LOCUS BBa_K1072003 3497 bp DNA linear 01-Nov-2024 DEFINITION Lux pL controlled LuxR+Lux pR autoinduceing luxI(lva tag)+Lux pR controlled Ndh ACCESSION BBa_K1072003 VERSION BBa_K1072003.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 misc_feature 1764..1795 /label=LVA CDS 1185..1763 /label=luxI misc_feature 1185..1802 /label=BBa_C0061 misc_feature 1803..1827 /label=Help:Barcodes CDS 1185..1802 /label=BBa_C0061 stem_loop 1847..1890 /label=stem_loop misc_feature 1836..1915 /label=BBa_B0010 terminator 1836..1915 /label=BBa_B0010 stem_loop 1931..1950 /label=T7 TE misc_feature 1957..1957 /label=stop polyA_site 1951..1964 /label=polya misc_feature 1924..1964 /label=BBa_B0012 terminator 1924..1964 /label=BBa_B0012 misc_feature 1973..1992 /label=LuxR/HSL promoter 1992..1997 /label=-35 promoter 2014..2019 /label=-10 misc_feature 2025..2025 /label=start misc_feature 1973..2027 /label=BBa_R0062 promoter 1973..2027 /label=BBa_R0062 misc_feature 2040..2043 /label=conserved RBS 2036..2047 /label=BBa_B0034 CDS 2056..3360 /label=Ndh coding region CDS 2056..3360 /label=BBa_K1072001 stem_loop 3380..3423 /label=stem_loop misc_feature 3369..3448 /label=BBa_B0010 terminator 3369..3448 /label=BBa_B0010 stem_loop 3464..3483 /label=T7 TE misc_feature 3490..3490 /label=stop polyA_site 3484..3497 /label=polya misc_feature 3457..3497 /label=BBa_B0012 terminator 3457..3497 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgact ataatgataa 1201 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 1261 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 1321 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 1381 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 1441 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 1501 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 1561 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 1621 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 1681 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 1741 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 1801 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1921 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag 1981 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagagaaaga 2041 ggagaaatac tagagttgac tacgccattg aaaaagattg tgattgtcgg cggcggtgct 2101 ggtgggctgg aaatggcaac acagctgggg cataagctgg gacgcaagaa aaaagccaaa 2161 attacgctgg tcgatcgtaa ccacagccac ctgtggaaac cgctgctgca cgaagtggcg 2221 actggctcgc ttgatgaagg cgtcgatgcg ttgagctatc tggcccatgc gcgcaatcat 2281 ggtttccagt tccagctggg ttccgtcatt gatattgatc gtgaagcgaa aacaatcact 2341 attgcagaac tgcgcgacga gaaaggtgaa ctgctggttc cggaacgtaa aatcgcctat 2401 gacaccctgg taatggcgct gggtagcacc tctaacgatt tcaatacgcc aggtgtcaaa 2461 gagaactgca ttttcctcga taacccgcac caggcgcgtc gcttccacca ggagatgctg 2521 aatttgttcc tgaaatactc cgccaacctg ggcgcgaatg gcaaagtgaa cattgcgatt 2581 gtcggcggcg gcgcgacggg tgtagaactc tccgctgaat tgcacaacgc ggtcaagcaa 2641 ctgcacagct acggttacaa aggcctgacc aacgaagccc tgaacgtaac gctggtagaa 2701 gcgggagaac gtattttgcc tgcgttaccg ccacgtatct ctgctgcggc ccacaacgag 2761 ctaacgaaac ttggcgttcg cgtgctgacg caaaccatgg tcaccagtgc tgatgaaggc 2821 ggcctgcaca ctaaagatgg cgaatatatt gaggctgatc tgatggtatg ggcagccggg 2881 atcaaagcgc cagacttcct gaaagatatc ggtggtcttg aaactaaccg tatcaaccag 2941 ctggtggtgg aaccgacgct gcaaaccacc cgcgatccag acatttacgc tattggcgac 3001 tgcgcgtcat gcccgcgtcc ggaagggggc tttgttccgc cgcgtgctca ggctgcacac 3061 cagatggcga cttgcgcaat gaacaacatt ctggcgcaga tgaacggtaa gccgctgaaa 3121 aattatcagt ataaagatca tggttcgctg gtatcgctgt cgaacttctc caccgtcggt 3181 agcctgatgg gtaacctgac gcgcggctca atgatgattg aaggacgaat tgcgcgcttt 3241 gtatatatct cgctataccg aatgcatcag attgcgctgc atggttactt taaaaccgga 3301 ttaatgatgc tggtggggag tattaaccgc gttatccgtc cgcgtttgaa gttgcattaa 3361 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 3421 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 3481 gcctttctgc gtttata //