LOCUS BBa_K106698 7364 bp DNA linear 25-Nov-2024 DEFINITION AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t) ACCESSION BBa_K106698 VERSION BBa_K106698.1 FEATURES Location/Qualifiers misc_feature 3403..3921 /label=CEN ARS CDS 4053..4913 /label=ampicillin resistance marker misc_feature 6132..6299 /label=8X LexA Operators promoter 6300..7309 /label=Fig1 Promoter stem_loop 22..215 /label=Adh1 terminator CDS 1570..2676 /label=LEU2 marker promoter 224..242 /label=T7 promoter misc_feature 246..405 /label=lacZalpha reporter misc_feature 427..733 /label=f1 origin promoter 2983..4011 /label=ampicillin marker promoter misc_feature 5068..5687 /label=pBR322 origin promoter 5996..6025 /label=lac promoter promoter 6095..6114 /label=T3 promoter misc_feature 1..4 /label=D overhang misc_feature 11..21 /label=Stop codons in all 3 frames misc_feature 7309..7312 /label=A overhang ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata 121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt 181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa 241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata 301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct 361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata 421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact 481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa 541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg 601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1141 ccgaaggagc taaccgcttt ttttcacaac atgggggatc atgtaactcg ccttgatcgt 1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 1501 ggcagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2101 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc 2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2221 agggagcttc caggggggaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc cgagcctatg gaaaaacgcc 2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact 2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg 2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcggaatt 2761 aaccctcact aaagggaaca aaagctgggt accgggcccc gtactgtaca tataaccact 2821 ggttttatat acagcagtac tgtacatata accactggtt ttatatacag cagtcgacgt 2881 actgtacata taaccactgg ttttatatac agcagtactg tacatataac cactggtttt 2941 atatacagca gtcgagggcc tttgaatgat caaccaaacg ccgatatagt cactattggt 3001 gataaaatta aaatgcagct attgagaacc catcctaaca acattaaatt gtctattaat 3061 ggaattggta aagatgcccc aactttccaa gaatctgctt tgattgccga taagttattg 3121 agtgtcatga aggccggcac ttacccaaag atttccattt tctacaatga cccagtgtct 3181 tccctatctt ttgaaccatc tgaaaaaccg atctttaacg ccaagaccat tgaacaatcc 3241 ccatcattcg gcaaatttga gatcgacacg gacgcaaacg ttccaagaga tttgtttgaa 3301 tatactttgg ctaaccaaat gttgacagca atggctcaag gttatgctgc tgaaatttcc 3361 gccagaagaa acgctatgga taacgcttcc aagaatgccg gtgatatgat caatcgttac 3421 tctatcttgt acaacagaac aagacaagct gtcattacta atgaactggt tgatattatt 3481 actggtgctt cctctttggg atgataaaaa aatcaccctg cattgcctct ttatttgacg 3541 ttgtttttgt agaacatgaa acgaattttg acttgatgag acgaagtata tatccaaaga 3601 ataccttaaa tagaaaagga aagataataa atactaaaca ctactatata ttcaggtaaa 3661 atacaaaaat tataacattt ttaaaacttt ttttttttga aagtccttct cgctttagga 3721 ttttttccca ttaagattat gatggtttca tgtatgtgtc agttaaaaaa aatatggcta 3781 agtagcaatg aaacgaacca agaaaatgaa aacaatatat agtgctgttg aaataacaaa 3841 gacattggta tatatttgta aaatgtctgt taaatgtttt ttatctcagg ttcttgcttg 3901 tctttggtag aagaaattat agtaaacaaa caaacaaaca aacaaaaaaa aaaaaaaaaa 3961 aatgctcgag ggagcaaggc aggtggacaa gaggagtccc gggagctgga actcccacct 4021 gcaacatgcg ggatcctgag taaataagcg gccgcgcgaa tttcttatga tttatgattt 4081 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg 4141 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc 4201 aggtatagca tgaggtcgct cttattgacc acaccgagct ccaattcgcc ctatagtgag 4261 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 4321 acccaactta atcgccttgc agcacatccc cccttcgcca gctggcgtaa tagcgaagag 4381 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg 4441 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 4501 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 4561 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 4621 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 4681 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 4741 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 4801 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 4861 aattttaaca aaatattaac gtttacaatt tcctgatgcg gtattttctc cttacgcatc 4921 tgtgcggtat ttcacaccgc atatcgaccc tcgaggagaa cttctagtat atccacatac 4981 ctaatattat tgccttatta aaaatggaat cccaacaatt acatcaaaat ccacattctc 5041 ttcaaaatca attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt tatatttata 5101 ggataattat actctatttc tcaacaagta attggttgtt tggccgagcg gtctaaggcg 5161 cctgattcaa gaaatatctt gaccgcagtt aactgtggga atactcaggt atcgtaagat 5221 gcaagagttc gaatctctta gcaaccatta tttttttcct caacataacg agaacacaca 5281 ggggcgctat cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt 5341 cgcctgacgc atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagaggc 5401 cggaaccggc ttttcatata gaatagagaa gcgttcatga ctaaatgctt gcatcacaat 5461 acttgaagtt gacaatatta tttaaggacc tattgttttt tccaataggt ggttagcaat 5521 cgtcttactt tctaactttt cttacctttt acatttcagc aatatatata tatatttcaa 5581 ggatatacca ttctaatgtc tgcccctatg tctgccccta agaagatcgt cgttttgcca 5641 ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa agctatttct 5701 gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg tgctgctatc 5761 gatgctacag gtgtcccact tccagatgag gcgctggaag cctccaagaa ggttgatgcc 5821 gttttgttag gtgctgtggg tggtcctaaa tggggtaccg gtagtgttag acctgaacaa 5881 ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc atgtaacttt 5941 gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa aggtactgac 6001 ttcgttgttg tcagagaatt agtgggaggt atttactttg gtaagagaaa ggaagacgat 6061 ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca aagaatcaca 6121 agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg gtccttggat 6181 aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga aaccatcaag 6241 aacgaattcc ctacattgaa ggttcaacat caattgattg attctgccgc catgatccta 6301 gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt tggtgatatc 6361 atctccgatg aagcctccgt tatcccaggt tccttgggtt tgttgccatc tgcgtccttg 6421 gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca cggttctgct 6481 ccagatttgc caaagaataa ggttgaccct atcgccacta tcttgtctgc tgcaatgatg 6541 ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc agttaaaaag 6601 gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac caccgaagtc 6661 ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc tcttttttta 6721 tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac gaaattacaa 6781 aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag 6841 aaggagaaaa aggaggatag taaaggaata caggtaagca aattgatact aatggctcaa 6901 cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga gggcaccaca 6961 caaaaagtta ggtgtaacag aaaatcatga aactacgatt cctaatttga tattggagga 7021 ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga 7081 tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt tccctcaaga 7141 attttactct gtcagaaacg gccttacgac gtagtcgata tggtgcactc tcagtacaat 7201 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc 7261 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 7321 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcga //