BBa_K801012
1
BBa_K801012
ADH1 yeast terminator
2012-09-20T11:00:00Z
2015-05-08T01:13:24Z
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
Released HQ 2013
ADH1 terminator of ''S. cerevisiae''
false
false
_1057_
0
13624
9
In stock
false
-
false
Georg Sch??tzinger
annotation2202145
1
ADH1-Terminator
range2202145
1
1
349
BBa_K1072011
1
BBa_K1072011
Pgpa1+Chimeric Gpa1(Gα subunit)
2013-09-07T11:00:00Z
2015-05-08T01:09:01Z
Originate from C. elegans
In yeast, the pheromone receptors are coupled to a heterotrimeric G protein. This is composed of Gpa1p (Gα), Ste4p (Gβ) and Ste18p(Gγ) subunits. In order to coupling heterologous GPCRs in yeast successfully, ???Transplants??? have the five C-terminal residues of Odr3 of C. elegans, replaced with the corresponding mammalian Gα residues(Brown et al. 2000).
false
false
_1381_
0
16002
9
Not in stock
false
-
false
Junqi Kuang, Yaqing du, Xihao liao and Junjie zhang
annotation2335452
1
pGpa1
range2335452
1
1
1000
annotation2335453
1
Gpa1 Coding region
range2335453
1
1001
2419
BBa_E1010
1
mRFP1
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
2004-07-27T11:00:00Z
2015-08-31T04:07:26Z
Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a>
Released HQ 2013
monomeric RFP:
Red Fluorescent Protein.
Excitation peak: 584 nm
Emission peak: 607 nm
false
false
_11_1_
0
52
7
In stock
false
TAATAA double stop codon added (DE).
Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
true
Drew Endy
annotation1014044
1
mrfp1
range1014044
1
1
675
annotation2214014
1
Help:Barcodes
range2214014
1
682
706
BBa_K1072012
1
BBa_K1072012
Pgpa1+Chimeric Gpa1(Gα subunit)+Rfp+ADH1
2013-09-07T11:00:00Z
2015-05-08T01:09:01Z
-
In yeast, as in mammalian cells, the pheromone receptors are coupled to a heterotrimeric G protein. This is composed of Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits. In order to coupling heterologous GPCRs in yeast, ???Transplants??? have the five C-terminal residues,originating from a C. elegans, G- protein, Odr3, replaced with the corresponding mammalian Gα residues.
<html>
<img src="http://parts.igem.org/wiki/images/archive/2/20/20130908135351%21SCUT13_Olfactory_receptor_BFP.jpg" style="width:700px;margin-left:10%;"/>
</html>
<p>Figure 1. Comperison between endogenous GPCR pathway in yeast and motified Odr10 pathway.</p>
<br />
a repetor gene, Rfp, and ADH1 terminator have been added to de <bbpart>BBa_K1072011</bbpart>
false
false
_1381_
0
16002
9
In stock
true
-
false
Junqi kuang, Yaqing du and Junjie zhang
component2350583
1
BBa_E1010
component2350580
1
BBa_K1072011
component2350585
1
BBa_K801012
annotation2350583
1
BBa_E1010
range2350583
1
2426
3131
annotation2350580
1
BBa_K1072011
range2350580
1
1
2419
annotation2350585
1
BBa_K801012
range2350585
1
3140
3488
BBa_K801012_sequence
1
agctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg
BBa_E1010_sequence
1
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
BBa_K1072012_sequence
1
tcatacttagaaaaacatgtctttcttaggtttcggtggtggtcagcctcaattatcatctcaacaaaagattcaagctgcggaagctgaactagatttggtcacagacatgttcaataaattggttaataactgttataaaaaatgtatcaatacttcttattccgagggtgagctgaataagaatgaatcttcgtgcctagacagatgtgtggccaaatattttgagaccaatgttcaagtcggtgaaaacatgcagaaaatgggccaatcatttaacgcagccggtaagttttagaaatgtgcattaaagcagtaatgataagacgaaaataagaaaagaaatccataagctgttttactcgactcaacgttataattagtattattgattcatatcctgtatatacaagtaacattatactcttttctgtacttcatctttacctttttatcttacatggcacatgttgtttgaaaacaagatcataggtggataaagcaagccgaatctaaaaaaaaaaaaatgtctctattggaaaactgaatgcataacgatattcccttttcatgcagataacactggcttgtttcaatttgaaacgcatcttcgtgttatttcaccgaaacgcactcggctcagcatgttaaaaagcacatcaatttagggctctgcgcgtccttctgcgtattcttccttgtagaaatgcaattaaatggagagcagaaatttttttgttacatattgttttccttaaagggaaatattaaaaaatagtctaaaatgaagaggatagtagtagtccaccaatttctttacgttttatattattcgtaatcttttgatctgttattcattttttcttgtcactccgtttctaacatttttgaccatttctaagaccaaactgagtagaagctattcatactgtaaattggtattttagcatcacatcaataatccagaggtgtataaattgatatattaaggtaggaaataatggggtgtacagtgagtacgcaaacaataggagacgaaagtgatccttttctacagaacaaaagagccaatgatgtcatcgagcaatcgttgcagctggagaaacaacgtgacaagaatgaaataaaactgttactattaggtgccggtgagtcaggtaaatcaacggttttaaaacaattaaaattattacatcaaggcggtttctcccatcaagaaaggttacagtatgctcaagtgatatgggcagatgccatacaatcaatgaaaattttgattattcaggccagaaaactaggtattcaacttgactgtgatgatccgatcaacaataaagatttgtttgcatgcaagagaatactgctaaaggctaaagctttagattatatcaacgccagtgttgccggtggttctgattttctaaatgattatgtactgaagtactcagaaaggtatgaaactaggaggcgtgttcagagtaccggacgagcaaaagctgctttcgatgaagacggaaatatttctaatgtcaaaagtgacactgacagagatgctgaaacggtgacgcaaaatgaggatgctgatagaaacaacagtagtagaattaacctacaggatatttgcaaggacttgaaccaagaaggcgatgaccagatgtttgttagaaaaacatcaagggaaattcaaggacaaaatagacgaaatcttattcacgaagacattgctaaggcaataaagcaactttggaataacgacaaaggtataaagcagtgttttgcacgttctaatgagtttcaattggagggctcagctgcatactactttgataacattgagaaatttgctagtccgaattatgtctgtacggatgaagacattttgaagggccgtataaagactacaggcattacagaaaccgaatttaacatcggctcgtccaaattcaaggttctcgacgctggtgggcagcgttctgaacgtaagaagtggattcattgtttcgaaggaattacagcagttttatttgttttagcaatgagtgaatacgaccagatgttgtttgaggatgaaagagtgaacagaatgcatgaatcaataatgctatttgacacgttattgaactctaagtggttcaaagatacaccgtttattttgtttttaaataaaattgatttgttcgaggaaaaggtaaaaagcatgcccataagaaagtactttcctgattaccagggacgtgtcggcgatgcagaagcgggtctaaaatattttgagaagatatttttgagcttgaataagacaaacaaaccaatctacgtgaaacgaacctgcgctaccgatacccaaactatgaagttcgtattgagtgcagtcaccgatctaatcatccagcaaaaccttaaaaaggctggaatgatgtaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagagctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg
BBa_K1072011_sequence
1
tcatacttagaaaaacatgtctttcttaggtttcggtggtggtcagcctcaattatcatctcaacaaaagattcaagctgcggaagctgaactagatttggtcacagacatgttcaataaattggttaataactgttataaaaaatgtatcaatacttcttattccgagggtgagctgaataagaatgaatcttcgtgcctagacagatgtgtggccaaatattttgagaccaatgttcaagtcggtgaaaacatgcagaaaatgggccaatcatttaacgcagccggtaagttttagaaatgtgcattaaagcagtaatgataagacgaaaataagaaaagaaatccataagctgttttactcgactcaacgttataattagtattattgattcatatcctgtatatacaagtaacattatactcttttctgtacttcatctttacctttttatcttacatggcacatgttgtttgaaaacaagatcataggtggataaagcaagccgaatctaaaaaaaaaaaaatgtctctattggaaaactgaatgcataacgatattcccttttcatgcagataacactggcttgtttcaatttgaaacgcatcttcgtgttatttcaccgaaacgcactcggctcagcatgttaaaaagcacatcaatttagggctctgcgcgtccttctgcgtattcttccttgtagaaatgcaattaaatggagagcagaaatttttttgttacatattgttttccttaaagggaaatattaaaaaatagtctaaaatgaagaggatagtagtagtccaccaatttctttacgttttatattattcgtaatcttttgatctgttattcattttttcttgtcactccgtttctaacatttttgaccatttctaagaccaaactgagtagaagctattcatactgtaaattggtattttagcatcacatcaataatccagaggtgtataaattgatatattaaggtaggaaataatggggtgtacagtgagtacgcaaacaataggagacgaaagtgatccttttctacagaacaaaagagccaatgatgtcatcgagcaatcgttgcagctggagaaacaacgtgacaagaatgaaataaaactgttactattaggtgccggtgagtcaggtaaatcaacggttttaaaacaattaaaattattacatcaaggcggtttctcccatcaagaaaggttacagtatgctcaagtgatatgggcagatgccatacaatcaatgaaaattttgattattcaggccagaaaactaggtattcaacttgactgtgatgatccgatcaacaataaagatttgtttgcatgcaagagaatactgctaaaggctaaagctttagattatatcaacgccagtgttgccggtggttctgattttctaaatgattatgtactgaagtactcagaaaggtatgaaactaggaggcgtgttcagagtaccggacgagcaaaagctgctttcgatgaagacggaaatatttctaatgtcaaaagtgacactgacagagatgctgaaacggtgacgcaaaatgaggatgctgatagaaacaacagtagtagaattaacctacaggatatttgcaaggacttgaaccaagaaggcgatgaccagatgtttgttagaaaaacatcaagggaaattcaaggacaaaatagacgaaatcttattcacgaagacattgctaaggcaataaagcaactttggaataacgacaaaggtataaagcagtgttttgcacgttctaatgagtttcaattggagggctcagctgcatactactttgataacattgagaaatttgctagtccgaattatgtctgtacggatgaagacattttgaagggccgtataaagactacaggcattacagaaaccgaatttaacatcggctcgtccaaattcaaggttctcgacgctggtgggcagcgttctgaacgtaagaagtggattcattgtttcgaaggaattacagcagttttatttgttttagcaatgagtgaatacgaccagatgttgtttgaggatgaaagagtgaacagaatgcatgaatcaataatgctatttgacacgttattgaactctaagtggttcaaagatacaccgtttattttgtttttaaataaaattgatttgttcgaggaaaaggtaaaaagcatgcccataagaaagtactttcctgattaccagggacgtgtcggcgatgcagaagcgggtctaaaatattttgagaagatatttttgagcttgaataagacaaacaaaccaatctacgtgaaacgaacctgcgctaccgatacccaaactatgaagttcgtattgagtgcagtcaccgatctaatcatccagcaaaaccttaaaaaggctggaatgatgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z