BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
true
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2046
1
-35
range2046
1
20
25
annotation7070
1
BBa_R0062
range7070
1
1
55
annotation2047
1
-10
range2047
1
42
47
annotation2048
1
start
range2048
1
53
53
annotation2045
1
LuxR/HSL
range2045
1
1
20
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1072004
1
BBa_K1072004
alpha-ALS Coding Region
2013-09-06T11:00:00Z
2015-05-08T01:09:01Z
-
BBa_K1072004 is the conding region of α-acetolactate synthetase(α-ALS).
false
false
_1381_
0
16002
9
It's complicated
false
-
false
Zewei yan, Yichong wang and Junjie zhang
annotation2335232
1
alpha-ALS
range2335232
1
1
1665
BBa_K1072006
1
BBa_K1072006
Lux pR+RBS+α-ALS+2TM
2013-09-06T11:00:00Z
2015-05-08T01:09:01Z
-
α-ALS gene placed under the control of Lux pR promoter. After co-transforming with <bbpart>BBa_K1072000 </bbpart> into E.coli cells,the luxR and AHL complex will activate the Lux pR promotor and transcript α-ALS and GFP genes.
false
false
_1381_
0
16002
9
It's complicated
true
-
false
Ruichen, Yichong wang and Junjie zhang
component2352699
1
BBa_B0012
component2352694
1
BBa_B0034
component2352696
1
BBa_K1072004
component2352697
1
BBa_B0010
component2352688
1
BBa_R0062
annotation2352688
1
BBa_R0062
range2352688
1
1
55
annotation2352694
1
BBa_B0034
range2352694
1
64
75
annotation2352697
1
BBa_B0010
range2352697
1
1755
1834
annotation2352699
1
BBa_B0012
range2352699
1
1843
1883
annotation2352696
1
BBa_K1072004
range2352696
1
82
1746
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0062_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1072006_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgtctgagaaacaatttggggcgaacttggttgtcgatagtttgattaaccataaagtgaagtatgtatttgggattccaggagcaaaaattgaccgggtttttgatttattagaaaatgaagaaggccctcaaatggtcgtgactcgtcatgagcaaggagctgctttcatggctcaagctgttggtcgtttaactggcgaacctggtgtagtagttgttacgagtgggcctggtgtatcaaaccttgcgactccgcttttaaccgcgacatcagaaggtgatgctattttggctatcggtggacaagttaaacgaagtgaccgtcttaaacgtgcacatcaatcaatggataatgctggaatgatgcaatcagcaacaaaatattcagcagaagttcttgaccctgatacactttctgaatcaattgccaacgcttatcgtattgcaaaatcaggacatccaggtgcaactttcttatcaatcccccaagatataacggatgccgaagtatcaatcaaggccattcaaccactttcagaccctaaaatggggaatgcctctattgatgacattaactatttagcacaagcaattaaaaatgctgtattgccagtaattttggttggagctggtgcttcagatgctaaagtcgcttcatcattgcgtaatctattgactcatgttaatattcctgtcgttgaaacattccaaggtgcaggggttatttcacatgatttagaacatactttttatggacgtatcggacttttccgcaatcaaccaggagatatgcttttgaagcgttctgaccttgttattgctgttggttacgacccaattgaatatgaagctcgtaactggaatgcagaaattgatagtcgaattatcgttattgataatgccattgctgaaattgatacttactaccaaccagagcgtgaattaattggtgatatcgcagcaacattggataatcttttaccggctgttcgtggctacaaaattccaaaaggaacaaaagattatctcgatgggcttcatgaagttgctgagcaacacgaatttgatactgaaaatactgaagaaggtagaatgcaccctcttgatttggtcagcactttccaagaaatcgtcaaagatgatgaaacagtaaccgttgacgtaggttcactctacatttggatggcacgtcatttcaaatcatacgaaccacgtcatctcctcttctcaaacggaatgcaaacacttggagttgcacttccttgggcaattacagccgcattgttgcgcccaggtaaaaaagtttattcacactctggtgatggaggtttccttttcacagggcaagaattggaaacagctgtacgtttgaatcttccaatcgttcaaattatctggaatgacggccattatgatatggttaaattccaagaagaaatgaaatatggtcgttcagcagccgttgattttggctatgttgattacgtaaaatatgctgaagcaatgggagcaaaaggttaccgtgcacacagcaaagaagaacttgctgaaattcttaaatcaatcccagatactactggaccagtagtaattgacgttcctttggactattctgataacattaaattagcagaaaaattattgccagaagaattttactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1072004_sequence
1
atgtctgagaaacaatttggggcgaacttggttgtcgatagtttgattaaccataaagtgaagtatgtatttgggattccaggagcaaaaattgaccgggtttttgatttattagaaaatgaagaaggccctcaaatggtcgtgactcgtcatgagcaaggagctgctttcatggctcaagctgttggtcgtttaactggcgaacctggtgtagtagttgttacgagtgggcctggtgtatcaaaccttgcgactccgcttttaaccgcgacatcagaaggtgatgctattttggctatcggtggacaagttaaacgaagtgaccgtcttaaacgtgcacatcaatcaatggataatgctggaatgatgcaatcagcaacaaaatattcagcagaagttcttgaccctgatacactttctgaatcaattgccaacgcttatcgtattgcaaaatcaggacatccaggtgcaactttcttatcaatcccccaagatataacggatgccgaagtatcaatcaaggccattcaaccactttcagaccctaaaatggggaatgcctctattgatgacattaactatttagcacaagcaattaaaaatgctgtattgccagtaattttggttggagctggtgcttcagatgctaaagtcgcttcatcattgcgtaatctattgactcatgttaatattcctgtcgttgaaacattccaaggtgcaggggttatttcacatgatttagaacatactttttatggacgtatcggacttttccgcaatcaaccaggagatatgcttttgaagcgttctgaccttgttattgctgttggttacgacccaattgaatatgaagctcgtaactggaatgcagaaattgatagtcgaattatcgttattgataatgccattgctgaaattgatacttactaccaaccagagcgtgaattaattggtgatatcgcagcaacattggataatcttttaccggctgttcgtggctacaaaattccaaaaggaacaaaagattatctcgatgggcttcatgaagttgctgagcaacacgaatttgatactgaaaatactgaagaaggtagaatgcaccctcttgatttggtcagcactttccaagaaatcgtcaaagatgatgaaacagtaaccgttgacgtaggttcactctacatttggatggcacgtcatttcaaatcatacgaaccacgtcatctcctcttctcaaacggaatgcaaacacttggagttgcacttccttgggcaattacagccgcattgttgcgcccaggtaaaaaagtttattcacactctggtgatggaggtttccttttcacagggcaagaattggaaacagctgtacgtttgaatcttccaatcgttcaaattatctggaatgacggccattatgatatggttaaattccaagaagaaatgaaatatggtcgttcagcagccgttgattttggctatgttgattacgtaaaatatgctgaagcaatgggagcaaaaggttaccgtgcacacagcaaagaagaacttgctgaaattcttaaatcaatcccagatactactggaccagtagtaattgacgttcctttggactattctgataacattaaattagcagaaaaattattgccagaagaattttactga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z