttgacaat attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt 1501 actttctaac ttttcttacc ttttacattt cagcaatata tatatatatt tcaaggatat 1561 accattctaa tgtctgcccc tatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac 1621 cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt 1681 cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc tatcgatgct 1741 acaggtgtcc cacttccaga tgaggcgctg gaagcctcca agaaggttga tgccgttttg 1801 ttaggtgctg tgggtggtcc taaatggggt accggtagtg ttagacctga acaaggttta 1861 ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc 1921 gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt 1981 gttgtcagag aattagtggg aggtatttac tttggtaaga gaaaggaaga cgatggtgat 2041 ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat cacaagaatg 2101 gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt ggataaagct 2161 aatgttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat caagaacgaa 2221 ttccctacat tgaaggttca acatcaattg attgattctg ccgccatgat cctagttaag 2281 aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga tatcatctcc 2341 gatgaagcct ccgttatccc aggttccttg ggtttgttgc catctgcgtc cttggcctct 2401 ttgccagaca agaacaccgc atttggtttg tacgaaccat gccacggttc tgctccagat 2461 ttgccaaaga ataaggttga ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa 2521 ttgtcattga acttgcctga agaaggtaag gccattgaag atgcagttaa aaaggttttg 2581 gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga agtcggtgat 2641 gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttatgatat 2701 ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga 2761 atatgttcat agggtagacg aaactatata cgcaatctac atacatttat caagaaggag 2821 aaaaaggagg atagtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat 2881 aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa 2941 gttaggtgta acagaaaatc atgaaactac gattcctaat ttgatattgg aggattttct 3001 ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt 3061 ttaagtcaat accttcttga accatttccc ataatggtga aagttccctc aagaatttta 3121 ctctgtcaga aacggcctta cgacgtagtc gatatggtgc actctcagta caatctgctc 3181 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg 3241 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 3301 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg 3361 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 3421 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 3481 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 3541 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 3601 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 3661 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 3721 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 3781 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 3841 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 3901 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 3961 aggaccgaag gagctaaccg ctttttttca caacatgggg gatcatgtaa ctcgccttga 4021 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 4081 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 4141 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 4201 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 4261 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 4321 gacgggcagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 4381 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 4441 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 4501 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 4561 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 4621 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 4681 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 4741 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 4801 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 4861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 4921 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct 4981 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 5041 cacgagggag cttccagggg ggaacgcctg gtatctttat agtcctgtcg ggtttcgcca 5101 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggccgagcc tatggaaaaa 5161 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 5221 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 5281 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 5341 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 5401 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttacct 5461 cactcattag gcaccccagg ctttacactt tatgcttccg gctcctatgt tgtgtggaat 5521 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcgg 5581 aattaaccct cactaaaggg aacaaaagct gggtaccggg ccctgttatt gtgatagtat 5641 ctcagcggga tttgtgtaga ttcacctaca gcctttaaac cagcaaagtg aattaccgaa 5701 tcaattttat attctttgaa aaccttttcc agaccttttc ggtcacacaa atcaacctca 5761 tagaagggaa tgtgatgctt ggtcaagacc tctaacctgg ctacagaatc ataagttgaa 5821 ttcgacaggt tatcagcaac aacacagtca tatccattct caattagctc taccacagtg 5881 tgtgaaccaa tgtatccagc accacctgta accaaaacaa ttttagaagt actttcactt 5941 tgtaactgag ctgtcattta tattgaattt tcaaaaattc ttactttttt tttggatgga 6001 cgcaaagaag tttaataatc atattacatg gcattaccac catatacata tccatatcta 6061 atcttactta tatgttgtgg aaatgtaaag agccccatta tcttagccta aaaaaacctt 6121 ctctttggaa ctttcagtaa tacgcttaac tgctcattgc tatattgaag tacggattag 6181 aagccgccga gcgggcgaca gccctccgac ggaagactct cctccgtgcg tcctcgtctt 6241 caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg aacaataaag 6301 attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa cctggcccca 6361 caaaccttca aattaacgaa tcaaattaac aaccatagga tgataatgcg attagttttt 6421 tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta ttaacagata 6481 tataaatgga aaagctgcat aaccacttta actaatactt tcaacatttt cagtttgtat 6541 tacttcttat tcaaatgtca taaaagtatc aacaaaaaat tgttaatata cctctatact 6601 ttaacgtcaa ggagaaaaaa ctataatgct cgagggag //