LOCUS BBa_K106697 6559 bp DNA linear 22-Nov-2024 DEFINITION AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops) ACCESSION BBa_K106697 VERSION BBa_K106697.1 FEATURES Location/Qualifiers misc_feature 3565..4083 /label=CEN ARS CDS 4215..5075 /label=ampicillin resistance marker promoter 6294..6556 /label=Cyc1 Promoter misc_feature 1..4 /label=D overhang stem_loop 30..209 /label=Adh1 terminator misc_feature 210..377 /label=8X LexA Operators CDS 1732..2838 /label=LEU2 marker promoter 386..404 /label=T7 promoter misc_feature 408..567 /label=lacZalpha reporter misc_feature 589..895 /label=f1 origin promoter 4145..4173 /label=ampicillin resistance promoter misc_feature 5230..5849 /label=pBR322 origin promoter 6158..6187 /label=lac promoter promoter 6257..6276 /label=T3 promoter misc_feature 11..21 /label=Stop codons in all 3 frames misc_feature 6547..6549 /label=Start codon misc_feature 6556..6559 /label=A overhang ORIGIN 1 tgcgggatcc tgagtaaata agcggccgcg cgaatttctt atgatttatg atttttatta 61 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa 121 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat 181 agcatgaggt cgctcttatt gaccacaccg agctttcgac tgctgtatat aaaaccagtg 241 gttatatgta cagtactgct gtatataaaa ccagtggtta tatgtacagt acgtcgactg 301 ctgtatataa aaccagtggt tatatgtaca gtactgctgt atataaaacc agtggttata 361 tgtacagtac ggagctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc 421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 481 catcccccct tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 541 cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg 601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 721 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 781 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 901 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 961 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt 1021 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat 1081 cgaccctcga ggagaacttc tagtatatcc acatacctaa tattattgcc ttattaaaaa 1141 tggaatccca acaattacat caaaatccac attctcttca aaatcaattg tcctgtactt 1201 ccttgttcat gtgtgttcaa aaacgttata tttataggat aattatactc tatttctcaa 1261 caagtaattg gttgtttggc cgagcggtct aaggcgcctg attcaagaaa tatcttgacc 1321 gcagttaact gtgggaatac tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa 1381 ccattatttt tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa 1441 ttcgatgact ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca 1501 actgaaaaat tgggagaaaa aggaaaggtg agaggccgga accggctttt catatagaat 1561 agagaagcgt tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta 1621 aggacctatt gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta 1681 ccttttacat ttcagcaata tatatatata tttcaaggat ataccattct aatgtctgcc 1741 cctatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc 1801 acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa tgtcaagttc 1861 gatttcgaaa atcatttaat tggtggtgct gctatcgatg ctacaggtgt cccacttcca 1921 gatgaggcgc tggaagcctc caagaaggtt gatgccgttt tgttaggtgc tgtgggtggt 1981 cctaaatggg gtaccggtag tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa 2041 cttcaattgt acgccaactt aagaccatgt aactttgcat ccgactctct tttagactta 2101 tctccaatca agccacaatt tgctaaaggt actgacttcg ttgttgtcag agaattagtg 2161 ggaggtattt actttggtaa gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt 2221 gaacaataca ccgttccaga agtgcaaaga atcacaagaa tggccgcttt catggcccta 2281 caacatgagc caccattgcc tatttggtcc ttggataaag ctaatgtttt ggcctcttca 2341 agattatgga gaaaaactgt ggaggaaacc atcaagaacg aattccctac attgaaggtt 2401 caacatcaat tgattgattc tgccgccatg atcctagtta agaacccaac ccacctaaat 2461 ggtattataa tcaccagcaa catgtttggt gatatcatct ccgatgaagc ctccgttatc 2521 ccaggttcct tgggtttgtt gccatctgcg tccttggcct ctttgccaga caagaacacc 2581 gcatttggtt tgtacgaacc atgccacggt tctgctccag atttgccaaa gaataaggtt 2641 gaccctatcg ccactatctt gtctgctgca atgatgttga aattgtcatt gaacttgcct 2701 gaagaaggta aggccattga agatgcagtt aaaaaggttt tggatgcagg tatcagaact 2761 ggtgatttag gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt 2821 aagaaaatcc ttgcttaaaa agattctctt tttttatgat atttgtacat aaactttata 2881 aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga 2941 cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga ggatagtaaa 3001 ggaatacagg taagcaaatt gatactaatg gctcaacgtg ataaggaaaa agaattgcac 3061 tttaacatta atattgacaa ggaggagggc accacacaaa aagttaggtg taacagaaaa 3121 tcatgaaact acgattccta atttgatatt ggaggatttt ctctaaaaaa aaaaaaatac 3181 aacaaataaa aaacactcaa tgacctgacc atttgatgga gtttaagtca ataccttctt 3241 gaaccatttc ccataatggt gaaagttccc tcaagaattt tactctgtca gaaacggcct 3301 tacgacgtag tcgatatggt gcactctcag tacaatctgc tctgatgccg catagttaag 3361 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 3421 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 3481 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa 3541 tgtcatgata ataatggttt cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg 3601 tatcttttaa tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt 3661 gtatttggat tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa 3721 aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga 3781 caagaaaagc agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac 3841 aggattttcg tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg 3901 agagcaggaa gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac 3961 atcttcggaa aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat 4021 ttatatattt atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg 4081 acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 4141 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 4201 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 4261 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 4321 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 4381 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 4441 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 4501 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 4561 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 4621 ttctgacaac gatcggagga ccgaaggagc taaccgcttt ttttcacaac atgggggatc 4681 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 4741 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 4801 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 4861 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 4921 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 4981 tcgtagttat ctacacgacg ggcagtcagg caactatgga tgaacgaaat agacagatcg 5041 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 5101 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 5161 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 5221 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 5281 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 5341 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 5401 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 5461 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 5521 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 5581 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt 5641 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 5701 tcggaacagg agagcgcacg agggagcttc caggggggaa cgcctggtat ctttatagtc 5761 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 5821 cgagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 5881 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 5941 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 6001 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 6061 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 6121 ttaatgtgag ttacctcact cattaggcac cccaggcttt acactttatg cttccggctc 6181 ctatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 6241 attacgccaa gctcggaatt aaccctcact aaagggaaca aaagctgggt accgggcccg 6301 agcagatccg ccaggcgtgt atatatagcg tggatggcca ggcaacttta gtgctgacac 6361 atacaggcat atatatatgt gtgcgacgac acatgatcat atggcatgca tgtgctctgt 6421 atgtatataa aactcttgtt ttcttctttt ctctaaatat tctttcctta tacattagga 6481 cctttgcagc ataaattact atacttctat agacacacaa acacaaatac acacactaaa 6541 ttaataatgc tcgagggag //