LOCUS       BBa_K1067009            2801 bp    DNA     linear       22-Nov-2024
DEFINITION  Periplasm transport visualization device
ACCESSION   BBa_K1067009
VERSION     BBa_K1067009.1  
FEATURES             Location/Qualifiers
     CDS             1..880
                     /label=araC
     misc_feature    908..924
                     /label=araO2
     misc_feature    1045..1061
                     /label=araO1L
     misc_feature    1066..1082
                     /label=araO1R
     misc_feature    1087..1108
                     /label=CRP binding site
     misc_feature    1119..1135
                     /label=araI1
     misc_feature    1140..1157
                     /label=araI2
     promoter        1153..1189
                     /label=Tight pBAD promoter
     misc_feature    1153..1158
                     /label=-35
     misc_feature    1177..1182
                     /label=-10
     misc_feature    1..1209
                     /label=BBa_K1067007
     misc_feature    1240..1356
                     /label=TAT signal peptide
     misc_feature    1240..1242
                     /label=start
     CDS             1357..2070
                     /label=GFP SF
     misc_feature    2068..2070
                     /label=stop
     misc_feature    2071..2090
                     /label=Gap
     RBS             2079..2083
                     /label=rbs
     misc_feature    2091..2093
                     /label=start
     CDS             2091..2801
                     /label=RFP mCherry
     misc_feature    2799..2801
                     /label=stop
     misc_feature    1218..2801
                     /label=BBa_K1067001
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgcc ccgaatcagt agtatttatt atctagacat acccgttttt
     1201 tgggctaact actagagttc attaaagagg agaaaggtaa tgaataataa cgacctgttt
     1261 caggcgtcgc gtcgacgatt cctcgcacag ctaggtgggt tgacagtggc gggaatgtta
     1321 gggccgtcgc tcctaactcc tcgacgggct acagcccgta aaggcgaaga gctgttcact
     1381 ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa gttttccgtg
     1441 cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt catctgtact
     1501 actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta tggtgttcag
     1561 tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc cgccatgccg
     1621 gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta caaaacgcgt
     1681 gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa aggcattgac
     1741 tttaaagaag acggcaatat cctgggccat aagctggaat acaattttaa cagccacaat
     1801 gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa aattcgccac
     1861 aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac tccaatcggt
     1921 gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt tctgtctaaa
     1981 gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc
     2041 acgcatggta tggatgaact gtacaaatga cattaaagag gagaaaggta atggtgagca
     2101 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     2161 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     2221 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     2281 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     2341 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     2401 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     2461 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     2521 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     2581 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     2641 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     2701 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     2761 gccgccactc caccggcggc atggacgagc tgtacaagta a
//