LOCUS BBa_K1067001 1584 bp DNA linear 05-Nov-2024 DEFINITION Twin-arginine translocation reporter ACCESSION BBa_K1067001 VERSION BBa_K1067001.1 FEATURES Location/Qualifiers misc_feature 23..139 /label=TAT signal peptide CDS 140..853 /label=GFP SF misc_feature 854..873 /label=Gap RBS 862..866 /label=rbs misc_feature 23..25 /label=start misc_feature 851..853 /label=stop misc_feature 874..876 /label=start misc_feature 1582..1584 /label=stop CDS 874..1584 /label=RFP mCherry ORIGIN 1 ttcattaaag aggagaaagg taatgaataa taacgacctg tttcaggcgt cgcgtcgacg 61 attcctcgca cagctaggtg ggttgacagt ggcgggaatg ttagggccgt cgctcctaac 121 tcctcgacgg gctacagccc gtaaaggcga agagctgttc actggtgtcg tccctattct 181 ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg 241 tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt 301 accttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc 361 ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga 421 acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga 481 aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa 541 tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga 601 taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag 661 cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct 721 gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg 781 cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga 841 actgtacaaa tgacattaaa gaggagaaag gtaatggtga gcaagggcga ggaggataac 901 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 961 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 1021 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 1081 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 1141 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 1201 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 1261 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 1321 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 1381 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 1441 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 1501 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 1561 ggcatggacg agctgtacaa gtaa //