LOCUS       BBa_K1067001            1584 bp    DNA     linear       25-Nov-2024
DEFINITION  Twin-arginine translocation reporter
ACCESSION   BBa_K1067001
VERSION     BBa_K1067001.1  
FEATURES             Location/Qualifiers
     misc_feature    23..139
                     /label=TAT signal peptide
     CDS             140..853
                     /label=GFP SF
     misc_feature    854..873
                     /label=Gap
     RBS             862..866
                     /label=rbs
     misc_feature    23..25
                     /label=start
     misc_feature    851..853
                     /label=stop
     misc_feature    874..876
                     /label=start
     misc_feature    1582..1584
                     /label=stop
     CDS             874..1584
                     /label=RFP mCherry
ORIGIN
        1 ttcattaaag aggagaaagg taatgaataa taacgacctg tttcaggcgt cgcgtcgacg
       61 attcctcgca cagctaggtg ggttgacagt ggcgggaatg ttagggccgt cgctcctaac
      121 tcctcgacgg gctacagccc gtaaaggcga agagctgttc actggtgtcg tccctattct
      181 ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg
      241 tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt
      301 accttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc
      361 ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga
      421 acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga
      481 aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa
      541 tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga
      601 taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag
      661 cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct
      721 gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg
      781 cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga
      841 actgtacaaa tgacattaaa gaggagaaag gtaatggtga gcaagggcga ggaggataac
      901 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      961 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     1021 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     1081 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     1141 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     1201 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     1261 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     1321 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     1381 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     1441 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     1501 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     1561 ggcatggacg agctgtacaa gtaa
//