LOCUS BBa_K106695 4657 bp DNA linear 22-Dec-2024 DEFINITION Sir3 under a strong constitutive promoter ACCESSION BBa_K106695 VERSION BBa_K106695.1 FEATURES Location/Qualifiers promoter 1..1511 /label=BBa_K106694 CDS 1512..4448 /label=BBa_K106692 misc_feature 4449..4477 /label=BBa_K106017 terminator 4478..4657 /label=BBa_K106018 ORIGIN 1 gggccctaaa acaagaagag ggttgactac atcacgatga gggggatcga agaaatgatg 61 gtaaatgaaa taggaaatca aggagcatga aggcaaaaga caaatataag ggtcgaacga 121 aaaataaagt gaaaagtgtt gatatgatgt atttggcttt gcggcgccga aaaaacgagt 181 ttacgcaatt gcacaatcat gctgactctg tggcggaccc gcgctcttgc cggcccggcg 241 ataacgctgg gcgtgaggct gtgcccggcg gagttttttg cgcctgcatt ttccaaggtt 301 taccctgcgc taaggggcga gattggagaa gcaataagaa tgccggttgg ggttgcgatg 361 atgacgacca cgacaactgg tgtcattatt taagttgccg aaagaacctg agtgcatttg 421 caacatgagt atactagaag aatgagccaa gacttgcgag acgcgagttt gccggtggtg 481 cgaacaatag agcgaccatg accttgaagg tgagacgcgc ataaccgcta gagtactttg 541 aagaggaaac agcaataggg ttgctaccag tataaataga caggtacata caacactgga 601 aatggttgtc tgtttgagta cgctttcaat tcatttgggt gtgcacttta ttatgttaca 661 atatggaagg gaactttaca cttctcctat gcacatatat taattaaagt ccaatgctag 721 tagagaaggg gggtaacacc cctccgcgct cttttccgat ttttttctaa accgtggaat 781 atttcggata tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa 841 ccaaacccat acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg 901 cggaggggag atatacaata gaacagatac cagacaagac ataatgggct aaacaagact 961 acaccaatta cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga 1021 cttgatagcc atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa 1081 gtaataatag gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt 1141 gtctcaccat atccgcaatg acaaaaaaat gatggaagac actaaaggaa aaaattaacg 1201 acaaagacag caccaacaga tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac 1261 acgaaacttt ttccttcctt cattcacgca cactactctc taatgagcaa cggtatacgg 1321 ccttccttcc agttacttga atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta 1381 taaatagacc tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt 1441 ccttgtttct ttttctgcac aatatttcaa gctataccaa gcatacaatc aactatctca 1501 tatacaaagc aatggctaaa acattgaaag atttggacgg ttggcaagtt atcattacag 1561 atgatcaggg gagggttatt gacgacaaca atagaagaag atcaagaaaa agaggaggag 1621 aaaacgtatt tctgaaaaga atttctgatg gattgagttt tgggaagggt gagagcgtga 1681 tatttaatga taatgtgacg gaaacatatt ctgtctactt gatccatgag atcagactca 1741 atacacttaa taatgttgtg gagatttggg ttttttctta cttgagatgg tttgaactca 1801 aaccaaaact gtactacgaa caattcaggc cagatctaat aaaggaagat catcctttag 1861 aattttacaa agataaattt ttcaacgaag taaataagag cgagttatat ttaactgctg 1921 agctatcaga gatttggctt aaggatttca ttgcagttgg acagatactg ccagagtcac 1981 aatggaatga tagtagtatc gataaaatag aggatagaga ttttctagta cgttatgcat 2041 gcgaacctac tgcggaaaag tttgtaccaa ttgatatttt tcaaatcata agaagagtaa 2101 aggaaatgga acccaaacaa tctgatgaat acttgaaaag agtatctgta ccagtgagtg 2161 ggcagaagac aaatagacag gtgatgcata agatgggagt tgaaagatct tcaaaaagac 2221 tagcaaagaa accttcaatg aaaaaaatta aaatcgagcc ctctgcagat gatgacgtaa 2281 ataatggaaa tataccgtct cagcgaggaa cgtcaacaac tcatggttca atttctcccc 2341 aagaagagtc tgtttcccct aacatctcat cggcgtcccc ttctgcgctc acatcaccta 2401 cagattcttc gaaaatttta caaaaaagat ctatatcaaa ggaactaatt gtttcagagg 2461 aaataccaat aaactcttca gaacaggaat ccgattatga gccaaacaat gaaacatctg 2521 tgctttcaag taaacctggg tcaaagccag agaagacatc tacagaactc gttgacggcc 2581 gagagaattt tgtatatgca aataatccag aagtgagtga tgatggtggg cttgaagagg 2641 aaacagatga agttagttca gaaagttctg atgaagcaat aataccagta aataaaagac 2701 gtggcgctca cggaagcgaa ttgagcagta aaattagaaa aatccacatt caagaaacac 2761 aagaattttc taaaaattac actacagaaa cagataacga aatgaacgga aatggaaaac 2821 ctggaattcc caggggcaac accaaaattc atagtatgaa cgaaaatcct acaccagaaa 2881 aaggtaatgc aaagatgatt gatttcgcta ctttgtcaaa gttgaaaaaa aaatatcaga 2941 taattttaga ccggtttgca ccagataatc aagtaacaga ttcctcccaa ttgaacaaat 3001 taacagacga acagtcaagc ttggacgttg caggccttga ggataagttt agaaaagcat 3061 gctcttcatc tggaagagaa accattctgt caaattttaa cgctgatata aatttagagg 3121 aatcaattag agaatcttta caaaagagag aactactaaa atcacaagtg gaggatttta 3181 caagaatatt ccttccgatt tacgacagtt tgatgtcttc ccaaaataaa ctattttata 3241 ttaccaatgc agatgattct actaaattcc agcttgtgaa tgatgtaatg gatgagttga 3301 tcacctcttc ggcccgaaag gaactaccta ttttcgatta cattcatatt gatgccttgg 3361 aactggcagg aatggatgca ttatatgaga aaatatggtt cgccatttct aaagaaaatc 3421 tatgcggtga tatatcacta gaggccttaa acttctatat tacaaatgtc ccgaaagcaa 3481 aaaagagaaa gacactaata ttaatacaaa atccggaaaa tctattgagt gagaagattt 3541 tacaatattt cgagaaatgg atttcttcga aaaattcaaa attgtctatc atttgcgttg 3601 gcggacataa tgtgacgatt agagagcaaa taaatataat gccctccctc aaagcacact 3661 ttaccgaaat caaacttaat aaggtggaca agaatgaatt gcaacaaatg atcatcacgc 3721 gactcaaatc tttattgaaa ccttttcatg tcaaagtaaa tgataagaag gaaatgacta 3781 tttacaataa tattcgcgaa ggacagaacc agaaaatacc agataatgtg atagtaatca 3841 atcataagat caacaacaaa atcactcaac ttattgcaaa gaacgtagcc aacgtaagtg 3901 gtagtactga aaaagcattc aaaatatgtg aagctgccgt agaaatttct aaaaaagact 3961 tcgtacggaa aggtggtcta caaaagggta aattggtagt gtcacaggag atggtaccac 4021 gctatttctc agaagctatt aacggattca aggatgaaac tatttcaaag aagattatag 4081 gaatgtctct gttgatgaga acatttttat ataccctggc gcaagaaacc gaaggcacga 4141 atcgtcatac gcttgctctg gagaccgtcc tgattaagat ggtgaagatg ttgcgggaca 4201 acccagggta caaggcgtca aaagaaatta agaaggtcat atgcggtgcc tgggagcccg 4261 caataactat agagaaacta aagcaatttt cttggataag tgtagtgaat gatttagtgg 4321 gagaaaaatt agttgttgtg gtgctcgagg agcccagtgc aagcattatg gtagaactaa 4381 aacttccttt agaaataaat tacgcctttt cgatggatga agaattcaaa aatatggact 4441 gcatttgatg cgggatcctg agtaaataag cggccgcgcg aatttcttat gatttatgat 4501 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta 4561 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc 4621 tcaggtatag catgaggtcg ctcttattga ccacacc //