LOCUS       BBa_K1072012            3488 bp    DNA     linear       17-May-2024
DEFINITION  Pgpa1+Chimeric Gpa1(Gα subunit)+Rfp+ADH1
ACCESSION   BBa_K1072012
VERSION     BBa_K1072012.1  
FEATURES             Location/Qualifiers
     promoter        1..1000
                     /label=pGpa1
     CDS             1001..2419
                     /label=Gpa1 Coding region
     misc_feature    1..2419
                     /label=BBa_K1072011
     CDS             2426..3100
                     /label=mrfp1
     misc_feature    3107..3131
                     /label=Help:Barcodes
     CDS             2426..3131
                     /label=BBa_E1010
     misc_feature    3140..3488
                     /label=ADH1-Terminator
     terminator      3140..3488
                     /label=BBa_K801012
ORIGIN
        1 tcatacttag aaaaacatgt ctttcttagg tttcggtggt ggtcagcctc aattatcatc
       61 tcaacaaaag attcaagctg cggaagctga actagatttg gtcacagaca tgttcaataa
      121 attggttaat aactgttata aaaaatgtat caatacttct tattccgagg gtgagctgaa
      181 taagaatgaa tcttcgtgcc tagacagatg tgtggccaaa tattttgaga ccaatgttca
      241 agtcggtgaa aacatgcaga aaatgggcca atcatttaac gcagccggta agttttagaa
      301 atgtgcatta aagcagtaat gataagacga aaataagaaa agaaatccat aagctgtttt
      361 actcgactca acgttataat tagtattatt gattcatatc ctgtatatac aagtaacatt
      421 atactctttt ctgtacttca tctttacctt tttatcttac atggcacatg ttgtttgaaa
      481 acaagatcat aggtggataa agcaagccga atctaaaaaa aaaaaaatgt ctctattgga
      541 aaactgaatg cataacgata ttcccttttc atgcagataa cactggcttg tttcaatttg
      601 aaacgcatct tcgtgttatt tcaccgaaac gcactcggct cagcatgtta aaaagcacat
      661 caatttaggg ctctgcgcgt ccttctgcgt attcttcctt gtagaaatgc aattaaatgg
      721 agagcagaaa tttttttgtt acatattgtt ttccttaaag ggaaatatta aaaaatagtc
      781 taaaatgaag aggatagtag tagtccacca atttctttac gttttatatt attcgtaatc
      841 ttttgatctg ttattcattt tttcttgtca ctccgtttct aacatttttg accatttcta
      901 agaccaaact gagtagaagc tattcatact gtaaattggt attttagcat cacatcaata
      961 atccagaggt gtataaattg atatattaag gtaggaaata atggggtgta cagtgagtac
     1021 gcaaacaata ggagacgaaa gtgatccttt tctacagaac aaaagagcca atgatgtcat
     1081 cgagcaatcg ttgcagctgg agaaacaacg tgacaagaat gaaataaaac tgttactatt
     1141 aggtgccggt gagtcaggta aatcaacggt tttaaaacaa ttaaaattat tacatcaagg
     1201 cggtttctcc catcaagaaa ggttacagta tgctcaagtg atatgggcag atgccataca
     1261 atcaatgaaa attttgatta ttcaggccag aaaactaggt attcaacttg actgtgatga
     1321 tccgatcaac aataaagatt tgtttgcatg caagagaata ctgctaaagg ctaaagcttt
     1381 agattatatc aacgccagtg ttgccggtgg ttctgatttt ctaaatgatt atgtactgaa
     1441 gtactcagaa aggtatgaaa ctaggaggcg tgttcagagt accggacgag caaaagctgc
     1501 tttcgatgaa gacggaaata tttctaatgt caaaagtgac actgacagag atgctgaaac
     1561 ggtgacgcaa aatgaggatg ctgatagaaa caacagtagt agaattaacc tacaggatat
     1621 ttgcaaggac ttgaaccaag aaggcgatga ccagatgttt gttagaaaaa catcaaggga
     1681 aattcaagga caaaatagac gaaatcttat tcacgaagac attgctaagg caataaagca
     1741 actttggaat aacgacaaag gtataaagca gtgttttgca cgttctaatg agtttcaatt
     1801 ggagggctca gctgcatact actttgataa cattgagaaa tttgctagtc cgaattatgt
     1861 ctgtacggat gaagacattt tgaagggccg tataaagact acaggcatta cagaaaccga
     1921 atttaacatc ggctcgtcca aattcaaggt tctcgacgct ggtgggcagc gttctgaacg
     1981 taagaagtgg attcattgtt tcgaaggaat tacagcagtt ttatttgttt tagcaatgag
     2041 tgaatacgac cagatgttgt ttgaggatga aagagtgaac agaatgcatg aatcaataat
     2101 gctatttgac acgttattga actctaagtg gttcaaagat acaccgttta ttttgttttt
     2161 aaataaaatt gatttgttcg aggaaaaggt aaaaagcatg cccataagaa agtactttcc
     2221 tgattaccag ggacgtgtcg gcgatgcaga agcgggtcta aaatattttg agaagatatt
     2281 tttgagcttg aataagacaa acaaaccaat ctacgtgaaa cgaacctgcg ctaccgatac
     2341 ccaaactatg aagttcgtat tgagtgcagt caccgatcta atcatccagc aaaaccttaa
     2401 aaaggctgga atgatgtaat actagatggc ttcctccgaa gacgttatca aagagttcat
     2461 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
     2521 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
     2581 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
     2641 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
     2701 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
     2761 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
     2821 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
     2881 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
     2941 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
     3001 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
     3061 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
     3121 gtgtagatcg ctactagaga gctttggact tcttcgccag aggtttggtc aagtctccaa
     3181 tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa
     3241 tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt
     3301 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     3361 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     3421 aggtatagca tgaggtcgct cttattgacc acacctctac cggccggtcg aaattcccct
     3481 accctatg
//