BBa_K801012 1 BBa_K801012 ADH1 yeast terminator 2012-09-20T11:00:00Z 2015-05-08T01:13:24Z Extracted from genomic DNA of ''S. cerevisiae'' using PCR. Released HQ 2013 ADH1 terminator of ''S. cerevisiae'' false false _1057_ 0 13624 9 In stock false - false Georg Sch??tzinger annotation2202145 1 ADH1-Terminator range2202145 1 1 349 BBa_K1072008 1 BBa_K1072008 Flag tag 2013-09-06T11:00:00Z 2015-05-08T01:09:01Z synthesis This part just is Flag tag. false false _1381_ 0 16002 9 Not in stock false - false Junqi kuang, Xihao liao and Jianhui gong annotation2335360 1 Flag tag range2335360 1 1 27 BBa_K1072013 1 BBa_K1072013 pGAL1+ Flag + odr-10 + GFP + ADH1 Te 2013-09-08T11:00:00Z 2015-05-08T01:09:01Z Caenorhabditis elegans olfactory receptor Odr-10 BBa_K1072013 is protein generator, including Odr-10 and GFP; BBa_K1072013 encodes a diacetyl-sensing G-protein coupled receptor, 7-transmembrane receptors, which can be functionally expressed in S. cerevisiae. We add Flag tag in C-terminal and link GFP coding region in N-terminal. This part is an improvement of <bbpart>BBa_K211002</bbpart> and <bbpart>BBa_K775003<bbpart> made by 2009 Hongkong university and 2012 TU delft team, respectively. A Flag tag and GFP have been added. false false _1381_ 0 16002 9 In stock true As discription of most paper, GPCR,odr-10, false Junqi kuang, Yaqing du and Xihao liao component2406351 1 BBa_K1072010 component2406349 1 BBa_K1072008 component2406347 1 BBa_J63006 component2406355 1 BBa_K801012 component2406353 1 BBa_E0040 annotation2406351 1 BBa_K1072010 range2406351 1 589 1608 annotation2406349 1 BBa_K1072008 range2406349 1 556 582 annotation2406353 1 BBa_E0040 range2406353 1 1615 2334 annotation2406347 1 BBa_J63006 range2406347 1 1 549 annotation2406355 1 BBa_K801012 range2406355 1 2343 2691 BBa_K1072010 1 BBa_K1072010 Odr-10(diacetyl)GPCR with optimized codon 2013-09-08T11:00:00Z 2015-05-08T01:09:01Z Originate from the C. elegans. Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans. If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein, Odr-10. pathway is composedof Gpa1p (G&#945;), Ste4p (G&#946;) and Ste18p (G&#947;) subunits.The G&#946;&#947; particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will link a reproter, such as GFP, His3 or LacZ. false false _1381_ 0 16002 9 Not in stock false As discription of most paper, RNA of Odr-10 be used as plate and transform into receptor cells to express member protein successfully. false Junqi kuang, Xihao liao and Jianhui gong annotation2357697 1 Odr-10 coding region range2357697 1 1 1020 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_J63006 1 GAL1 yeast GAL1 promoter 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic GAL1 locus Released HQ 2013 GAL1 promoter from S. cerevisiae false true _97_ 0 545 97 In stock false unchanged from genomic sequence true Caroline Ajo-Franklin BBa_K801012_sequence 1 agctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg BBa_J63006_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1072008_sequence 1 atggattacaaagatgatgatgataag BBa_K1072010_sequence 1 atgtctggtgaattgtggataaccttagttgatactgctgacatcgtcggtgtaaccttgactttttgtgtaaacatagttttgttgggtttgttgaagactagaggtaaaaatttgggtacatacaagtacttaatggcatttttctccgtttttagtattttctatgccatcatcgagtttattttaagacctataatgcatatcgaaaacactacatttttcttgatctccagaaagagattcaattacagtaccaagttgggtaaaattaattcagctttctactgtgcatgcttcgccacttcttttgttgtctcaggtgtccacttcgtatatagatactttgcaacctgtaagcctaatttgttgagattgttcaacttaccaactttgttgttgtggcctttgggttgctccgttccagtcacaatgtgggcctctgtttcatactttttgtacccagatacagaatataccgaagctgctgttacaaacgtcttgaacaaccattacaactggatcaaaaaggaaaacgtcagttacatcgcatacgtatactaccaatacgaaaacggtgttagacatatatatttgaaaaatttgttgggttgtttcgtacactacttcgttatgtctatgacattcgtagttatgttctattgcggttacgctacttggaaaacaatgaacgaacacaaggatgtttcagacagaaccagagctttgcaaaagcaattgtttaaagcattagttttgcaaacattgatccctaccattttcatgtatgccccaacaggtgttatgtttattgctccatttttcgatgtcaatttgaacgccaacgctaacttcatcgttttctgttcctttttgtacccaggtttagatcctttgatcttgatcttgatcataagagacttcagaagaactatttttaatttcttgtgcggtaaaaagaacagtgtcgatgaatccagatccacaaccagagccaacttatcccaagtaccaacctaa BBa_K1072013_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatggattacaaagatgatgatgataagtactagatgtctggtgaattgtggataaccttagttgatactgctgacatcgtcggtgtaaccttgactttttgtgtaaacatagttttgttgggtttgttgaagactagaggtaaaaatttgggtacatacaagtacttaatggcatttttctccgtttttagtattttctatgccatcatcgagtttattttaagacctataatgcatatcgaaaacactacatttttcttgatctccagaaagagattcaattacagtaccaagttgggtaaaattaattcagctttctactgtgcatgcttcgccacttcttttgttgtctcaggtgtccacttcgtatatagatactttgcaacctgtaagcctaatttgttgagattgttcaacttaccaactttgttgttgtggcctttgggttgctccgttccagtcacaatgtgggcctctgtttcatactttttgtacccagatacagaatataccgaagctgctgttacaaacgtcttgaacaaccattacaactggatcaaaaaggaaaacgtcagttacatcgcatacgtatactaccaatacgaaaacggtgttagacatatatatttgaaaaatttgttgggttgtttcgtacactacttcgttatgtctatgacattcgtagttatgttctattgcggttacgctacttggaaaacaatgaacgaacacaaggatgtttcagacagaaccagagctttgcaaaagcaattgtttaaagcattagttttgcaaacattgatccctaccattttcatgtatgccccaacaggtgttatgtttattgctccatttttcgatgtcaatttgaacgccaacgctaacttcatcgttttctgttcctttttgtacccaggtttagatcctttgatcttgatcttgatcataagagacttcagaagaactatttttaatttcttgtgcggtaaaaagaacagtgtcgatgaatccagatccacaaccagagccaacttatcccaagtaccaacctaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagagctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z