BBa_K106692 1 BBa_K106692 Sir3, AarI A!D part (A!D variant!) 2008-10-28T12:00:00Z 2015-05-08T01:08:59Z Sir3 was PCR amplified from yeast genomic DNA. Sir3 is the silencing protein that recognizes de-acetylated histone tails, and in combination with Sir4, recruits more Sir2 to propagate spreading of silencing. false false _226_ 0 2659 9 It's complicated true Sir3 does not tolerate amino-terminal fusions, so we have redesigned the AarI A site. The new site is termed A!, introduces no additional amino acids at the N-terminus, and only works for cloning into an A! acceptor vector. For more information, please see the UCSF 2008 iGEM wiki. true Andrew Horwitz BBa_K106692_sequence 1 atggctaaaacattgaaagatttggacggttggcaagttatcattacagatgatcaggggagggttattgacgacaacaatagaagaagatcaagaaaaagaggaggagaaaacgtatttctgaaaagaatttctgatggattgagttttgggaagggtgagagcgtgatatttaatgataatgtgacggaaacatattctgtctacttgatccatgagatcagactcaatacacttaataatgttgtggagatttgggttttttcttacttgagatggtttgaactcaaaccaaaactgtactacgaacaattcaggccagatctaataaaggaagatcatcctttagaattttacaaagataaatttttcaacgaagtaaataagagcgagttatatttaactgctgagctatcagagatttggcttaaggatttcattgcagttggacagatactgccagagtcacaatggaatgatagtagtatcgataaaatagaggatagagattttctagtacgttatgcatgcgaacctactgcggaaaagtttgtaccaattgatatttttcaaatcataagaagagtaaaggaaatggaacccaaacaatctgatgaatacttgaaaagagtatctgtaccagtgagtgggcagaagacaaatagacaggtgatgcataagatgggagttgaaagatcttcaaaaagactagcaaagaaaccttcaatgaaaaaaattaaaatcgagccctctgcagatgatgacgtaaataatggaaatataccgtctcagcgaggaacgtcaacaactcatggttcaatttctccccaagaagagtctgtttcccctaacatctcatcggcgtccccttctgcgctcacatcacctacagattcttcgaaaattttacaaaaaagatctatatcaaaggaactaattgtttcagaggaaataccaataaactcttcagaacaggaatccgattatgagccaaacaatgaaacatctgtgctttcaagtaaacctgggtcaaagccagagaagacatctacagaactcgttgacggccgagagaattttgtatatgcaaataatccagaagtgagtgatgatggtgggcttgaagaggaaacagatgaagttagttcagaaagttctgatgaagcaataataccagtaaataaaagacgtggcgctcacggaagcgaattgagcagtaaaattagaaaaatccacattcaagaaacacaagaattttctaaaaattacactacagaaacagataacgaaatgaacggaaatggaaaacctggaattcccaggggcaacaccaaaattcatagtatgaacgaaaatcctacaccagaaaaaggtaatgcaaagatgattgatttcgctactttgtcaaagttgaaaaaaaaatatcagataattttagaccggtttgcaccagataatcaagtaacagattcctcccaattgaacaaattaacagacgaacagtcaagcttggacgttgcaggccttgaggataagtttagaaaagcatgctcttcatctggaagagaaaccattctgtcaaattttaacgctgatataaatttagaggaatcaattagagaatctttacaaaagagagaactactaaaatcacaagtggaggattttacaagaatattccttccgatttacgacagtttgatgtcttcccaaaataaactattttatattaccaatgcagatgattctactaaattccagcttgtgaatgatgtaatggatgagttgatcacctcttcggcccgaaaggaactacctattttcgattacattcatattgatgccttggaactggcaggaatggatgcattatatgagaaaatatggttcgccatttctaaagaaaatctatgcggtgatatatcactagaggccttaaacttctatattacaaatgtcccgaaagcaaaaaagagaaagacactaatattaatacaaaatccggaaaatctattgagtgagaagattttacaatatttcgagaaatggatttcttcgaaaaattcaaaattgtctatcatttgcgttggcggacataatgtgacgattagagagcaaataaatataatgccctccctcaaagcacactttaccgaaatcaaacttaataaggtggacaagaatgaattgcaacaaatgatcatcacgcgactcaaatctttattgaaaccttttcatgtcaaagtaaatgataagaaggaaatgactatttacaataatattcgcgaaggacagaaccagaaaataccagataatgtgatagtaatcaatcataagatcaacaacaaaatcactcaacttattgcaaagaacgtagccaacgtaagtggtagtactgaaaaagcattcaaaatatgtgaagctgccgtagaaatttctaaaaaagacttcgtacggaaaggtggtctacaaaagggtaaattggtagtgtcacaggagatggtaccacgctatttctcagaagctattaacggattcaaggatgaaactatttcaaagaagattataggaatgtctctgttgatgagaacatttttatataccctggcgcaagaaaccgaaggcacgaatcgtcatacgcttgctctggagaccgtcctgattaagatggtgaagatgttgcgggacaacccagggtacaaggcgtcaaaagaaattaagaaggtcatatgcggtgcctgggagcccgcaataactatagagaaactaaagcaattttcttggataagtgtagtgaatgatttagtgggagaaaaattagttgttgtggtgctcgaggagcccagtgcaagcattatggtagaactaaaacttcctttagaaataaattacgccttttcgatggatgaagaattcaaaaatatggactgcatttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z