LOCUS       BBa_K1067008            1947 bp    DNA     linear       22-Nov-2024
DEFINITION  mCherry downstream tight pBAD
ACCESSION   BBa_K1067008
VERSION     BBa_K1067008.1  
FEATURES             Location/Qualifiers
     CDS             1..880
                     /label=araC
     misc_feature    908..924
                     /label=araO2
     misc_feature    1045..1061
                     /label=araO1L
     misc_feature    1066..1082
                     /label=araO1R
     misc_feature    1087..1108
                     /label=CRP binding site
     misc_feature    1119..1135
                     /label=araI1
     misc_feature    1140..1157
                     /label=araI2
     promoter        1153..1189
                     /label=Tight pBAD promoter
     misc_feature    1153..1158
                     /label=-35
     misc_feature    1177..1182
                     /label=-10
     misc_feature    1..1209
                     /label=BBa_K1067007
     CDS             1237..1947
                     /label=mCherry
     RBS             1225..1229
                     /label=rbs
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgcc ccgaatcagt agtatttatt atctagacat acccgttttt
     1201 tgggctaact ggaattcatt aaagaggaga aaggtaatgg tgagcaaggg cgaggaggat
     1261 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     1321 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1381 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     1441 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     1501 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     1561 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     1621 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     1681 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     1741 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     1801 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     1861 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     1921 ggcggcatgg acgagctgta caagtaa
//